Preischemic Administration of Nonexpanded Adipose Stromal Vascular Fraction Attenuates Acute Renal Ischemia/Reperfusion Injury and Fibrosis

Zhou, Liuhua; Xu, Luwei; Shen, Junda; Song, Qun; Wu, Rongqian; Ge, Yu‐Zheng; Hui, Xin; Zhu, Jun; Wu, Jianping; Jia, Ru

doi:10.5966/sctm.2015-0223

Cited by 40 publications

(61 citation statements)

References 69 publications

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“…Autologous SVF was isolated from epididymal adipose tissue according to our previously published protocol . Briefly, rat adipose tissue was washed thrice with ice‐cold sterile PBS, minced into small pieces, and digested with 0.075% type I collagenase at 37°C for 30‐40 minutes with gentle shaking.…”

Section: Methodsmentioning

confidence: 99%

“…Cell tracking of SVF was carried out by detecting CM‐DiI‐labeled cells according to our previous protocol . In brief, testicular tissues retrieved at 3 and 7 days after T/D were froze, cut into 5‐μm sections, and mounted with 4′‐6‐diamidino‐2‐phenylindole.…”

Section: Methodsmentioning

confidence: 99%

“…Adipose‐derived stromal vascular fraction (SVF) is a rich source of AdMSCs . Studies have reported that administration of uncultured SVF could attenuate injury and improve the function of various tissues and organs, such as the heart , kidney , urethral sphincter , etc. Recently, both our team and other groups have demonstrated that, compared with AdMSCs, uncultured SVF exhibited equal effect on attenuating acute renal I/R injury and recovering penile erection .…”

Section: Introductionmentioning

confidence: 99%

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Protective Effects of Uncultured Adipose-Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion-Detorsion in Rats

Zhou

Song

et al. 2018

Stem Cells Translational Medicine

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Torsion‐detorsion (T/D)‐induced testicular injury may lead to male subfertility and even infertility. Stem cell therapy provides an alternative to attenuate testicular injury and promote spermatogenesis. Adipose‐derived stromal vascular fraction (SVF) can be acquired conveniently without in vitro expansion, which may avoid the potential risks of microbial contamination, xenogenic nutritional sources, etc., during cell culture. In this study, we investigate the protective effects of autologous uncultured SVF on testicular injury and spermatogenesis in a rat model of T/D. Animals were randomly divided into sham, T/D+ phosphate‐buffered saline, and T/D + SVF groups (18 rats in each group). SVF was isolated, labeled with lipophilic fluorochrome chloromethylbenzamido dialkylcarbocyanine, and transplanted into T/D testis by local injection. At 3, 7, 14, and 28 days F surgery, testicular tissue and serum samples were harvested for histopathological, immunohistochemical, Western blot, and enzyme‐linked immunosorbent assays. Histopathological findings demonstrated severe injury in the testis with decreased Johnsen's score led by T/D, while uncultured SVF reduced testicular injury and elevated the decreased score. Injected SVF cells were mainly integrated into interstitial region and seminiferous tubules, enhanced the secretion of basic fibroblast growth factor and stem cell factor in the testis, contributed to the declining level of malondialdehyde and restoration of hormonal homeostasis, and then reduced the injury of Leydig cells and germ cells, as well as promoting spermatogenesis. Our findings demonstrated that autologous uncultured SVF could protect the testis from testicular ischemia‐reperfusion injury and promote spermatogenesis, which provide significant clinical implications for the prevention of infertility induced by testicular T/D. Stem Cells Translational Medicine 2019;8:383–391

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective Effects of Uncultured Adipose-Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion-Detorsion in Rats

Zhou

Song

et al. 2018

Stem Cells Translational Medicine

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“…In these etiologies, due to the high clinical rates of renal IR injury, especially in the perioperative period, such as with partial nephrectomy, kidney transplantation, and intraoperative hypotension, AKI has received more attention from scholars. Large amounts of evidence have indicated that IR‐induced AKI could cause laterenal fibrosis (Guo et al, ; Kim & Padanilam, ; Zhou et al, ), a common process for the progression of CKD to end‐stage renal disease (Nogueira, Pires, & Oliveira, ). Collagen I is a heterotrimer composed of two α1 and one α2 collagen peptide that plays an important regulatory role in the process of tissue fibrosis (Wada et al, ).…”

