Preface

Weissler, G. L.; Carlson, Rich

doi:10.1016/s0076-695x(08)60369-5

Cited by 9 publications

(9 citation statements)

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“…The XPS of commercially available MR showed, in addition to the expected peaks from carbon and oxygen, the presence of tin oxide; ‡ for M-CH 3 HL4 peaks attributed to Si 2p electrons were attributed to silicone oil contamination. 44 The XPS of the MR resin treated with Zn(OAc) 2 showed only a small amount of zinc (Fig. S13 †) and no indication of the presence of nitrogen.…”

Section: Nmr Spectroscopymentioning

confidence: 99%

Asymmetric zinc(ii) complexes as functional and structural models for phosphoesterases

et al. 2013

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We report two asymmetric ligands for the generation of structural and functional dinuclear metal complexes as phosphoesterase mimics. Two zinc(II) complexes, [Zn 2 (CH 3 L4)(CH 3 CO 2 ) 2 ] + (CH 3 HL4 = 2-(((2-methoxyethyl)( pyridin-2-ylmethyl)amino)methyl)-4-methyl-6-((( pyridin-2-ylmethyl)amino)methyl)-phenol) and [Zn 2 (CH 3 L5)(CH 3 CO 2 ) 2 ] + (CH 3 HL5 = 2-(((2-methoxyethyl)( pyridine-2-ylmethyl)amino)-methyl)-4-methyl-6-((( pyridin-2-ylmethyl)(4-vinylbenzyl)amino)methyl)phenol) were synthesized and characterized by X-ray crystallography. The structures showed that the ligands enforce a mixed 6,5-coordinate environment in the solid state. 1 H-, 13 C-and 31 P-NMR, mass spectrometry and infrared spectroscopy were used to further characterize the compounds in the solid state and in solution. The zinc (II) complexes hydrolyzed the organophosphate substrate bis-(2,4-dinitrophenol)phosphate (BDNPP), the nucleophile proposed to be a terminal water molecule ( pK a 7.2). The ligand CH 3 HL4 was immobilised on Merrifield resin and its zinc(II) complex generated. Infrared spectroscopy, microanalysis and XPS measurements confirmed successful immobilisation, with a catalyst loading of ∼1.45 mmol g −1 resin. The resin bound complex was active towards BDNPP and displayed similar pH dependence to the complex in solution.

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Section: Nmr Spectroscopymentioning

confidence: 99%

Asymmetric zinc(ii) complexes as functional and structural models for phosphoesterases

et al. 2013

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“…The silicon is proposed to be present from silicon oils used in the vacuum system of the XPS instrument [35]. A closer examination of the G3-MNP spectrum reveals that the core material is indeed Fe 3 O 4 [36,37].…”

Section: Characterization Of the Nanoparticlesmentioning

confidence: 99%

Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation

Daumann

Larrabee

Ollis

et al. 2014

Journal of Inorganic Biochemistry

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Annually thousands of people die or suffer from organophosphate (pesticide) poisoning. In order to remove these toxic compounds from the environment, the use of enzymes as bioremediators has been proposed. We report here a Ser127Ala mutant based on the enzyme glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes. The mutant, with improved metal binding abilities, has been immobilized using glutaraldehyde on PAMAM dendrimer-modified magnetite nanoparticles. The immobilized system was characterized using elemental analysis as well as infrared, transmission electron and X-ray photoelectron spectroscopies. The amount of GpdQ that was immobilized with the optimized procedure was 1.488 nmol per g MNP. A kinetic assay has been designed to evaluate the activity of the system towards organophosphoester substrates. The specific activity towards BPNPP directly after immobilization was 3.55 μmol mg -1 min -1 , after one week 3.39 μmol mg -1 min -1 and after 120 days 3.36 μmol mg -1 min -1 , demonstrating that the immobilized enzyme was active for multiple cycles and could be stored on the nanoparticles for a prolonged period.3

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“…Moreover Si 2p peaks are present in the immobilized ligand MR [31]. This is due to the fact that the resins were dried on a high vacuum oil pump and silicone oils are a known cause for this contamination [32]. Finally the XPS spectra of the immobilized complexes showed the two typical peaks of the Zn 2p electrons (Fig.…”

Section: Xps Of Mr and Immobilized Complexesmentioning

confidence: 99%

“…Also a contamination with silicon was detected. The latter is present only in the samples that had been dried with a high vacuum pump and thus it is concluded that this is the source of contamination [32]. Nonetheless the changes of the peak areas of the C, O, N and Zn peaks proved to be very useful to gain information about chemical environments and to confirm effective complex immobilization on the resin.…”

Section: Immobilization Of Biomimetics On Merrifield Resinmentioning

confidence: 99%

Immobilization of GpdQ and Related Models for Bioremedial Applications

Daumann

2014

Spectroscopic and Mechanistic Studies of Dinuclear Metallohydrolases and Their Biomimetic Complexes

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Preface

Cited by 9 publications

References 0 publications

Asymmetric zinc(ii) complexes as functional and structural models for phosphoesterases

Asymmetric zinc(ii) complexes as functional and structural models for phosphoesterases

Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation

Immobilization of GpdQ and Related Models for Bioremedial Applications

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