Preimplantation human blastocysts release factors that differentially alter human endometrial epithelial cell adhesion and gene expression relative to IVF success

Cuman, Carly; Menkhorst, Ellen; Rombauts, Luk; Holden, S; Webster, David; Bilandzic, Maree; Osianlis, Tiki; Dimitriadis, Evdokia

doi:10.1093/humrep/det058

Cited by 47 publications

(48 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both ligands strongly localize to the endometrial glandular and luminal epithelium, with very little to no expression in the stroma. Jagged1 mRNA and protein are highest during the mid-secretory phase compared with the proliferative phase (Cobellis et al 2008, Cuman et al 2013. Whilst DLL4 mRNA expression is highest during the early secretory phase (Mazella et al 2008), DLL4 protein shows no significant changes across the cycle (Mitsuhashi et al 2012).…”

Section: Notch Signalling In the Endometriummentioning

confidence: 90%

“…To date, however, no studies have investigated the role of Notch in endometrial receptivity or blastocyst attachment in humans. However, there is reduced or absent immunostaining of Jagged1 in the mid-secretory luminal epithelium of women with primary infertility (Cuman et al 2013), suggesting that this ligand may be important for blastocyst-epithelial attachment. Furthermore, microarray analysis demonstrates that blastocyst-conditioned media regulates the endometrial epithelial expression of Notch1 and Jagged1 in vitro (Cuman et al 2013); thus, blastocysts may facilitate endometrial Notch expression, and possibly endometrial receptivity, via soluble mediators.…”

Section: Function Of Notch Signalling In Blastocyst-maternal Interactmentioning

confidence: 99%

“…1A; Mazella et al 2008, Cuman et al 2013 highlights the accessibility of Notch ligands and receptors for interactions during endometrial epithelial-trophectoderm attachment (Fig. 1A).…”

Section: Function Of Notch Signalling In Blastocyst-maternal Interactmentioning

confidence: 99%

See 2 more Smart Citations

Fetal–maternal communication: the role of Notch signalling in embryo implantation

Cuman¹,

Menkhorst²,

Winship³

et al. 2014

Reproduction

View full text Add to dashboard Cite

The establishment of a successful pregnancy requires the implantation of a competent blastocyst into a 'receptive' endometrium, facilitating the formation of a functional placenta. Inadequate or inappropriate implantation and placentation is a major reason for infertility and is thought to lead to first-trimester miscarriage, placental insufficiency and other obstetric complications. Blastocystendometrial interactions are critical for implantation and placental formation. The Notch signalling family is a receptor-ligand family that regulates cellular processes as diverse as proliferation, apoptosis, differentiation, invasion and adhesion. Notch signalling is achieved via cell-cell interaction; thus, via Notch, cells can have direct effects on the fate of their neighbours. Recently, a number of studies have identified Notch receptors and ligands in the endometrium, blastocyst and placenta. This review collates current knowledge of this large receptor-ligand family and explores the role of Notch signalling during implantation and placentation, drawing on information from both human and animal studies. Overall, the evidence suggests that Notch signalling is a critical component of fetal-maternal communication during implantation and placentation and that abnormal Notch expression is associated with impaired placentation and pre-eclampsia.Reproduction (2014) 147 R75-R86

show abstract

Section: Notch Signalling In the Endometriummentioning

confidence: 90%

Section: Function Of Notch Signalling In Blastocyst-maternal Interactmentioning

confidence: 99%

See 1 more Smart Citation

Fetal–maternal communication: the role of Notch signalling in embryo implantation

Cuman¹,

Menkhorst²,

Winship³

et al. 2014

Reproduction

View full text Add to dashboard Cite

show abstract

“…The profile of ECM proteins is remodelled and the expression of immune cells changes, giving rise to a priority of macrophages and uterine natural killer (NK) cells (13,14). When conception occurred and the blastocyst arrives into the uterus, it releases hormones, cytokines, integrins, growth factors, and other peptides that induce and facilitate endometrial receptivity (7,15,16). Embryo-endometrial interactions allow obtaining not only a receptive endometrium but also a competent blastocyst.…”