Section: Discussionmentioning

confidence: 99%

Curcumin alleviates ischemia reperfusion‐induced late kidney fibrosis through the APPL1/Akt signaling pathway

Chen

Fan

Huang

et al. 2018

Journal Cellular Physiology

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As a major cause of renal failure, transient renal ischemia and reperfusion induce both acute kidney injury and late fibrosis, which are the common pathological manifestations of end-stage renal disease. Curcumin is a biologically active polyphenolic compound found in turmeric. Increasing evidence has demonstrated that curcumin has a protective action against renal fibrosis, whereas mechanisms underlying the anti-fibrosis role of curcumin remain poorly defined. Here, we found that APPL1, an important intracellular binding partner for AdipoR, was involved in the pathogenesis of acute injury or fibrosis and was significantly upregulated by curcumin in a mouse model of ischemia reperfusion-induced late kidney fibrosis. Moreover, Akt signaling was the specific signaling pathway identified downstream of APPL1 in the pathogenesis of fibrosis. Our in vitro experiment demonstrated that curcumin alleviates ischemia reperfusion-induced late kidney fibrosis via the APPL1/Akt pathway. These data are helpful for understanding the anti-fibrosis mechanism of curcumin in the pathogenesis of AKI-induced late fibrosis.

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“…Many of these stem cell treatments involved a priori radiation and/or chemotherapy preparative regimens. In addition, the application of cellular therapies to other non-cancer indications, some of which are already licensed, include GvHD (33, 34), stroke (35), inflammatory bowel disease (36) lung disease (37), lupus (38), myocardial repair (39) and recovery from ischemia [e.g., limb (40, 41) and renal (42, 43)]. Although this list is by no means complete, information obtained from these studies can be leveraged to better understand the safety attributes of stem cells and how they could be used clinically for a radiation injury.…”

Section: Introductionmentioning

confidence: 99%

Cellular Therapies for Treatment of Radiation Injury: Report from a NIH/NIAID and IRSN Workshop

et al. 2017

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In recent years, there has been increasing concern over the possibility of a radiological or nuclear incident occurring somewhere in the world. Intelligence agencies frequently report that terrorist groups and rogue nations are seeking to obtain radiological or nuclear weapons of mass destruction. In addition, there exists the real possibility that safety of nuclear power reactors could be compromised by natural (such as the tsunami and subsequent Fukushima accident in Japan in March, 2011) or accidental (Three Mile Island, 1979 and Chernobyl, 1986) events. Although progress has been made by governments around the world to prepare for these events, including the stockpiling of radiation countermeasures, there are still challenges concerning care of patients injured during a radiation incident. Because the deleterious and pathological effects of radiation are so broad, it is desirable to identify medical countermeasures that can have a beneficial impact on several tissues and organ systems. Cellular therapies have the potential to impact recovery and tissue/organ regeneration for both early and late complications of radiation exposure. These therapies, which could include stem or blood progenitor cells, mesenchymal stromal cells (MSCs) or cells derived from other tissues (e.g., endothelium or placenta), have shown great promise in treating other nonradiation injuries to and diseases of the bone marrow, skin, gastrointestinal tract, brain, lung and heart. To explore the potential use of these therapies in the treatment of victims after acute radiation exposure, the National Institute of Allergy and Infectious Diseases cosponsored an international workshop in July, 2015 in Paris, France with the Institut de Radioprotection et de Sûreté Nucléaire. The workshop included discussions of data available from testing in preclinical models of radiation injury to different organs, logistics associated with the practical use of cellular therapies for a mass casualty incident, as well as international regulatory requirements for authorizing such drug products to be legally and readily used in such incidents. This report reviews the data presented, as well as key discussion points from the meeting.

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Preischemic Administration of Nonexpanded Adipose Stromal Vascular Fraction Attenuates Acute Renal Ischemia/Reperfusion Injury and Fibrosis

Cited by 40 publications

References 69 publications

Protective Effects of Uncultured Adipose-Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion-Detorsion in Rats

Protective Effects of Uncultured Adipose-Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion-Detorsion in Rats

Curcumin alleviates ischemia reperfusion‐induced late kidney fibrosis through the APPL1/Akt signaling pathway

Cellular Therapies for Treatment of Radiation Injury: Report from a NIH/NIAID and IRSN Workshop

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