“…Indeed, the mapping of repetitive sequences in several families of this order, characterized by stable karyotypes, revealed a reduced phylogenetic variation (Motta-Neto et al, 2011a, b;Calado et al, 2013). On the other hand, heterochromatin heterogeneity has been reported in some fish groups with remarkable karyotypic changes such as Gobiidae (Caputo et al, 1997;Lima-Filho et al, 2014;Molina et al, 2014), Characidae (Mantovani et al, 2000), and Erythrinidae (Cioffi et al, 2009;Blanco et al, 2011). For instance, some species of marine Perciformes like Centropyge aurantonotus Burgess (1974) (Pomacanthidae), , and A. chirurgus (C) after Giemsa staining (left column), two-color FISH (middle column) with 18S rDNA (magenta signals), and 5S rDNA (green signals) probes and distribution of (TTAGGG)n sequences (right column).…”