Preliminary evaluation of a thermosensitive chitosan hydrogel for Echinococcus granulosus vaccine delivery

Umair, Saleh; Pernthaner, A.; Deng, Qing; Gibson, Blake; Hook, Sarah; Heath, D.D.

doi:10.1016/j.vetpar.2017.02.007

Cited by 13 publications

(6 citation statements)

References 12 publications

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“…Its low toxicity and muco-adhesive properties made CS itself and its NPs good candidates for drug delivery systems. They act as penetration enhancers by opening the tight epithelial junctions (Pattani et al, 2009;Abdel-Latif et al, 2017;Mohammed et al, 2017;Umair et al, 2017). They proved better stability and cellular uptake than solid lipid nanoparticles (SLNs) (Wang et al, 2014;Luo et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Praziquantel Loaded Chitosan Nanoparticles Against Both Adult and Schistosomula Stages of Schistosoma Mansoni

Sadek

Harba

El-Refai

et al. 2018

Journal of the Egyptian Society of Parasitology

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Section: Introductionmentioning

confidence: 99%

Efficacy of Praziquantel Loaded Chitosan Nanoparticles Against Both Adult and Schistosomula Stages of Schistosoma Mansoni

Sadek

Harba

El-Refai

et al. 2018

Journal of the Egyptian Society of Parasitology

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“…This parasite infects body organs such as the liver and the lungs, causing potentially serious damage to human health (14). In order to ameliorate potential problems caused by the zoonotic parasite, development of an appropriate vaccine to prevent the on set of infection is vital (15)(16)(17).…”

Section: Discussionmentioning

confidence: 99%

Identification of combined T-cell and B-cell reactive Echinococcus granulosus 95 antigens for the potential development of a multi-epitope vaccine

Wang¹,

Gao²,

Lan³

et al. 2019

Ann. Transl. Med.

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Background: Identification of combined T-cell and B-cell reactive Eg95 antigens for the potential development of a multi-epitope vaccine against Echinococcus granulosus (EG), the causative agent of cystic echinococcosis (CE). Methods: This study involved the recombinant expression of Eg95 along with associated immune rabbit antiserum preparation. Bioinformatics technology was used to facilitate the analysis of Eg95 molecules. PCR was subsequently used to amplify genetic sequences of the epitopes encoding the T-cell and B-cell reactive peptide fragments. SDS-PAGE was used to assess the expression levels of three proteins. Eg95 serum and patient antiserum, which were assessed using Western blot in order to identify suitable antigenic epitope peptides. ELISA detection assay facilitated comparison of the immune reactivity of the short peptide epitopes. The assay results could be used to determine an EG epitope-based vaccine candidate list from suitably reactive Eg95 epitopes. Results: Eg95 molecules have 3 T-B table. The phage display systems were successfully built using the M13KE carrier. Expression of the three fusion protein peptides were detected. Western blot showed Eg95 antiserum against EG facilitated identification of the three T-cell and B-cell reactive epitopes. After the reaction intensities analyzed by the ELISA, both of the short peptide epitopes Eg95-2 and Eg95-3 showed strong signal strength and associated antigenicity when combined with patient serum and rabbit anti-rEg95 serum.Conclusions: This study used bioinformatics methods to construct successfully a T-cell and B-cell epitope phage display system for the Eg95 antigen from EG. The two epitopes of Eg95-2 and Eg95-3 demonstrated strong antigenicity with potential applications for peptide vaccine development.

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“…All antigens were expressed in a bacterial expression system (data unpublished). The eleven recombinant proteins were combined and dialysed overnight at 4 • C. As described previously [41], the antigens were formulated in the QuilA and chitin-based slow-release formulation. Each lamb was subcutaneously vaccinated with 75 µg of the vaccine on each vaccination.…”

Section: Animal Experiments and Collection Of Parasite Materialsmentioning

confidence: 99%

Multi-Omic Profiling, Structural Characterization, and Potent Inhibitor Screening of Evasion-Related Proteins of a Parasitic Nematode, Haemonchus contortus, Surviving Vaccine Treatment

et al. 2023

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The emergence of drug-resistant parasitic nematodes in both humans and livestock calls for development of alternative and cost-effective control strategies. Barbervax® is the only registered vaccine for the economically important ruminant strongylid Haemonchus contortus. In this study, we compared the microbiome, genome-wide diversity, and transcriptome of H. contortus adult male populations that survived vaccination with an experimental vaccine after inoculation in sheep. Our genome-wide SNP analysis revealed 16 putative candidate vaccine evasion genes. However, we did not identify any evidence for changes in microbial community profiling based on the 16S rRNA gene sequencing results of the vaccine-surviving parasite populations. A total of fifty-eight genes were identified as significantly differentially expressed, with six genes being long non-coding (lnc) RNAs and none being putative candidate SNP-associated genes. The genes that highly upregulated in surviving parasites from vaccinated animals were associated with GO terms belonging to predominantly molecular functions and a few biological processes that may have facilitated evasion or potentially lessened the effect of the vaccine. These included five targets: astacin (ASTL), carbonate dehydratase (CA2), phospholipase A2 (PLA2), glutamine synthetase (GLUL), and fatty acid-binding protein (FABP3). Our tertiary structure predictions and modelling analyses were used to perform in silico searches of all published and commercially available inhibitor molecules or substrate analogs with potential broad-spectrum efficacy against nematodes of human and veterinary importance.

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Preliminary evaluation of a thermosensitive chitosan hydrogel for Echinococcus granulosus vaccine delivery

Cited by 13 publications

References 12 publications

Efficacy of Praziquantel Loaded Chitosan Nanoparticles Against Both Adult and Schistosomula Stages of Schistosoma Mansoni

Efficacy of Praziquantel Loaded Chitosan Nanoparticles Against Both Adult and Schistosomula Stages of Schistosoma Mansoni

Identification of combined T-cell and B-cell reactive Echinococcus granulosus 95 antigens for the potential development of a multi-epitope vaccine

Multi-Omic Profiling, Structural Characterization, and Potent Inhibitor Screening of Evasion-Related Proteins of a Parasitic Nematode, Haemonchus contortus, Surviving Vaccine Treatment

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