Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes

Alcala, Marco A.; Shade, Chad M.; Uh, Hyounsoo; Kwan, Shu Ying; Bischof, Matthias; Thompson, Zachary P.; Gogick, Kristy A.; Meier, Adam R.; Strein, Timothy G.; Bartlett, David L.; Modzelewski, Ruth A.; Petoud, Stéphane; Brown, Charles K.

doi:10.1016/j.biomaterials.2011.07.076

Cited by 34 publications

(34 citation statements)

References 32 publications

(28 reference statements)

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“…Their emission wavelengths are not affected by the environment, allowing them to be used in a broad range of conditions, including varied pH, and biological environments. Most luminescent lanthanide reporters are more resistant to photobleaching than organic fluorophores, which enables them to be used repeatedly and/or over long periods of time (7)(8)(9).…”

mentioning

confidence: 99%

Lanthanide near infrared imaging in living cells with Yb ³⁺ nano metal organic frameworks

Foucault‐Collet

Gogick

White

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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257

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We have created unique near-infrared (NIR)-emitting nanoscale metal-organic frameworks (nano-MOFs) incorporating a high density of Yb 3+ lanthanide cations and sensitizers derived from phenylene. We establish here that these nano-MOFs can be incorporated into living cells for NIR imaging. Specifically, we introduce bulk and nano-Yb-phenylenevinylenedicarboxylate-3 (nano-Yb-PVDC-3), a unique MOF based on a PVDC sensitizer-ligand and Yb 3+ NIRemitting lanthanide cations. This material has been structurally characterized, its stability in various media has been assessed, and its luminescent properties have been studied. We demonstrate that it is stable in certain specific biological media, does not photobleach, and has an IC 50 of 100 μg/mL, which is sufficient to allow live cell imaging. Confocal microscopy and inductively coupled plasma measurements reveal that nano-Yb-PVDC-3 can be internalized by cells with a cytoplasmic localization. Despite its relatively low quantum yield, nano-Yb-PVDC-3 emits a sufficient number of photons per unit volume to serve as a NIR-emitting reporter for imaging living HeLa and NIH 3T3 cells. NIR microscopy allows for highly efficient discrimination between the nano-MOF emission signal and the cellular autofluorescence arising from biological material. This work represents a demonstration of the possibility of using NIR lanthanide emission for biological imaging applications in living cells with single-photon excitation.uminescent reporters emitting in the near-infrared (NIR) region of the electromagnetic spectrum are highly advantageous for biological imaging applications for several reasons. Biological material has low autofluorescence in the NIR window, which allows facile discrimination between the desired signal of the reporter and the background, leading to an enhanced signalto-noise ratio and improved detection sensitivity (1). Additionally, NIR light scatters less than visible light, and therefore results in increased optical imaging resolution (2, 3). Finally, NIR photons interact less with biological material compared with visible photons, thus decreasing the risk of disturbing or damaging the biological systems being observed.NIR reporters, such as cyanine dyes (4, 5) and quantum dots (6), have previously been shown to be useful for biological imaging applications. However, these materials have broad emission bands that limit their ability to be easily discriminated from the background fluorescence. Additionally, cyanine dyes exhibit limited photostability and quantum dots can display blinking emission, making it difficult to conduct repeated or long-term experiments for such purposes as tracking a moiety or monitoring a process.Several lanthanide cations emit in the NIR and have some advantages with respect to organic fluorophores and semiconductor nanocrystals. Lanthanide cations have narrower emission bandwidths than organic fluorophores and semiconductor nanocrystals. Their emission wavelengths are not affected by the environment, allowing them to be used in a broad ra...

