Beauveria bassiana for the simultaneous control of Aedes albopictus and Culex pipiens mosquito adults shows high conidia persistence and productivity

Lee, Jin Yong; Woo, Ra Mi; Choi, Cheol Jun; Shin, Tae Young; Gwak, Won Seok; Woo, Soo Dong

doi:10.1186/s13568-019-0933-z

Cited by 21 publications

(27 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All previous studies of EPF targeting adult mosquitoes have focused on evaluating aqueous or oilformulated conidia, revealing differences in the efficacy of the formulations and virulence of different isolates of Beauveria or Metarhizium against, for example, Ae. aegypti [19], Aedes albopictus, Culex pipiens [20], Anopheles gambiae sensu stricto, Anopheles arabiensis, and Culex quinquefasciatus [21].…”

Section: Introductionmentioning

confidence: 99%

Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector

et al. 2021

View full text Add to dashboard Cite

Background The use of entomopathogenic fungi (EPF) for the control of adult mosquitoes is a promising alternative to synthetic insecticides. Previous studies have only evaluated conidiospores against adult mosquitoes. However, blastospores, which are highly virulent against mosquito larvae and pupae, could also be effective against adults. Methods Metarhizium anisopliae (ESALQ 818 and LEF 2000) blastospores and conidia were first tested against adult Aedes aegypti by spraying insects with spore suspensions. Blastospores were then tested using an indirect contact bioassay, exposing mosquitoes to fungus-impregnated cloths. Virulence when using blastospores suspended in 20% sunflower oil was also investigated. Results Female mosquitoes sprayed with blastospores or conidia at a concentration of 108 propagules ml−1 were highly susceptible to both types of spores, resulting in 100% mortality within 7 days. However, significant differences in virulence of the isolates and propagules became apparent at 107 spores ml−1, with ESALQ 818 blastospores being more virulent than LEF 2000 blastospores. ESALQ 818 blastospores were highly virulent when mosquitoes were exposed to black cotton cloths impregnated with blastospores shortly after preparing the suspensions, but virulence declined rapidly 12 h post-application. The addition of vegetable oil to blastospores helped maintain virulence for up to 48 h. Conclusion The results showed that blastospores were more virulent to adult female Ae. aegypti than conidia when sprayed onto the insects or applied to black cloths. Vegetable oil helped maintain blastospore virulence. The results show that blastospores have potential for use in integrated vector management, although new formulations and drying techniques need to be investigated. Graphical abstract

show abstract

Section: Introductionmentioning

confidence: 99%

Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Particular attention has been paid to fungal species belonging to the genera Lagenidium, Coelomomyces, Entomophthora, Culicinomyces, Beauveria, and Metarhizium for their power to reduce mosquito populations, but unfortunately, none of them have been specifically adapted as larvicidal agents against important vector species [109][110][111], even transgenic ones [112]. Application to surfaces on which mosquitoes land or need to pass through, such as fungus-impregnated cloths around bed nets, attractive bait stations, and adult mosquito traps and PET traps, show promising results, with a 39-50% reduction in survival rates of malaria-carrying mosquitoes and elimination of 95% of Anopheles arabiensis mosquitoes in a bait station [113].…”

Section: Fungimentioning

confidence: 99%

“…pipiens mosquito adults is Beauveria bassiana. The production and persistence of its conidia was remarkably high [109].…”

Section: Fungimentioning

confidence: 99%

Mosquito-Borne Diseases Emergence/Resurgence and How to Effectively Control It Biologically

2020

View full text Add to dashboard Cite

Deadly pathogens and parasites are transmitted by vectors and the mosquito is considered the most threatening vector in public health, transmitting these pathogens to humans and animals. We are currently witnessing the emergence/resurgence in new regions/populations of the most important mosquito-borne diseases, such as arboviruses and malaria. This resurgence may be the consequence of numerous complex parameters, but the major cause remains the mismanagement of insecticide use and the emergence of resistance. Biological control programmes have rendered promising results but several highly effective techniques, such as genetic manipulation, remain insufficiently considered as a control mechanism. Currently, new strategies based on attractive toxic sugar baits and new agents, such as Wolbachia and Asaia, are being intensively studied for potential use as alternatives to chemicals. Research into new insecticides, Insect Growth Regulators, and repellent compounds is pressing, and the improvement of biological strategies may provide key solutions to prevent outbreaks, decrease the danger to at-risk populations, and mitigate resistance.Pathogens 2020, 9, 310 2 of 26 are not used at all, and those that are still in the test or design phase but are very promising, which we suggest to be considered in the biological control of mosquito-borne diseases.

show abstract

“…Among the most employed natural biopesticides, there are microbial larvicides (e.g., Bacillus thuringiensis var. Israelensis or Wolbachia) [2] natural essential oils (e.g., oil of pennyroyal (Mentha pulegium) or Ruta chalepensis [3][4][5] entomopathogenic fungi, such as Metarhizium anisopliae or Beauveria bassiana (Bb) [6][7][8]. Precision pest management approaches, PPM [9,10], are diametrically opposite to random chemical spraying.…”

Section: Introductionmentioning

confidence: 99%

Proof of Concept of Biopolymer Based Hydrogels as Biomimetic Oviposition Substrate to Develop Tiger Mosquitoes (Aedes albopictus) Cost-Effective Lure and Kill Ovitraps

et al. 2022

View full text Add to dashboard Cite

Pest management is looking for green and cost-effective innovative solutions to control tiger mosquitoes and other pests. By using biomimetic principles and biocompatible/biodegradable biopolymers, it could be possible to develop a new approach based on substrates that selectively attract insects by reproducing specific natural environmental conditions and then kill them by hosting and delivering a natural biopesticide or through mechanical action (biomimetic lure and kill approach, BL&K). Such an approach can be theoretically specialized against tiger mosquitoes (BL&K-TM) by designing hydrogels to imitate the natural oviposition site’s conditions to employ them inside a lure and kill ovitraps as a biomimetic oviposition substrate. In this work, the hydrogels have been prepared to prove the concept. The study compares lab/on-field oviposition between standard substrates (absorbing paper/masonite) and a physical and chemically crosslinked hydrogel composition panel. Then the best performing is characterized to evaluate a correlation between the hydrogel’s properties and oviposition. Tests identify a 2-Hydroxyethylcellulose (HEC)-based physical hydrogel preparation as five times more attractive than the control in a lab oviposition assay. When employed on the field in a low-cost cardboard trap, the same substrate is seven times more capturing than a standard masonite ovitrap, with a duration four times longer.

show abstract

Beauveria bassiana for the simultaneous control of Aedes albopictus and Culex pipiens mosquito adults shows high conidia persistence and productivity

Cited by 21 publications

References 45 publications

Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector

Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector

Mosquito-Borne Diseases Emergence/Resurgence and How to Effectively Control It Biologically

Proof of Concept of Biopolymer Based Hydrogels as Biomimetic Oviposition Substrate to Develop Tiger Mosquitoes (Aedes albopictus) Cost-Effective Lure and Kill Ovitraps

Contact Info

Product

Resources

About