Predominance of CTX-M-15 among ESBL Producers from Environment and Fish Gut from the Shores of Lake Victoria in Mwanza, Tanzania

Moremi, Nyambura; Manda, Elizabeth V.; Falgenhauer, Linda; Ghosh, Hiren; Imirzalioglu, Can; Matee, Mecky; Chakraborty, Trinad; Mshana, Stephen E.

doi:10.3389/fmicb.2016.01862

Cited by 73 publications

(93 citation statements)

References 45 publications

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“…They all harboured bla CTX-M-15 located in the chromosome, at the same insertion sites with sequence type-ST-6359 indicating clonality. Recent studies involving isolates from animals and humans have shown the bla CTX-M-15 allele to be predominant in humans, fish, and animals, suggesting a circulation of this gene among different settings [34,35].…”

Section: Discussionmentioning

confidence: 99%

High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria

Ayandiran

Falgenhauer

Schmiedel

et al. 2018

J Infect Dev Ctries

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Introduction: Resistance to ciprofloxacin and tetracycline is increasing in the food chain especially in E. coli strains and more worrisome will be occurrence of extended-spectrum beta-lactamase (ESBL) producers among ciprofloxacin-and tetracycline-resistant isolates. This study was undertaken to investigate the occurrence and mechanism of ciprofloxacin-, tetracycline-and ESBL-resistant bacteria in poultry in Ibadan, Nigeria. Methodology: Bacteria were isolated from poultry feces in two farms in Ibadan and identified by MALDI-TOF. Antibiotic susceptibility patterns of the isolates were determined by disc diffusion and Minimum Inhibitory Concentration (MIC) using Vitek-2 apparatus. Four tetracycline genes and six plasmids mediated quinolone resistance genes (PMQR) were investigated by PCR. Whole genome sequencing was done for strains that were ESBL producers. Results: Bacterial strains (≥ 10 5 cfu/mL) were counted on ciprofloxacin and tetracycline supplemented plates. 106 bacteria from 14 different species were identified with high resistance to quinolones, tetracycline and trimethoprim. 49% of the strains were E. coli with 90% resistance for nalidixic acid, moxifloxacin (94%), ciprofloxacin (88%) levofloxacin (78%) and tetracycline (77%). The genes tetA, tetB, qnrB, qnrS and qepA were detected with 37%, 4%, 35%, 4% and 2% prevalence in E. coli respectively. Three ESBL-producing E. coli of the sequence type ST-6359 were found and harboured blaCTX-M-15 located in the chromosome, at the same insertion site. All the ESBL producers harboured mutations in gyrA (S83L/D87N/D678E) and parC (S80I). Conclusion: The observed high quinolones and tetracycline resistance with ESBL producers in this study calls for caution in the use of these antibiotics in poultry feeds.

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Section: Discussionmentioning

confidence: 99%

High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria

Ayandiran

Falgenhauer

Schmiedel

et al. 2018

J Infect Dev Ctries

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“…Apart from humans the environment as well as animals that are a food source serve as reservoirs for ESBLs and studies done on different continents have documented evidence, which in part explains the global presence and spread of ESBLs (1,2,12).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of multidrug-resistant Klebsiella pneumoniae and Escherichia coli harbouring extended spectrum beta-lactamases and carbapenemases genes at a tertiary hospital, Kenya

Maina¹,

Govind²,

Whitelaw³

2017

Microbiol Med

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SummaryBackground. Multidrug-resistant (MDR) Gram negative rods are increasingly being reported in sub-Saharan Africa. Molecular investigations play an important role, alongside other measures, in controlling nosocomial infections attributed to these organisms.This study aimed to determine the common extended spectrum beta-lactamases (ESBL) and carbapenemases genes, and clonal relationship in MDR Klebsiella pneumoniae and Escherichia coli.Methods. Fifty-four MDR isolates collected at the Aga Khan University hospital, Nairobi in the month of August 2012 formed the study. These were picked after an increase in the number of resistant strains during the said period was experienced.Results. blaCTXM was present in 41 (74%) of the isolates, while blaSHV was detected in 18 (33%) and blaTEM in 13 (24%) of the isolates.Nine (16.7%) of the isolates harboured all three ESBL genes and 8 (14.8%) harboured two. Eight of the isolates (all E. coli) had none of the ESBL genes tested. Two isolates harboured carbapenemases genotypes: one had blaNDM-1 and the other blaSPM.Sequencing matched CTXM-15 and TEM-1 genes in all the isolates harbouring blaCTXM and blaTEM respectively. However, there was diversity in blaSHV with SHV-11 and SHV-12 genes predominant. The isolates were non-clonal.Conclusions. The isolates mostly harboured blaCTX-M-15 while only a few had carbapenemases genes. Lack of clonality suggests these were the stable circulating strains at the time of the study.

