Preliminary evaluation of the impact of preharvest UV-C on lettuce: potential for the control of <i>Xanthomonas campestris</i>

Nicolas, Olbert; Charles, Marie Thérèse; Chabot, D.; Aarrouf, Jawad; Jenni, Sylvie; Toussaint, Vicky; Beaulieu, Carole

doi:10.17660/actahortic.2020.1271.53

Cited by 3 publications

(2 citation statements)

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“…This work confirms that a UV-C treatment of 1.6 kJ/m 2 applied as described by Nicolas et al (2020) falls within the hormetic dose range conferring a protective effect on lettuce against BLS. This is consistent with previous studies that have reported the efficacy of hermetic treatment of UV-C radiation in reducing the symptoms observed in a variety of plant-pathogen interactions (Janisiewicz et al, 2016a,b;Xu et al, 2019;Aarrouf and Urban, 2020;Nicolas et al, 2020;Vàsquez et al, 2020). Figure 6, which served as a framework for the discussion below, was developed using original data from the present study and available data from the literature.…”

Section: Discussionsupporting

confidence: 81%

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Preharvest UV-C Hormesis Induces Key Genes Associated With Homeostasis, Growth and Defense in Lettuce Inoculated With Xanthomonas campestris pv. vitians

et al. 2022

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Preharvest application of hormetic doses of ultraviolet-C (UV-C) generates beneficial effects in plants. In this study, within 1 week, four UV-C treatments of 0.4 kJ/m2 were applied to 3-week-old lettuce seedlings. The leaves were inoculated with a virulent strain of Xanthomonas campestris pv. vitians (Xcv) 48 h after the last UV-C application. The extent of the disease was tracked over time and a transcriptomic analysis was performed on lettuce leaf samples. Samples of lettuce leaves, from both control and treated groups, were taken at two different times corresponding to T2, 48 h after the last UV-C treatment and T3, 24 h after inoculation (i.e., 72 h after the last UV-C treatment). A significant decrease in disease severity between the UV-C treated lettuce and the control was observed on days 4, 8, and 14 after pathogen inoculation. Data from the transcriptomic study revealed, that in response to the effect of UV-C alone and/or UV-C + Xcv, a total of 3828 genes were differentially regulated with fold change (|log2-FC|) > 1.5 and false discovery rate (FDR) < 0.05. Among these, of the 2270 genes of known function 1556 were upregulated and 714 were downregulated. A total of 10 candidate genes were verified by qPCR and were generally consistent with the transcriptomic results. The differentially expressed genes observed in lettuce under the conditions of the present study were associated with 14 different biological processes in the plant. These genes are involved in a series of metabolic pathways associated with the ability of lettuce treated with hormetic doses of UV-C to resume normal growth and to defend themselves against potential stressors. The results indicate that the hormetic dose of UV-C applied preharvest on lettuce in this study, can be considered as an eustress that does not interfere with the ability of the treated plants to carry on a set of key physiological processes namely: homeostasis, growth and defense.

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Section: Discussionsupporting

confidence: 81%

“…The time required to administer the treatment dose of 0.4 kJ/m 2 was 1 min. Four UV-C doses of 0.4 kJ/m 2 ( Supplementary Figure 1 ) at intervals of 48 h between the first three doses and 72 h for the last dose was applied ( Nicolas et al, 2020 ). In total, lettuce plants in the treated group received 1.6 kJ/m 2 over a 1-week period.…”

Section: Methodsmentioning

confidence: 99%