Maternal Inflammatory Response in Severe Preeclamptic and Preeclamptic Pregnancies

Bayram,

doi:10.4021/jcgo44w

Cited by 5 publications

(7 citation statements)

References 28 publications

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“…31,32 Hence, our findings that hypoxic treatment of the dual placental perfusion system produces significantly higher levels of TNF-a and IFN-g closely parallel the in vivo response in preeclampsia. Furthermore, both IL-6 and IL-8 are found to be significantly increased in preeclampsia, 33,34 and both these pro-inflammatory cytokines were also secreted at a significantly increased rate under hypoxic treatment of the dual placental perfusion system compared with the normoxic group. As there are very few maternal lymphatic cells present in this system, this response to hypoxia most likely originates from the placental trophoblast.…”

Section: Discussionmentioning

confidence: 92%

Hypoxic treatment of human dual placental perfusion induces a preeclampsia-like inflammatory response

Jain

Schneider

Алиев

et al. 2014

Laboratory Investigation

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Preeclampsia is a human pregnancy-specific disorder characterized by a placental pro-inflammatory response in combination with an imbalance of angiogenic factors and clinical symptoms, including hypertension and proteinuria. Insufficient uteroplacental oxygenation in preeclampsia due to impaired trophoblast invasion during placentation is believed to be responsible for many of the molecular events leading to the clinical manifestations of this disease. We investigated the use of hypoxic treatment of the dual placental perfusion system as a model for preeclampsia. A modified perfusion technique allowed us to achieve a mean soluble oxygen tension within the intervillous space (IVS) of 5-7% for normoxia and o3% for hypoxia (as a model for preeclampsia). We assayed for the levels of different inflammatory cytokines, oxidative stress markers, as well as other factors, such as endothelin (ET)-1 that are known to be implicated as part of the inflammatory response in preeclampsia. Our results show a significant increase under hypoxia in the levels of different inflammatory cytokines, including IL-6 (P ¼ 0.002), IL-8 (Po0.0001), TNF-a (P ¼ 0.032) and IFN-g (P ¼ 0.009) at 360 min in maternal venous samples (n ¼ 6). There was also a significant increase in ET-1 levels under hypoxia both on the maternal side at 30 min (P ¼ 0.003) and fetal side at 360 min (P ¼ 0.036) (n ¼ 6). Other markers of oxidative stress, including malondialdehyde and 8-iso-protaglandin F2a (P ¼ 0.009) also show increased levels. Overall, these findings indicate that exposure of ex vivo dually perfused placental tissue to hypoxia provides a useful model for mimicking the inflammatory response characteristic of preeclampsia. This would therefore provide a powerful tool for studying and further delineating the molecular mechanisms involved in the underlying pathophysiology of preeclampsia.

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Section: Discussionmentioning

confidence: 92%

Hypoxic treatment of human dual placental perfusion induces a preeclampsia-like inflammatory response

Jain

Schneider

Алиев

et al. 2014

Laboratory Investigation

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“…Peningkatan sitokin pro-inflamasi juga diperlukan untuk merangsang produksi MMP-1, MMP-3, MMP-9 yang diperlukan untuk pematangan servik. 16 Pada akhir kehamilan normal juga terjadi peningkatan respon inflamasi yang berperan untuk persiapan persalian begitu juga yang terjadi pada preeklampsia tipe lambat. Pada wanita yang memiliki maternal konstitusi terjadi peningkatan ambang batas inflamasi, sehingga dengan adanya inflamasi kronik dengan intensitas rendah dapat menyebabkan timbulnya gejala preeklampsia.…”

Section: Interleukin-10 Pada Kehamilan Normalunclassified

“…Studi lain menunjukkan amplifikasi inflamasi pada preeklampsia oleh aktivasi sistem komplemen. 16,17 Teori inflamasi dan preeklampsia dari Redman dan Sargent membagi preeklampsia menjadi plasental dan maternal. Pada wanita normal, meskipun kehamilan normal dapat merangsang respon inflamasi sistemik, tetapi hal itu tidak cukup untuk menimbulkan pre-eklampsia.…”

