Preliminary Evaluation of Paecilomyces lilacinus HY-4 to Control Tetranychus urticae

Abstract

The virulence against the two-spotted spider mite, Tetranychus urticae Koch, was evaluated with entomopathogenic fungus Paecilomyces lilacinus (Thom) Samson HY-4 which is isolated from coleopteran insect and registered to Korean and US patents. Virulence tests were conducted with conidial suspensions (1.5 ×10 6 conidia/ml) of P. lilacinus HY-4 against T. urticae adults and positive results were recorded in laboratory conditions. The spraying device was also developed for the efficient and exact evaluation of treatment. The developed spraying device was named as SD-tower sprayer and its efficacy of spraying conidia was evaluated. The accumulated mortality caused by P. lilacinus HY-4 using SD-tower spray was about 73% at 6 days after inoculation. This suggest that the isolate P. lilacinus HY-4 may be considered as promising for a new approach to prevent adult infestations by T. urticae.

Beauveria bassiana for the simultaneous control of Aedes albopictus and Culex pipiens mosquito adults shows high conidia persistence and productivity

“…Bioassays were conducted in a plastic cylinder (height 18, diameter 9 cm) capped with netting material. To evaluate the pathogenicity of the fungus, 10 mosquito adults aged 1–2 days were placed in the cylinder, and 1 mL of a conidial suspension (1 × 10 8 conidia/mL) was sprayed into each cylinder using an SD tower sprayer (Shin et al 2011). Cotton wool freshly soaked with a 10% sucrose solution was placed on the netting.…”

Entomopathogenic Fungi as Dual Control Agents against Both the Pest Myzus persicae and Phytopathogen Botrytis cinerea

“…Bioassays were conducted in 60-mm petri dishes containing a thin layer of 1.5% agar, and a 60 mm detached leaf disk of Chinese cabbage was placed on the plate. Twenty green peach aphid adults were placed on the leaf disks using a hair brush pen, and then 1 mL of suspensions containing each fungal culture filtrates, blastospores, conidia and their mixtures were sprayed into each dish by an SD tower sprayer [ 5 21 ]. Aphids were examined daily for mortality, and treatments were maintained at over 90% relative humidity in a chamber at 25 ± 1℃ for 7 days.…”

Screening and evaluation of entomopathogenic fungi against the green peach aphid, Myzus persicae, using multiple tools

“…Bioassays were conducted in 90-mm petri dishes containing a thin layer of 1.5% water agar, and a detached leaf disk of Chinese cabbage was placed on top. Twenty green peach aphid adults were placed onto leaf disks, and then 1 ml of a conidial suspension was sprayed into each dish using an SD tower sprayer (Shin et al, 2011), corresponding to a deposit of approximately in 5.8 ± 1.4 × 10 5 conidia/cm 2 leaf surface. Aphids were checked daily for mortality, and all cadavers were placed at N90% relative humidity in an incubator at 25°C until conidiation was observed.…”