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“…The reservoirs of ESBL-producing E. coli are warm-blooded animals including humans, which can transmit the bacteria to different environments, for example, aquatic environments, through fecal pollution (Jiang et al, 2012). Recently, studies have reported ESBL-producing E. coli in seafood such as shrimps, sardines, farmed fish, Nile tilapia (Oreochromis niloticus) as well as in frozen mackerel (Jiang et al, 2012;Moremi et al, 2016b;Nasreldin and Khaldoon, 2015;Dib et al, 2018). It has been documented that urban sewage, for example, from hospitals, is an important source of ESBL-producing enteric bacteria (Ojer-Usoz et al, 2013;Abgottspon et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In Tanzania, ESBL-producing E. coli have been isolated from different sources such as human specimens, foods, and aquatic environments (Moyo et al, 2010;Moremi et al, 2016b). Although ESBL-producing E. coli have been reported in humans in different regions of Tanzania (Moyo et al, 2010;Seni et al, 2016;Katakweba et al, 2018) and also in livestock and poultry (Katakweba et al, 2018), the importance of livestock and their meats as sources of ESBL-producing E. coli is unknown.…”

Section: Introductionmentioning

confidence: 99%

Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Aquatic Environment and Nile Perch (Lates niloticus) of Lake Victoria, Tanzania

et al. 2020

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Extended-spectrum β-lactamase (ESBL)-producing bacteria constitute an emerging global health issue with food products being vehicles of transmission and the aquatic environments serving as potential reservoirs. This study aimed to characterize ESBLproducing Escherichia coli in Nile perch and water from Lake Victoria in Tanzania. A total of 180 samples of Nile perch and 60 water samples were screened for ESBL-producing E. coli on MacConkey agar supplemented with 2 µg/ml of cefotaxime and confirmed by bla CTX−M and bla TEM PCR. Antimicrobial resistance was determined by the disk diffusion method, and the ESBL-producing isolates were whole genome sequencing (WGS). ESBL-producing E. coli were detected in eight of the 180 analyzed Nile perch samples, and only one water sample was positive (1.7%, n = 60). Isolates were resistant to sulfamethoxazole-trimethoprim (100%), ampicillin/cloxacillin (100%), erythromycin 72.7% (8/11), tetracycline 90.9% (10/11), and nalidixic acid 63.6% (7/11). This mostly corroborates the resistance genes that they carried for sulfonamides (sul1 and sul2), trimethoprim (dfrA and dfrB), aminoglycosides [aac(3)-IId, strA, and strB], tetracycline [tet(B) and tet(D)], and fluoroquinolones (qepA4). They harbored plasmid replicon types IncF, IncX, IncQ, and Col and carried bla CTX−M−15 and bla TEM−1B genes generally found on the same contigs as the IncF plasmid replicon. Although epidemiologically unrelated, the strains formed three separate sequence type-phylogroup-serotypespecific clusters: C1, C2, and C3. Cluster C1 included five strains (3 to 13 SNPs) belonging to ST167, phylogroup A, and serotype O9:H21; the two C2 strains (11 SNPs) belong to ST156, phylogroup B1, and serotype ONT:H28; and C3 was made up of four strains (SNPs ranged from 4 to 17) of ST636, phylogroup B2, and serotype O45:H7. The common virulence gene gad was reported in all strains. In addition, strains in C2 and C3 possessed iss, lpfA, and nfaE virulence genes, and the vat gene was found only in C3. The present study reports the occurrence of multidrug-resistant ESBL-producing Frontiers in Microbiology | www.frontiersin.org February 2020 | Volume 11 | Article 108 Baniga et al.Genomics of ESBL-E. coli in Lake Victoria E. coli carrying plasmid-mediated ESBL genes in offshore water and Nile perch in Lake Victoria. Strains formed three clonal clusters of unknown origin. This study reveals that the Lake may serve as reservoir for ESBL-producing bacteria that can be transmitted by fish as a food chain hazard of One-Health concern.

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Predominance of CTX-M-15 among ESBL Producers from Environment and Fish Gut from the Shores of Lake Victoria in Mwanza, Tanzania

Cited by 73 publications

References 45 publications

High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria

High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria

Molecular characterization of multidrug-resistant Klebsiella pneumoniae and Escherichia coli harbouring extended spectrum beta-lactamases and carbapenemases genes at a tertiary hospital, Kenya

Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Aquatic Environment and Nile Perch (Lates niloticus) of Lake Victoria, Tanzania

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