Section: Interleukin-10 Pada Preeklampsiaunclassified

Perbedaan Kadar IL-10 pada Preeklampsia Tipe Dini dan Lambat

Danianto

Ernawati

2015

MOG

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Tujuan: Menganalisis perbedaan kadar IL-10 antara preeklampsia tipe dini dan preeklampsia tipe lambat. Bahan dan Metode: Penelitian ini melibatkan 56 wanita hamil yang terbagi menjadi empat kelompok, yaitu wanita hamil normal usia kehamilan 30-34 minggu, wanita hamil normal usia > 34 minggu, preeklampsia berat tipe dini (30-34 minggu), preeklampsia berat tipe lambat (> 34minggu). Pemeriksaan IL-10 dilakukan saat penderita masuk rumah sakit dan didiagnosis sebagai preeklampsia dengan menggunakan metode ELISA Hasil: Rerata kadar IL-10 pada kelompok preeklampsia tipe dini (0,37 ± 0,14) lebih rendah dibandingkan kelompok preeklampsia tipe lambat (0,87 ± 0,43) dengan P<0,001. Rerata kadar IL-10 pada kelompok preeklampsia tipe dini (0,37 ± 0,14) lebih rendah dibandingkan kelompok wanita hamil normal usia 30 -34 minggu (0,84 ± 0,42) dengan P<0,001. Tidak didapatkan perbedaan rerata kadar IL-10 pada kelompok preeklampsia tipe lambat (0,87 ± 0,43) dibandingkan kelompok wanita hamil normal usia > 34minggu (0,75 ± 0,68) P=0,22. Simpulan: Keadaan inflamasi berat terjadi pada preeklampsia berat tipe dini Kata Kunci : preeklampsia tipe dini, preeklampsia tipe lambat, IL-10Objectives: To analyze the difference of IL-10 concentration in early and late onset of preeclampsia. Materials and Methods: Cross-sectional analytic study was conducted on 56 pregnant women, divided into four groups : one groups of normal pregnant women with >30-34 weeks of gestation, one group of normal pregnant women with >34 weeks of gestation, one group of early onset preeclampsia (30-34 weeks), one group of late onset of preeclampsia (>34 weeks). Plasma IL-10 concentration examination was collected patient admitted in hospital and diagnosed preeclampsia. Plasma IL-10 was determined by ELISA. Results: Mean plasma IL-10 concentration on early onset preeclampsia group (0,37±0,14) was lower than late onset preeclampsia group (0,87 ± 0,43), (p<0,001). Mean plasma IL-10 concentration on early onset preeclampsia group (0,37 ±0,14) was lower than normal pregnant women with >30-34 weeks of gestation (0,84 ± 0,42), (p<0,001). Median plasma IL-10 concentration on late onset preeclampsia group (0,87 ±0,43) was higher than normal pregnant women with >34 weeks of gestation (0,75 ± 0,68), but not statistically significant (p=0,22). Conclusion: Severe inflammation happened in early onset preeclampsia.

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“…Complete failure of this mechanism would cause miscarriage, while partial failure would cause poor placentation and dysfunctional uteroplacental perfusion [8]. Bayram et al found that the patient with severe preeclampsia have higher values of IL-6 and IL-1β when compared to the normotensive ones [9]. Increased level of IL-6 and TNF-α may play a role of triggering factors for preeclampsia.…”

Section: Introductionmentioning

confidence: 99%

Prediction of the preeclampsia: a view of biochemical markers

Bostancı

Bayram

Cevrioğlu

et al. 2013

RHC

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Preeclampsia is a diverse, multiorgan group of related disease processes that occurs in up to 5%-8% of pregnancies after 20 weeks' gestation and it is one of the leading causes of maternal and fetal morbidity and mortality. Many molecular mechanisms are contributed to the pathogenesis of preeclampsia. Although it is unknown whether the mechanisms act independently or have synergistic effects. This review describes review of primary papers investigating blood based biomarker such as PAP-A, Inhibin A, sFlt1, and PP13 in general and first trimester biochemical markers and combinations of them specifically for preeclampsia.

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Maternal Inflammatory Response in Severe Preeclamptic and Preeclamptic Pregnancies

Cited by 5 publications

References 28 publications

Hypoxic treatment of human dual placental perfusion induces a preeclampsia-like inflammatory response

Hypoxic treatment of human dual placental perfusion induces a preeclampsia-like inflammatory response

Perbedaan Kadar IL-10 pada Preeklampsia Tipe Dini dan Lambat

Prediction of the preeclampsia: a view of biochemical markers

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