This research aimed to determine the composition and pathotype distribution of Xanthomonas oryzae pv. oryzae (Xoo) in several rice producing centers in Indonesia during the growing season of 2010-2013. The studies were conducted into three phases of activities, namely: sampling and collection of infected leaves from the representative rice growing areas; isolation and purification of the Xoo isolates; and evaluation of composition and pathotype of Xoo on five rice differential varieties. Results indicated that a total of 2,658 isolates Xoo have been isolated from 10 provinces representing rice ecosystem in Indonesia during the growing season from 2010-2013. Evaluating these Xoo isolates against five differential varieties revealed that these Xoo isolates consisted of three pathotypes III, IV, and VIII with a total of 30, 36, and 34%, respectively. The data also indicated that two pathotypes III and IV were dominant in three provinces, while the pathotype VIII was dominant in four provinces. As mentioned previously, such information are useful in designing strategy of integrating components of technologies combined in the management of Bacterial leaf blight (BLB) occurrences in a particular endemic areas.
<p>A study was carried out to identify the composition and distribution of Xanthomonas oryzae pv. oryzae (Xoo) pathotypes on rice crop in West Nusa Tenggara, during the 2012 planting season. Three activities were conducted, namely collection of rice leaf samples from the fields, isolation of Xoo from the leaf samples at the laboratory, and testing pathotypes of Xoo at the screen house. Rice leaves showing typical bacterial leaf blight (BLB) symptom were collected from various farmers’ fields. The samples were detached and put into paper envelopes, and were taken to the laboratory for isolation of Xoo, at the Laboratory of Pythopathology of Indonesian Center for Rice Research (ICRR), Sukamandi. Pathotype testing was done in the ICRR screen house by inoculating the leaves of five differential rice varieties using inocula of the Xoo isolates. Resistance of the rice differential varieties was determined based on the BLB disease severity. Inoculated plant with disease severity ≤11% was considered resistant (R) and disease severity >11% was susceptible (S). From the 240 samples of rice leaf infected with BLB collected from West Nusa Tenggara, 232 Xoo isolates were obtained. The Xoo pathotype identification showed that pathotype IV was the most dominant in West Nusa Tenggara during the 2012 planting season, numbering 118 isolates or 51.0% out of the total isolates, followed by pathotype VIII (67 isolates or 29.0%), and pathotype III (47 isolates or 20.0%).</p>
At present, bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae is highly damaging to rice production in Indonesia as most of the existing commercial rice varieties are susceptible to the existing pathotypes of the bacteria. To solve such problems, varietal rotation should be based on information on the existence and dominance of local pathotypes in a particular rice ecosystem. To obtain this information, a total of 117 isolates of X. oryzae pv. oryzae, collected from West Java, Central Java, and Yogyakarta, were evaluated for their pathotype variation on five differential rice varieties during the dry season of 2000. When disease severity was < 10%, the reaction was classified as resistant (R) and when > 11%, was susceptible (S). The data indicated that 3.42% of the isolates were pathotype III, 12.82% were pathotype IV, and 83.76% were pathotype VIII. In West Java, the bacterial pathotypes III, IV, and VIII were 4.94%, 14.81%, and 80.25%, respectively. In low elevation areas, 4.94%, 9.88%, and 45.68% were pathotypes III, IV, and VIII, respectively and in medium areas, 4.94% were pathotypes IV and 34.57% were pathotype VIII. In Central Java, no pathotype III was found, while pathotypes IV and VIII were as much as 4.52% and 90.48%, respectively. In low elevation areas, no pathotypes III was recovered, and a total of 9.53% and 76.19% were identified as pathotypes IV and VIII, respectively, and in medium areas only pathotype VIII (14.29%) was identified. In Yogyakarta, pathotypes IV and VIII were found. In low elevation areas, 6.67% and 93.33% were pathotypes IV and VIII, respectively and in medium areas, 14.29% and 85.71% were pathotypes IV and VIII, respectively. The data indicated that variation in pathotype composition over different locations was obvious and locally specific resistant varieties to the disease are needed in the management of this important bacterial disease in rice.