Section: Mechanisms Regulating the Embryo Endometrial Cross-talkmentioning

confidence: 99%

Periostin and implantation: a new biomarker of embryo-endometrial cross talk

Cello¹

2015

CCE

View full text Add to dashboard Cite

SummaryEmbryo-endometrium cross-talk represents the main limiting factor for a good implantation and pregnancy. At the time of implantation the interaction between blastocyst and endometrium, mediated by sex steroids hormones, immune cells and related soluble factors, induces significant modification in the superficial profile of extracellular matrix (ECM) proteins in both endometrium and blastocyst. More recently, it has been shown that also periostin (POSTN), a secreted ECM protein and a key element in metastatic processes plays an important role in the regulation of the embryo-endometrium cross-talk. It was observed that serum levels and tissue expression of POSTN may predict endometrial receptivity and pregnancy outcome implantation. Furthermore, ongoing data show a relationship between follicular fluid POSTN concentrations and embryo quality. Although a clinical validation it's required, preliminary results showed that, besides to the morphological, morphokinetic and genetic criteria, POSTN could be an easy non invasive biomarker to evaluate blastocyst quality and chance to obtain an adequate implantation. In this review we describe the main mechanisms by which POSTN may play its role in the regulation of embryo endometrium cross-talk.

show abstract

“…IL-6 is a well-established potent regulator of the host innate and humoral immune responses and has been shown to be up-regulated during a variety of viral infections including CMV [8,26,42]. IL-6 and its signalling molecules are also secreted by the endometrial and tubal cells of several species including human and have been described as major regulators of endometrial receptivity and blastocyst implantation [7,11,20]. Furthermore, abnormal expression of IL-6 by the endometrium has been associated with implantation failure, abortion and poor reproductive outcomes [13,20].…”

Section: Introductionmentioning

confidence: 99%

Aberrant expression of interleukin-6 and its receptor in Fallopian tubes bearing an ectopic pregnancy with and without tubal cytomegalovirus infection

Ashshi

2016

VirusDis.

View full text Add to dashboard Cite

Cytomegalovirus (CMV) has recently been suggested as a potential risk factor for the development of ectopic pregnancy (EP) following upper genital tract infection in women. However, little is known about its associated underlying pathogenic mechanisms. This was a prospective case-control study that measured the prevalence of CMV infection in Fallopian tubes (FT) bearing an EP and its effects on the tubal expression of interleukin (IL)-6 and its signaling molecules, which are known to play significant roles in the immune response against CMV infection as well as embryo implantation. Fresh FTs from 96 EPs during salpingectomy and another 61 women at the midluteal phase during total abdominal hysterectomy (TAH) were collected to measure the rate of CMV by an IVD CE PCR kit. The participants were then classified to measure the expression of IL-6, its receptor (IL6R) and intracellular mediators (gp-130, STAT3) by immunohistochemistry and quantitative RT-PCR. The results showed significantly higher (P = 0.01) rates of CMV in FTs obtained from EP (22.9 %) compared with controls (8.2 %). IL-6 (P = 0.003), IL6Ra (P = 0.02), gp 130 (P = 0.008) and STAT3 (P = 0.03) were significantly higher in TAH-positive (n = 5) compared with TAHnegative FTs by immunohistochemistry. Furthermore, the expression in the non-infected EP samples was significantly higher for IL-6 (P = 0.004), IL6R (P = 0.007), gp130 (P = 0.006) and STAT3 (P = 0.007) compared with negative TAH. Similar results were observed by quantitative PCR. CMV-positive EP samples showed the highest significant increase of the studied molecules by all techniques. In conclusion, Fallopian tubal infection with CMV is higher in EP and could predispose to embryo implantation by up-regulating the expression of IL-6 and its related molecules as part of tubal innate immune response. Further in vitro and in vivo studies are compulsory to illustrate the roles of IL-6 and CMV in the pathogenesis of EP.

show abstract

Preimplantation human blastocysts release factors that differentially alter human endometrial epithelial cell adhesion and gene expression relative to IVF success

Abstract: This study was supported by the National Health and Medical Research Council of Australia (Fellowship support #550905, #611827) and project grants by Monash IVF, Australia. There are no conflicts of interest to be declared.

Cited by 47 publications

References 33 publications

Fetal–maternal communication: the role of Notch signalling in embryo implantation

Fetal–maternal communication: the role of Notch signalling in embryo implantation

Periostin and implantation: a new biomarker of embryo-endometrial cross talk

Aberrant expression of interleukin-6 and its receptor in Fallopian tubes bearing an ectopic pregnancy with and without tubal cytomegalovirus infection

Contact Info

Product

Resources

About