In order to study the stability and dynamics of mountain rainforest and paramo ecosystems, including the biodiversity of these ecosystems, the Holocene and late Pleistocene climate and fire variability, and human impact in the southeastern Ecuadorian Andes, we present a high-resolution pollen record from El Tiro Pass (2810 m elevation), Podocarpus National Park. Palaeoenvironmental changes, investigated by pollen, spores and charcoal analysis, inferred from a 127 cm long core spanning the last ca. 21 000 cal. yr BP, indicate that grass-paramo was the main vegetation type at the El Tiro Pass during the late Pleistocene period. The grass-paramo was rich in Poaceae, Plantago rigida and Plantago australis, reflecting cold and moist climatic conditions. During the early Holocene, from 11 200 to 8900 cal. yr BP, subparamo and upper mountain rainforest vegetation expanded slightly, indicating a slow warming of climatic conditions during this period. From 8900 to 3300 cal. yr BP an upper mountain rainforest developed at the study site, indicated by an increase in Hedyosmun, Podocarpaceae, Myrsine and Ilex. This suggests a warmer climate than the present day at this elevation. The modern subparamo vegetation became established since 3300 cal. yr BP at El Tiro Pass. Fires, probably anthropogenic origin, were very rare during the late Pleistocene but became frequent after 8000 cal. yr BP.