ABSTRAKBudidaya padi organik bertujuan meminimalkan penggunaan pupuk anorganik dan pestisida kimia, namun kenyataannya tetap tidak terlepas dari gangguan hama dan penyakit. Penelitian bertujuan untuk mengetahui keragaan hama, penyakit, dan musuh alami pada budidaya padi organik. Penelitian dilaksanakan di Screen Field BB Padi pada Musim Hujan (MH) 2015/2016 dan Musim Kemarau (MK) 2016. Rancangan penelitian yang digunakan adalah Split Plot dengan tiga ulangan. Petak utama yaitu varietas: 1). Inpari 25, 2). Inpari 33, dan 3). Sintanur. Anak petak yaitu aplikasi ekstrak daun: 1). Mindi, 2). Sirsak, 3). Mahoni, dan 4). Kontrol. Aplikasi dilakukan pada 4, 6, 8, dan 10 minggu setelah tanam (MST). Pengamatan hama, penyakit, dan musuh alami pada 5, 7, 9, dan 11 MST sebanyak 20 rumpun per plot. Hasil penelitian menunjukkan varietas dan aplikasi Daun Mindi, Sirsak dan Mahoni berpengaruh nyata pada keparahan penyakit busuk batang pada 5 dan 7 MST di MH 2015/2016. Pada musim hujan ditemukan yellowing pada varietas Inpari 25 dan Inpari 33. Akan tetapi, varietas Sintanur lebih tahan terhadap yellowing dengan nilai BWD pada dua musim tanam paling tinggi dibandingkan varietas lainnya. Sintanur memiliki keparahan penyakit Bercak Daun Cercospora paling rendah dibandingkan varietas lainnya. Keparahan penyakit umumnya meningkat seiring dengan bertambahnya umur padi, sedangkan kepadatan populasi hama dan musuh alami berfluktuasi. Kepadatan hama cukup tinggi pada plot kontrol, sedangkan kepadatan musuh alami cukup tinggi pada plot aplikasi ekstrak daun Mindi, Mahoni, dan Sirsak.Kata kunci: hama, musuh alami, penyakit, pertanian organik ABSTRACTOrganic rice cultivation intents to minimize the use of inorganic fertilizers and chemical pesticides, but in fact not apart from the disruption of pests and diseases. The study aimed to determine the pests, diseases, and natural enemies on organic rice cultivation. The research was conducted at Screen Field of ICRR at WS 2015/2016 and DS 2016, using Split Plot research design with three replications. The main plot was varieties of: 1). Inpari 25, 2). Inpari 33, and 3). Sintanur. The sub-plot was application of leaf extract of: 1). Mindi, 2). Soursop, 3).
ABSTRAKSeleksi Ketahanan Aksesi Plasma Nutfah Padi terhadap Hawar Daun Bakteri. Dini Yuliani, Rina H. Wening, dan Sudir. Usaha budi daya tanaman padi di Indonesia selalu dihadapkan pada berbagai kendala, di antaranya serangan penyakit hawar daun bakteri (HDB) yang disebabkan oleh bakteri Xanthomonas oryzae pv. oryzae (Xoo). Pengendalian penyakit HDB dengan varietas tahan merupakan salah satu teknik yang murah dan mudah dilakukan oleh petani padi. Aksesi plasma nutfah sebagai sumber tetua untuk perakitan varietas tahan perlu diketahui reaksi ketahanannya terhadap penyakit HDB. Penelitian ini bertujuan untuk menyeleksi ketahanan aksesi plasma nutfah terhadap penyakit HDB patotipe III, IV, dan VIII. Penelitian dilaksanakan di Kebun Percobaan Balai Besar Penelitian Tanaman Padi di Sukamandi, Subang, Jawa Barat pada Musim Hujan 2012/2013 dan Musim Kemarau 2013 dengan Rancangan Acak Terpisah. Petak utama adalah tiga patotipe Xoo, yaitu patotipe III, IV, dan VIII. Sedangkan anak petak adalah materi yang diuji, yaitu 20 aksesi plasma nutfah dan tiga varietas pembanding. Sebanyak 20 rumpun tanaman per petak diinokulasi Xoo dengan metode pengguntingan. Inokulasi dilakukan pada saat pertanaman menjelang stadium primordia. Ujung-ujung daun digunting sepanjang kira-kira 10 cm dari ujung daun dengan gunting inokulasi yang berisi suspensi bakteri Xoo umur 48 jam dengan kepekatan 10 8 cfu. Pengamatan keparahan penyakit HDB dilakukan dengan mengukur panjang gejala terpanjang pada umur dua, tiga, dan empat minggu setelah inokulasi. Hasil pengujian ketahanan terhadap HDB patotipe III, IV, dan VIII diperoleh tiga aksesi plasma nutfah yang berasal dari galur isogenik menunjukkan keparahan penyakit HDB tidak berbeda nyata dengan varietas pembanding tahan Angke pada dua musim tanam. Galur isogenik tersebut, yaitu IRBB 55, IRBB 60, dan IRBB 61. Ketiga galur isogenik tersebut dapat dijadikan tetua tahan dalam perakitan varietas unggul baru tahan HDB.Kata kunci: Seleksi ketahanan, aksesi plasma nutfah padi, hawar daun bakteri. ABSTRACTRice cultivation in Indonesia has been faced with many obstacles, including the attack of bacterial leaf blight (BLB) that caused by bacteria Xanthomonas oryzae pv. oryzae (Xoo).Resistant variety was considered as the cheap technique to control BLB disease and could be used by rice farmers. Germplasm accessions as a source to build resistant varieties must be known their resistance to BLB disease. This study aimed at selecting the resistance germplasm accessions to BLB pathotype III, IV, and VIII. Observations BLB disease severity was done by measuring the longest length of symptoms at the age of two, three, and four weeks after inoculation (WAI). The result showed that three germplasm accessions from near isogenic lines were not significantly different with the check varieties (Angke) in their resistance to Xoo pathotype III, IV, and VIII in two cropping seasons. The isogenic lines were IRBB 55, IRBB 60, and IRBB 61 can be used in the assembly of resistant new varieties to BLB.
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