Preeclamptic women are deficient of interleukin-10 as assessed by cytokine release of trophoblast cells in vitro

Rein, Daniel T.; Breidenbach, Martina; Hönscheid, B.; Friebe-Hoffmann, U; Engel, Heike; Göhring, U.; Uekermann, L.; Kurbacher, Christian M.; Schöndorf, Thomas

doi:10.1016/s1043-4666(03)00220-5

Cited by 67 publications

(34 citation statements)

References 39 publications

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“…In PE patients, these vessels undergo minor modifications, with apparent substitution of the media layer cells by invasive trophoblasts, increased apoptosis of trophoblast cells, placental ischemia and loss of vasomotor control. Our results are consistent with the findings of other authors (Moll, Nienartowicz, Hees, Wrobel, & Lenz, 1988;Zhou, Damsky, & Fisher, 1997;Rein et al, 2003;Roberts & Gammill, 2005;Benirschke, Kaufmann, & Baergen, 2006).…”

Section: Immunohistochemistry (Ihc)supporting

confidence: 94%

<b>Expression of bradykinin in human placenta from healthy and preeclamptic women

Oliveira

Pereira

Bertevello

et al. 2017

Acta Sci. Health Sci.

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ABSTRACT. This study evaluated the expression of bradykinin (BK) in human placenta from healthy and preeclamptic women. This is a non-randomized experimental study, in which we performed histological analysis of placental tissue to observe changes that occur in each kind of placenta as well as immunohistochemical analysis to investigate the expression of bradykinin. We used 'Paleontological Statistics software package for education and data analysis' 3.06 and R for the statistical analysis. The Ethics Committee of the University of Rio Grande do Norte State approved this experiment under protocol number 166370, according to the determinations established by Resolutions 466/12 and 441/11.We found differences between the two kinds of placenta concerning the diameter of the vessels and the rate of cytotrophoblastic invasion. Student's t-test evidenced significant difference (p = 7.6395 x 10 -5 ) indicating greater marking of BK per section in the healthy placenta group. The result of more significant expression of bradykinin in healthy placenta can be used as a starting point for deeper researches aiming to better characterize and quantify this expression.Keywords: gynecology, obstetrics, immunohistochemistry.Expressão de bradicinina em placentas humanas saudáveis e pré-eclâmpticas RESUMO. Este estudo avaliou a expressão de bradicinina em placentas humanas saudáveis e pré-eclâmpticas. Trata-se de um estudo de caráter experimental não randomizado, no qual foi feita uma análise histológica dos tecidos placentários, permitindo a observação das alterações ocorridas em cada tipo de placenta, e a técnica de imuno-histoquímica, a fim de investigar a presença de bradicinina. Foi utilizado o Paleontological Statistics software package for education and data analysis 3.06 para a análise estatística. Este estudo foi aprovado pelo Comitê de Ética da Universidade do Estado do Rio Grande do Norte sob o protocolo número 166.370, de acordo com as diretrizes estabelecidas nas Resoluções 466/12 e 441/11. Foi possível observar diferenças no diâmetro dos vasos e na invasão citotrofoblástica entre os dois tipos de placentas. O teste t de Student mostrou significância estatística (p = 7,6395 x 10 -5 ) para uma maior marcação de BK por secção no grupo de placentas saudáveis. O resultado obtido com relação à expressão mais significante de bradicinina em placentas saudáveis pode ser visto como precursor de uma pesquisa mais aprofundada, para melhor caracterizar e quantificar essa expressão.Palavras-chave: ginecologia, obstetrícia, imuno-histoquímica.

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Section: Immunohistochemistry (Ihc)supporting

confidence: 94%

<b>Expression of bradykinin in human placenta from healthy and preeclamptic women

Oliveira

Pereira

Bertevello

et al. 2017

Acta Sci. Health Sci.

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“…IL-10 is directly involved in the repression of TNF-␣ release (14) and was recently shown to control the onset of irreversible septic shock after cecal ligation and puncture (19,42). IL-10 deficiency has been observed in a vast amount of inflammatory conditions including endotoxemia (9), inflammatory bowel disease (35), peritonitis (6), preeclampsia (25), and psoriasis (22), pointing to a role for IL-10 as a general control factor against inflammatory outburst.…”

mentioning

confidence: 99%

Effects of Forskolin on Kupffer Cell Production of Interleukin-10 and Tumor Necrosis Factor Alpha Differ from Those of Endogenous Adenylyl Cyclase Activators: Possible Role for Adenylyl Cyclase 9

et al. 2005

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(1 g/ml) caused attenuated TNF-␣ levels in culture medium (forskolin/isoproterenol, P < 0.05; prostaglandin E 2 , P < 0.01). Forskolin also reduced IL-10 mRNA and protein (P < 0.05), which was not observed with the other cAMPinducing agents. Furthermore, we found that rat Kupffer cells express high levels of the forskolin-insensitive adenylyl cyclase 9 compared to whole liver and that this expression is down-regulated by LPS (P < 0.05). We conclude that regulation of TNF-␣ and IL-10 in Kupffer cells depends on the mechanism by which cAMP is elevated. Forskolin and prostaglandin E 2 differ in their effects, which suggests a possible role of forskolininsensitive adenylyl cyclases like adenylyl cyclase 9.

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“…In cases of preeclampsia, there is an alteration in the balance between Th1 and Th2-type responses. By contrast, patients with preeclampsia have been shown to have higher levels of serum pro-inflammatory [15,16] and lower levels of anti-inflammatory cytokines [17] compared with healthy pregnant, thereby featuring a Th1-type predominance. Elevated levels of pro-inflammatory cytokines (TNF-α) or their inducers (IL-12) are also supported this activated Th1-type response [9,10,18].…”

Section: Discussionmentioning

confidence: 99%

Maternal Inflammatory Response in Severe Preeclamptic and Preeclamptic Pregnancies

Bayram¹

2012

J Clin Gynecol Obstet

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Preeclamptic women are deficient of interleukin-10 as assessed by cytokine release of trophoblast cells in vitro

Cited by 67 publications

References 39 publications

<b>Expression of bradykinin in human placenta from healthy and preeclamptic women

<b>Expression of bradykinin in human placenta from healthy and preeclamptic women

Effects of Forskolin on Kupffer Cell Production of Interleukin-10 and Tumor Necrosis Factor Alpha Differ from Those of Endogenous Adenylyl Cyclase Activators: Possible Role for Adenylyl Cyclase 9

Maternal Inflammatory Response in Severe Preeclamptic and Preeclamptic Pregnancies

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