Predominance of certain klebsiella capsular typesin hospitals in the United Kingdom

Casewell, M.W.; Talsania, H.

doi:10.1016/s0163-4453(79)80057-2

Cited by 39 publications

(22 citation statements)

References 7 publications

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“…This is in agreement with other studies, which show this K antigen to be very common among clinical Klebsiella isolates [25][26][27]. In K. planticola by contrast, K14 was the predominant capsular type (13%).…”

Section: Discussionsupporting

confidence: 93%

Expression of putative virulence factors by clinical isolates of Klebsiella planticola

Podschun

Fischer

Ullman

2000

Journal of Medical Microbiology

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A total of 92 clinical isolates of Klebsiella planticola from man was examined with respect to the production of haemagglutinins and siderophores, serum resistance and distribution of capsular types. For comparison, a group of 207 clinical isolates of K. pneumoniae was also studied. The percentages of K. planticola strains able to express mannose-sensitive haemagglutination, indicating type 1 fimbriae (83%) and mannose-resistant and Klebsiella-like agglutination, indicating type 3 fimbriae (69%), as well as to produce the siderophores enterobactin (100%) and aerobactin (2.2%) were almost identical to those of the K. pneumoniae strains. Similarly, the proportion of serum-resistant strains (30%) was comparable to that of K. pneumoniae (25%). The capsule types most often detected in K. planticola were K14 (13%), K2 (9%) and K70 (9%). The incidence of K2, which is the predominant capsular type in K. pneumoniae, was similar in both species. These findings show that K. planticola, which is being detected with increasing frequency in clinical specimens from man, has the ability to express similar putative virulence factors to K. pneumoniae, suggesting that they may have similar pathogenicity.

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Section: Discussionsupporting

confidence: 93%

Expression of putative virulence factors by clinical isolates of Klebsiella planticola

Podschun

Fischer

Ullman

2000

Journal of Medical Microbiology

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“…A polysaccharide-based vaccine has been tested (5). Based on the structural variability of its capsular polysaccharides (CPS), Klebsiella has been classified into 77 serotypes (27), which differ markedly in pathogenicity potential and epidemiological relevance (3,28,29,35). Epidemiological findings showed that over 70% of all cases of Klebsiella bacteremia were caused by only 25 of the 77 different serotypes (6).…”

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confidence: 99%

Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae

Podschun²,

et al. 2000

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The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistant Klebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization of Klebsiella.Klebsiella pneumoniae is an opportunistic pathogen involved in outbreaks of nosocomial infections, such as bacteremia and sepsis, mainly in immunocompromised individuals (36). Due to the emergence of multidrug resistance among Klebsiella strains, the search for new approaches for the prevention or treatment of Klebsiella infections is now under intensive investigation (34). To be successful, these efforts will require a better understanding of the infectious process.Multiple Klebsiella components (e.g., fimbriae, siderophores, O antigens, and capsular antigens) have been considered to be potential virulence factors (34). Of these factors, capsular antigens are probably considered the major determinants of pathogenicity (4,9,17,21). As a consequence, new therapeutic approaches have been targeted against the capsule. A polysaccharide-based vaccine has been tested (5). Based on the structural variability of its capsular polysaccharides (CPS), Klebsiella has been classified into 77 serotypes (27), which differ markedly in pathogenicity potential and epidemiological relevance (3,28,29,35). Epidemiological findings showed that over 70% of all cases of Klebsiella bacteremia were caused by only 25 of the 77 different serotypes (6).

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“…The range of capsular types each vaccine protected against was however unpredictable. For future preparations of vaccine it would be worth searching for bacteria that yield vaccines giving a broad spectrum of protective cover, especially as epidemiological studies show a wide range of klebsiella capsular types appearing in clinical isolates (Casewell & Talsania, 1979;Riser & Noon, 1981).…”

Section: Discussionmentioning

confidence: 99%

Vaccination againstKlebsiella aerogenes

Roe¹,

Jones²

1984

J. Hyg.

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SummaryKlebsiella vaccine was prepared from strains ofKlebsiella aerogeneswith capsular types Kl, K36, K44 and K Cross (a type which cross-reactsin vitrowith sera from many klebsiella capsular types). The vaccine was extracted by dialysis and ultrafiltration from capsular material released during growth of the bacteria in a five-day batch culture.Mice given one dose of vaccine from K1a (l·0 μg/mouse) survived lethal intraperitoneal challenge of 11/11 homologous klebsiella strains four days after vaccination; 14 days after vaccination protection against the same challenge strains had declined to 5/11 strains. Vaccines from Kla, b, c, K3G, K44 and K Cross induced homologous protection and protected mice against different ranges of heterologous klebsiella capsular types.The protective response of the mice was greatly enhanced by administering three doses of the vaccines. Vaccines from Kl, K36, K44 and K Cross protected mice against 14/20, 11/20, 10/20 and 9/20 homologous and heterologous klebsiella challenge strains respectively.None of the klebsiella vaccines was toxic for mice at the immunizing dose (l·0 μg/mouse). Vaccine from K36 was the most lethal, killing mice at 103immunizing doses. The least toxic vaccine was from K44, which killed mice at 104immunizing doses.

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Predominance of certain klebsiella capsular typesin hospitals in the United Kingdom

Cited by 39 publications

References 7 publications

Expression of putative virulence factors by clinical isolates of Klebsiella planticola

Expression of putative virulence factors by clinical isolates of Klebsiella planticola

Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae

Vaccination againstKlebsiella aerogenes

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