Preliminary evaluation of resistance genes in rice against bacterial leaf blight in Guilan Province—Iran

Abstract

The reactions of rice bacterial leaf blight races were identified in Guilan province-Iran on 12 near-isogenic lines and 14 pyramiding lines from International Network for Genetic Evaluation of rice (INGER) and 8 local and improved Iranian varieties were evaluated under natural photoperiod condition in the field. Inoculation was done at panicle initiation by clipping the sterilized scissors in the bacterial suspension to booting stage. Scoring of inoculated plants was made 21 days after inoculation. Infection levels of pyramiding lines containing two to five resistance genes, expect, IRBB53 and IRBB61 with respectively resistance gene combination, Xa5 + Xa13 and Xa4 + Xa5 + Xa7, were not so clear. Among near-isogenic lines IRBB1, IRBB2, IRBB4 and IRBB10 carrying resistance gene Xa1, Xa2, Xa4 and Xa10 were susceptible; IRBB8, IRBB11, IRBB3, IRBB5 and IRBB13 were moderately susceptible; (having resistance gene Xa8, Xa11, Xa3, Xa5 and Xa13) IRBB14, IRBB21 and IRBB7 with respectively resistance gene Xa14, Xa21 and Xa7 were moderately resistance to bacterial blight. Furthermore, most of the time gene combinations support the strategy of pyramiding appropriate resistance gene. Local varieties were more susceptible than improved varieties to leaf blight disease. Among local varieties, Tarom was the most susceptible. And also, there were no significant differences among improved varieties and all of them were moderately resistance.

APPRAISAL OF RESISTANT GENES AND GENE PYRAMID LINES OF RICE AGAINST INDIGENOUS PATHOTYPES OF Xanthomonas oryzae pv. oryzae IN PUNJAB, PAKISTAN

“…The Xa7 as a single gene is also an effective gene for deployment in BB resistance in Punjab. It has also been speculated to confer durable resistance in Korea (Jeung et al, 2006), in Indonesia (Utami et al, 2013) and in Iran (Khoshkdaman et al, 2014). It has been found, in this investigation, that the performance of xa5,Xa21) was the best in all pyramids.…”

Ketahanan Varietas Unggul Baru dan Galur Isogenik Padi terhadap Penyakit Hawar Daun Bakteri pada Kondisi Lapangan

“…Varietas unggul ini adalah hasil persilangan antara varietas Ciherang dengan galur IRBB-64 (Jamil et al 2016). Galur IRBB-64 memiliki gen-gen tahan pyramiding, yaitu Xa4+xa5+Xa7+Xa21 (Khoshkdaman et al 2014). Hal ini menyebabkan Inpari-32 konsisten sangat tahan di Cilacap dan Kuningan.…”

“…Hasil penelitian ini menunjukkan galur-galur IRBB-5, IRBB-7, IRBB-21, dan IRBB-57 memiliki ketahanan yang sangat baik terhadap HDB. Hasil penelitian Khoshkdaman et al (2014) menunjukkan galur isogenik IRBB-1, IRBB-3, dan IRBB-4 rentan terhadap penyakit HDB. Namun galur IRBB-7 dan IRBB-21 agak tahan.…”

A novel intragenic marker targeting the ectodomain of bacterial blight-resistance gene Xa21 for marker-assisted selection in rice

“…A 3910-bp genomic sequence of the Xa21 gene of the japonica genomic reference Nipponbare (Os11g0569733; synonym LOC_Os11g36180) and the sequence of the Xa21 ortholog of Oryza longistaminata (KN543217.1_FG001) was retrieved from Gramene Database [50] (http:// www.gramene.org/; Gramene Search UI Build #705.1 accessed on 12/27/2017). Using the locus position of Os11g0569733 (Chr11: 21273533..21277443), the genomic sequence of Xa21 was retrieved for IRBB 62 (IRGC 135947; known-to-carry the resistance allele at Xa21; [51,52] and IRBB 7 (IRGC 135948; known-to-carry the susceptible allele at Xa21; [52,53,54] from the Rice SNP-Seek Database [55] (http://oryzasnp.org/iric-portal/). The retrieved genomic sequences were aligned with multiple sequence alignment function in S2), and was aligned according to the methods described above (hereinafter referred to as Xa21 in silico sequence alignment).…”