Identification of combined T-cell and B-cell reactive Echinococcus granulosus 95 antigens for the potential development of a multi-epitope vaccine

Wang, Liang; Gao, Jian; Lan, Xi; Zhao, Hui; Shang, Xiaoqian; Tian, Fengming; Wen, Hao; Ding, Jianbing; Luo, Li; Ma, Xiumin

doi:10.21037/atm.2019.10.87

Cited by 13 publications

(7 citation statements)

References 20 publications

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“…External and internal antigens stimulate our immune system to secrete antibodies [61]. Cancer can express and release tumor-associated antigens into the circulating environment, so detection of their serum levels should assist in estimating the occurrence of malignancy, response to therapy and prognosis.…”

Section: Tumor-associated Circulating Materialsmentioning

confidence: 99%

Blood-based Markers in the Prognostic Prediction of Esophagogastric Junction Cancer

Liu¹,

Hong²,

Huang³

et al. 2020

J. Cancer

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Esophagogastric junction cancer poses a great threat to human beings both in western countries and East Asia, especially in China and Japan, and its incidence has increased during recent decades. The 5-year survival rate of esophagogastric junction cancer is quite poor compared with that of other gastric cancer sites. Until now, the traditional TNM staging system has been widely used in clinical practice for prognosis. However, the TNM system is based on pathology after surgical resection or radiology using CT and MRI, not on blood markers. Evidently, some research has been reported concentrated on the prognostic value of blood-based markers with the character of non-invasive and non-radioactive in EJA. Hematologic, biochemical and coagulation parameters could be obtained from clinical data and utilized to analyze their prognostic values. Tumor-associated antigens, microRNAs and circulating tumor cells have also been reported in EJC prognosis. In this article, we review research focused on blood-based markers to evaluate their prognostic value in esophagogastric junction cancer, especially its main subtype adenocarcinoma.

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Section: Tumor-associated Circulating Materialsmentioning

confidence: 99%

Blood-based Markers in the Prognostic Prediction of Esophagogastric Junction Cancer

Liu¹,

Hong²,

Huang³

et al. 2020

J. Cancer

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“…Therefore, it is difficult to diagnose, treat, and control [ 2 , 4 , 5 ]. Due to the limitations of current diagnostic and therapeutic measures, numerous studies have been carried out in the field of immunology associated with E. granulosus infection in the hope of developing vaccines [ 6 , 7 ]. Vaccination would be an effective preventive healthcare measure against echinococcosis [ 1 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

lncRNA028466 regulates Th1/Th2 cytokine expression and associates with Echinococcus granulosus antigen P29 immunity

et al. 2021

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Background Cystic echinococcosis (CE) is a parasitic disease that is caused by Echinococcus granulosus (Eg). The recombinant Echinococcus granulosus antigen P29 (rEg.P29) was shown to confer effective immunity to sheep and mice during E. granulosus secondary infection in our previous study. In this study, we sought to investigate the ability of long noncoding RNA 028466 (lncRNA028466) as a regulator for the protective immunity mediated by rEg.P29 vaccination and to study the effects of lncRNA028466 on CD4+T cell differentiation in mice spleen. Methods Female BALB/c mice were divided into two groups and were vaccinated subcutaneously with rEg.P29 antigen and PBS as a control (12 mice each group). Following prime-boost vaccination, CD4+T, CD8+T, and B cells from the spleen were isolated by flow cytometry. Quantitative real-time PCR (qRT-PCR) was performed to measure the expression of lncRNA028466 in these three kinds of cells. Then, lncRNA028466 was overexpressed and knocked down in naive CD4+T cells, and Th1 and Th2 cytokine expression was detected. qRT-PCR, western blot, and ELISA were performed to evaluate the production of IFN-γ, IL-2, IL-4, and IL-10, and flow cytometry was performed to detect the differentiation of Th1 and Th2 subgroups. Results lncRNA028466 was significantly decreased after the second week of immunization with rEg.P29 antigen. The proportion of CD4+ T cells was increased after rEg.P29 immunization. Overexpression of lncRNA028466 facilitated the production of IL-4, IL-10 and suppressed the production of IFN-γ, IL-2. Furthermore, after transfection with siRNA028466, IL-2 production was facilitated and IL-10 production was suppressed in naive CD4+ T cells. Conclusions Immunization with rEg.P29 downregulated the expression of lncRNA028466, which was related to a higher Th1 immune response and a lower Th2 immune response. Our results suggest that lncRNA028466 may be involved in rEg.P29-mediated immune response by regulating cytokine expression of Th1 and Th2.

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“… Not specified. [ 42 ] Female C57BL/6 and BALB/C mice (in-vivo model) Chimeric antigen made of 18 antigenic peptides from 5 different proteins (EgGST, EgA31, Eg95, EgTrp, P14-3-3). Protein obtained from the transformation of E.coli T-cell epitopes SC; 3 14 50 µg Freund’s adjuvant, complete and incomplete Measurement of mesenteric lymph nodes and spleen with a micrometer + ELISA (detection of cytokine levels) 99,6% of immunized mice with adjuvant were found to present no cysts in spleen, kidneys and liver.…”

Section: Next Generation Vaccinology For Ntdsmentioning

confidence: 99%

An Overview of Current Uses and Future Opportunities for Computer-Assisted Design of Vaccines for Neglected Tropical Diseases

Robleda-Castillo¹,

Ros-Lucas²,

Martínez-Peinado³

et al. 2021

AABC

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Neglected tropical diseases are infectious diseases that impose high morbidity and mortality rates over 1.5 billion people worldwide. Originally restricted to tropical and subtropical regions, changing climate conditions have increased their potential to emerge elsewhere. Control of their impact suffers from shortages like poor epidemiological surveillance or irregular drug distribution, and some NTDs still lack of appropriate diagnostics and/or efficient therapeutics. For these, availability of vaccines to prevent new infections, or the worsening of those already established, would mean a major breakthrough. However, only dengue and rabies count with approved vaccines at present. Herein, we review the state-of-the-art of vaccination strategies for NTDs, setting the focus on third generation vaccines and the concept of reverse vaccinology. Its capability to address pathogens´ biological complexity, likely contributing to save developmental costs is discussed. The use of computational tools is a fundamental aid to analyze increasingly large datasets aimed at designing vaccine candidates with the highest, possibly, opportunities to succeed. Ultimately, we identify and analyze those studies that took an in silico approach to find vaccine candidates, and experimentally assessed their immunogenicity and/or protection capabilities.

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Identification of combined T-cell and B-cell reactive Echinococcus granulosus 95 antigens for the potential development of a multi-epitope vaccine

Cited by 13 publications

References 20 publications

Blood-based Markers in the Prognostic Prediction of Esophagogastric Junction Cancer

Blood-based Markers in the Prognostic Prediction of Esophagogastric Junction Cancer

lncRNA028466 regulates Th1/Th2 cytokine expression and associates with Echinococcus granulosus antigen P29 immunity

An Overview of Current Uses and Future Opportunities for Computer-Assisted Design of Vaccines for Neglected Tropical Diseases

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