Pregnancy Rate of Recipient Cows After Transfer of in Vitro-Produced Nellore Embryos

Jaguszeski, Mônica Zuchelli; Neto, Adalgiza Pinto; Oliveira, William de; Cattelam, Jonatas; Gregianini, Hélton Aparecido Garcia

doi:10.1590/1983-21252019v32n425rc

Cited by 6 publications

(5 citation statements)

References 7 publications

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“…It was also determined that the embryonic developmental stages had no effect on PR during fresh embryo transfer in beef heifers. In Zebu cattle,Zuchelli et al (2019) mentioned that ET at expanded or hatched blastocyst stage resulted in higher PR (47.35%) when compared to the ET at morula or initial blastocyst (41.06%) and at blastocyst stage (43%), which did not differ from each other. Hernandez-Fonseca et al (2023) reported higher PR in RF Brahman cross-bred heifers and cows transferred with hatched blastocysts when the ET was performed with different embryo stages (blastocyst, expanded blastocysts and hatched blastocysts).…”

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confidence: 90%

Evaluation of intrinsic and extrinsic factors affecting pregnancy rate in dual-purpose cows under tropical conditions

Zavaleta-Martínez,

Barrientos-Morales,

Medina

et al. 2024

Trop Anim Health Prod

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A total of 648 embryo transfer (ET) procedures were performed between January and December 2021 and were evaluated to assess the effect of these two types of factors on in-vitro produced ET programs carried out in tropical conditions. The intrinsic factors were size and location of the corpus luteum, body condition, genetic group, age and parity; while extrinsic factors were location of the farm, environmental comfort, season in which ET was carried out, prevailing weather conditions, and the preservation, quality, and development stage of embryos at the time of transfer. A χi 2 was used for analysis of main effects, and logistic regression analysis for the combined effects in each factor (intrinsic or extrinsic); additionally, a multivariate analysis of data clusters were used to nd a linkage between the effects. While age of the recipient female had a negative effect (Odds ratio = 0.179-0.870) on PR (p < 0.05), being higher in younger cows; the rest of the intrinsic factors did not affect (p > 0.05) the PR. The extrinsic factors signi cant (p < 0.05) were THI category, season of the year and type of embryo preservation during the ET, which indicates that the higher PR (p < 0.05) were obtained in the comfort THI category, winter season and the use of fresh semen during the ET. The clustering analysis did not show any linkage (p > 0.05) between PR and the intrinsic factors, while a linkage (p < 0.05) was found with yearly season and embryo preservation as extrinsic factors. It is concluded that age of the recipient cow and environmental conditions at the time of the transfer are key factors to be considered for successful invitro produced ET programs using dual-purpose cows under tropical conditions.

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confidence: 90%

Evaluation of intrinsic and extrinsic factors affecting pregnancy rate in dual-purpose cows under tropical conditions

Zavaleta-Martínez,

Barrientos-Morales,

Medina

et al. 2024

Trop Anim Health Prod

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“…In vivo, embryos in the early stage of development are allocated in the oviduct, which is an extremely important organ for embryo development ( Li and Winuthayanon, 2017 ) In vitro, embryos are normally produced in 2D culture systems, using culture dishes. Although IVP is a well-established technique, some limitations need improvements, such as lower blastocyst and birth rates ( Jaguszeski et al, 2019 ), and decreased resistance to cryopreservation ( Sanches et al, 2017 ) compared with in vivo -produced embryos.…”

Section: D Culture Applied To the Female Reproductive Systemmentioning

confidence: 99%

3D culture applied to reproduction in females: possibilities and perspectives

Ferronato,

Vit,

Silveira

2024

Anim. Reprod.

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In vitro cell culture is a well-established technique present in numerous laboratories in diverse areas. In reproduction, gametes, embryos, and reproductive tissues, such as the ovary and endometrium, can be cultured. These cultures are essential for embryo development studies, understanding signaling pathways, developing drugs for reproductive diseases, and in vitro embryo production (IVP). Although many culture systems are successful, they still have limitations to overcome. Three-dimensional (3D) culture systems can be close to physiological conditions, allowing greater interaction between cells and cells with the surrounding environment, maintenance of the cells' natural morphology, and expression of genes and proteins such as in vivo . Additionally, three-dimensional culture systems can stimulated extracellular matrix generating responses due to the mechanical force produced. Different techniques can be used to perform 3D culture systems, such as hydrogel matrix, hanging drop, low attachment surface, scaffold, levitation, liquid marble, and 3D printing. These systems demonstrate satisfactory results in follicle culture, allowing the culture from the pre-antral to antral phase, maintaining the follicular morphology, and increasing the development rates of embryos. Here, we review some of the different techniques of 3D culture systems and their applications to the culture of follicles and embryos, bringing new possibilities to the future of assisted reproduction.

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“…Andrade et al (2012) reportaram valores de taxa de prenhez de vacas submetidas à TE de 37,6% para vacas Senepol e 39,1% para vacas Nelore. Jaguszeski et al (2019) apresentaram valores maiores ainda, 45,15% de taxa de prenhez para vacas receptoras Nelore após a TE.…”

Section: Categoriaunclassified

Indução de gestação gemelar: uma ferramenta em prol da sustentabilidade na pecuária de corte

Bazzo,

Andreazzi,

Cavalieri

et al. 2023

FAAP

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A bovinocultura de corte se destaca no agronegócio nacional, contudo, as formas de produção devem buscar a sustentabilidade econômica e ambiental. Nessa busca, o emprego das biotecnologias da reprodução, como a indução de gestação gemelar, é necessário, pois pode aumentar a produção de carne por área. Assim, o objetivo desse estudo foi avaliar a técnica de indução de gestação gemelar em bovinos, por meio da transferência dupla de embriões. O estudo foi conduzido na Fazenda Unicesumar e foram utilizadas 100 receptoras bovinas, novilhas e vacas, sem raça definida, distribuídas em grupos: G1 – fêmeas inovuladas com 1 embrião e G2 – fêmeas inovuladas com 2 embriões. As receptoras foram sincronizadas e, no D17, inovuladas com 1 ou 2 embriões de Wagyu produzidos in vitro, de acordo com o grupo experimental. Foi avaliada a taxa de gestação aos 30 e aos 60 dias, taxa de parto, ocorrência de retenção de placenta e de parto distócico e o peso dos bezerros ao nascimento e aos 30 dias de idade. A inovulação dupla de embriões melhorou a taxa de prenhez aos 30 e aos 60 dias de gestação (p<0,05). As novilhas que receberam dois embriões se destacaram na taxa de prenhez aos 60 dias e na taxa de parto (p<0,05). Os bezerros oriundos das receptoras que receberam dois embriões nasceram mais leves (p<0,05). A indução de gestação gemelar em bovinos por meio transferência dupla de embriões é promissora e pode fomentar a produção de carne e contribuir com a sustentabilidade da pecuária de corte.

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Pregnancy Rate of Recipient Cows After Transfer of in Vitro-Produced Nellore Embryos

Cited by 6 publications

References 7 publications

Evaluation of intrinsic and extrinsic factors affecting pregnancy rate in dual-purpose cows under tropical conditions

Evaluation of intrinsic and extrinsic factors affecting pregnancy rate in dual-purpose cows under tropical conditions

3D culture applied to reproduction in females: possibilities and perspectives

Indução de gestação gemelar: uma ferramenta em prol da sustentabilidade na pecuária de corte

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