Preliminary analysis of different microRNA expression levels in juvenile angiofibromas

Abstract

Abstract. Juvenile angiofibroma (JA) is a rare fibrovascular tumor affecting, almost exclusively, adolescent males. The finding of frequent β-catenin-mutations in JAs emphasized the significance of the Wnt-signaling pathway in tumor pathogenesis. In the last decade, microRNAs (miRNAs or miRs) have been found to be involved in cancer pathogenesis by post-transcriptional regulation of gene expression and have not been analyzed in JAs thus far. In the present study, the expression of 4 miRNAs (hsa-let-7d, hsa-miR-98, hsa-miR-125a-5p and hsa-miR-218) was analyzed in 13 JAs and 3 deepithelized inferior nasal turbinates that were used as control tissue. The miRNA expression of hsa-let-7d (P= 0.158) and hsa-miR-98 (P= 0.069) was not statistically different between the two tissue types, however, a significant decrease in expression was observed for hsa-miR-125a-5p (P= 0.037) and hsa-miR-218 (P= 0.009) in JAs compared to inferior nasal turbinates. As downregulation of miRNA 218 has been recently shown to result in stabilization and nuclear accumulation of β-catenin, the present data indicates further evidence for the importance of the Wnt-signaling pathway in JAs.

Retracted: Epigenetic silenced miR‐125a‐5p could be self‐activated through targeting Suv39H1 in gastric cancer

“…miR‐125a‐5p has been previously reported to be down‐regulated in various human cancer types, including gastric cancer . Furthermore, miR‐125a‐5p has been validated to prevent the cancer cell progression . However, the underlying mechanism of the low expression of miR‐125a‐5p in gastric cancer remains unknown.…”

“…These data showed that overexpressing Furthermore, miR-125a-5p has been validated to prevent the cancer cell progression. [10][11][12][13][14][15][16][17][18][19]23,24 However, the underlying mechanism of the low expression of miR-125a-5p in gastric cancer remains unknown.…”

“…Among all the dysregulated miRNAs, miR-125a-5p is one of the most down-regulated or mutated miRNAs in gastric cancer tissues. 9 A number of studies have shown that the microRNA-125a-5p is an important tumour suppressor, and its expression is reduced in many types of human cancer, including in laryngeal cancer, 10 juvenile angiofibromas, 11 colorectal cancer, 12 breast cancer, 13,14 lung cancer, [15][16][17] cervical carcinomas, 18 prostate and pancreatic cancers. 19 MicroRNA-125a-5p has been demonstrated as an independent prognostic factor in gastric cancer and inhibiting the gastric cancer development, 20 through targeting oncogenes such as vascular endothelial growth factor A 21 and E2F transcription factor 3.…”

Current status and clinical association of beta-catenin with juvenile nasopharyngeal angiofibroma

“…It is possible that a beta-catenin mechanism operates independently of a familial adenomatous polyposis association or that the familial adenomatous polyposis in such cases remains subclinical. The novel finding of microRNA-218 down-regulation and its effect on beta-catenin further emphasises the significance of beta-catenin in tumour biology 21 …”

miR-218 impedes IL-6-induced prostate cancer cell proliferation and invasion via suppression of LGR4 expression

“…Additionally, IL-6 plays a specific role in the induction of LGR4 (11). however, miR-218 was found to inhibit AKT phosphorylation in oral cancer (33) and the downregulation of miR-218 was found to lead to stabilization and nuclear accumulation of β-catenin (34). Our results demonstrated that miR-218 targeted LGR4 by binding to its 3'-uTR, resulting in decreased LGR4 expression induced by IL-6 in the LNCaP-IL-6 + cells and downregulated the phosphorylation of PI3K/Akt and the accumulation of β-catenin.…”