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mentioning

confidence: 99%

Lanthanide near infrared imaging in living cells with Yb ³⁺ nano metal organic frameworks

Foucault‐Collet

Gogick

White

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

257

154

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“…They have long luminescent lifetimes, which allows the removal of autofluorescence and improves the sensitivity through time‐resolved measurements . Luminescent lanthanide are more resistant to photobleaching, in relation to organic fluorophores, which enables them to be applied repeatedly and/or over long periods of time . Free lanthanide cations cannot generate the f→f transition because of poor extinction coefficients.…”

Section: Re Compoundsmentioning

confidence: 99%

“…[101] Luminescent lanthanide are more resistant to photobleaching, in relation to organic fluorophores,w hich enables them to be applied repeatedly and/or over long periodso ft ime. [102][103][104] Free lanthanide cations cannotg enerate the f!ft ransition because of poor extinction coefficients.T herefore, lanthanides require the use of ap hotonic converter,f or instance, an organic chromophore, through an "antenna converter". [105] The antennae need to be positioned close to the lanthanide to improvet he sensitivity,s o that the emitting compound possesses adequate photons for detection.…”

Section: Re Compoundsmentioning

confidence: 99%

Recent Progress in Fluorescence Imaging of the Near‐Infrared II Window

Miao

Zhu

et al. 2018

ChemBioChem

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Near‐infrared (NIR) fluorescent materials are considered to be the most promising labeling reagents for sensitive determination and biological imaging due to the advantages of lower background noise, deeper penetrating capacity, and less destructive effects on the biomatrix over those of UV and visible fluorophores. In the past decade, advances in biomedical fluorescence imaging in the NIR region have focused on the traditional NIR window (NIR‐I; λ=700–900 nm), and have recently been extended to the second NIR window (NIR‐II; λ=1000–1700 nm). In vivo NIR‐II fluorescence imaging outperforms imaging in the NIR‐I window as a result of further reduced absorption, tissue autofluorescence, and scattering. In this review, the applications of four types of NIR‐II fluorescent materials, organic fluorophores, quantum dots, rare‐earth compounds, and single‐walled carbon nanotubes, are summarized and future trends are discussed. Some methods to enhance the NIR‐II fluorescence quantum yield are also proposed.

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“…Dendrimers and dendrimer-based therapeutics are outstanding candidates in therapy and diagnostics field as more and more biological systems have benefited from these starburst molecules. Dendrimer conjugates have huge potential to serve as an innovative multifunctional delivery and imaging agent for the treatment and detection of metastatic tumors [79].…”

Section: Dendrimermentioning

confidence: 99%

EPR effect based nanocarriers targeting for treatment of cancer

Gajbhiye¹,

Gajbhiye²

2017

ijdd

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<p>The enhanced permeability and retention (EPR) effect is a unique phenomenon of solid tumors related to their anatomical and pathophysiological differences from normal tissues. In solid tumors, angiogenesis leads to high vascular density. Large gaps exist between endothelial cells in tumor blood vessels, which lead to selective extravasations and retention of macromolecular drugs. This EPR effect served as a basis for development of macromolecular anticancer therapy. There are various factors, which lead to a significantly increased EPR effect and enhanced antitumor drug effects as well. This review discusses the unique anatomy of tumor vessels, molecular mechanisms of factors related to the EPR effect and the role of the EPR effect in the intratumoral delivery of protein and peptide drugs, macromolecular drugs and drug-loaded long-circulating nanocarriers.</p>

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Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes

Cited by 34 publications

References 32 publications

Lanthanide near infrared imaging in living cells with Yb ³⁺ nano metal organic frameworks

Lanthanide near infrared imaging in living cells with Yb ³⁺ nano metal organic frameworks

Recent Progress in Fluorescence Imaging of the Near‐Infrared II Window

EPR effect based nanocarriers targeting for treatment of cancer

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Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes

Cited by 34 publications

References 32 publications

Lanthanide near infrared imaging in living cells with Yb 3+ nano metal organic frameworks

Lanthanide near infrared imaging in living cells with Yb 3+ nano metal organic frameworks

Recent Progress in Fluorescence Imaging of the Near‐Infrared II Window

EPR effect based nanocarriers targeting for treatment of cancer

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Lanthanide near infrared imaging in living cells with Yb ³⁺ nano metal organic frameworks

Lanthanide near infrared imaging in living cells with Yb ³⁺ nano metal organic frameworks