Pregnancy‐Specific β₁Glycoprotein, SP₁ in Maternal Serum During Uncomplicated Single Pregnancies

Abstract

Pregnancy-specific beta 1-glycoprotein (SP1) levels in uncomplicated single pregnancies were measured by radioimmunoassay from the time of ovulation until the 8th week of pregnancy in 129 blood samples from 78 women. SP1 was detectable in all samples examined from 34 days after the last menstrual period (LMP), and thereafter the SP1 level increased rapidly with time and 38 to 40 days after LMP the geometric mean SP1 concentration was 90 micrograms/l. From pregnancy weeks 8 to 41, SP1 levels were measured by nephelometry in 1255 blood samples from 1255 women. A 95% reference range was established using logarithmic transformation. There was a steady increase in the SP1 concentration until the last month of pregnancy, in which a tendency to level off was seen, the geometric mean levels ranging from 149 to 170 mg/l. The day-to-day variation was studied during 5 consecutive days in 10 women. No significant variation was found. The diurnal variation was studied in blood samples taken every 4th hour during a 24-hour period form 10 women. A significant decrease was found at midnight and at 4 a.m. The elimination rate of SP1 from serum was studied in 10 women following labor. Elimination was non-linear and the time taken for SP1 to decrease to 50% ranged from 24 to 50 hours. No correlation was found between the concentration of SP1 and parity, maternal age, or the sex of the infant. The SP1 concentration was significantly lower in heavier women (greater than 70 kg) than in lighter women (less than or equal to 70 kg).(ABSTRACT TRUNCATED AT 250 WORDS)

Pregnancy-specific β1-glycoprotein (SP1) in serum from women with pregnancies complicated by intrauterine growth retardation

“…The reference range of serum SPj concentrations for uncomplicated single pregnancies used for comparison with the SP^ values obtained is the same as previously described [24].…”

“…The interassay variation was 5 % and the detection limit 0.5 mg/1. No differences were found between plasma and serum concentrations of SPi [24]. Whether the analyses were performed on plasma or serum, the term "serum concentration" will be used in the following.…”

“…Serum or plasma was stored at -20°C until assayed. Nephelometry was performed as described elsewhere [24]. The interassay variation was 5 % and the detection limit 0.5 mg/1.…”

“…levels in uncomplicated single pregnancies from a cross-sectional study [24] and with serial values from individual women with uncomplicated single pregnancies.…”

“…Pregnancy-specific ^-glycoprotein (SPj) levels measured by nephelometry in serum from 37 women (67 samples) with single pregnancies complicated by intrauterine growth retardation (infant birth weight below -2 SD of the normal mean for the gestational age) [7J. The shaded area represents a 95 % reference range for SPj levels in uncomplicated single pregnancies obtained from a cross-sectional study of 323 women [24], and the solid line is the geometric mean. The following symbols are used: APGAR score at 1 minute > 7 (·), idem < 6 (o), an infant with acrania and other malformations (Δ), an infant with ZELLWEGER's syndrome (A), an infant stillborn in pregnancy week 30 (+) and one stillborn in pregnancy week 35 (++).…”