Predominance of Central Asian and European families among Mycobacterium tuberculosis isolates in Kashmir Valley, India

Bashir, Gulnaz; Wani, Tehmeena; Sharma, Pragya; Katoch, Vishwa Mohan; Lone, Rubina; Shah, Azra; Katoch, Kiran; Kakru, Dalip K; Chauhan, D. S.

doi:10.1016/j.ijtb.2017.05.004

Cited by 3 publications

(3 citation statements)

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“…Arora et al [ 18 ] reported SIT1/Beijing and SIT26/CAS1-Delhi as predominant genotype among the XDR-TB isolates in and around the Delhi region of India. The predominance of CAS1-Delhi and Beijing strains among MDR-TB patients in this study and presence of these clades in other parts of the country [ 5 , 12 , 13 , 24 ] underlines the rapid dissemination of these lineages across large geographical regions in India. In present study, Beijing clade is followed by ill-defined Manu (5.11%), EAI3_IND clade (4.09%), T (4.09%), CAS (3.41%), CAS2 (0.68%), LAM (0.68%), X (0.68%) and Haarlem (0.34%) in decreasing order.…”

Section: Discussionmentioning

confidence: 70%

“…Despite the largest number of MDR-TB patients (99,000 cases; 27% of global cases), less is known about the genetic biodiversity of MDR- M.tuberculosis strains in India. Most of the studies done so far either explored only the circulating genotypes of M. tuberculosis or included less number of MDR- M.tuberculosis isolates in the country [ 3 – 6 , 12 , 13 ]. To our knowledge, present study includes largest number of clinical MDR M.tuberculosis isolates ( n = 293) to describe the predominant lineages and sub-lineages circulating in pulmonary MDR-TB patients in North India.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Desikan et al, [ 15 ] reported the predominance of SIT26/CAS1_Del strains in central India. Basir et al, [ 13 ] also reported the predominance of SIT26/ CAS1_Del in isolates from Kashmir Valley, India. Arora et al [ 18 ] reported SIT1/Beijing and SIT26/CAS1-Delhi as predominant genotype among the XDR-TB isolates in and around the Delhi region of India.…”

Section: Discussionmentioning

confidence: 99%

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Genetic variability in multidrug-resistant Mycobacterium tuberculosis isolates from patients with pulmonary tuberculosis in North India

Singh

Yadav

et al. 2021

BMC Microbiol

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Background Information on the genetic variability of drug resistant isolates of Mycobacterium tuberculosis is of paramount importance to understand transmission dynamics of disease and to improve TB control strategies. Despite of largest number of multidrug-resistant (MDR) tuberculosis cases (1, 30,000; 27% of the global burden), strains responsible for the expansion or development of drug-resistant Mycobacterium tuberculosis infections have been poorly characterized in India. Present study was aimed to investigate the genetic diversity in MDR isolates of Mycobacterium tuberculosis in North India. Results Spacer oligonucleotide typing (spoligotyping) was performed on 293 clinical MDR isolates of Mycobacterium tuberculosis recovered from cases of pulmonary tuberculosis from North India. Spoligotyping identified 74 distinct spoligotype patterns. Comparison with an international spoligotype database (spoldb4 database) showed that 240 (81.91%) and 32 (10.92%) strains displayed known and shared type patterns, while 21 (7.16%) strains displayed unique spoligotype patterns. Among the phylogeographic lineages, lineage 3 (East African-Indian) was found most predominant lineage (n = 159, 66.25%), followed by lineage 2 (East Asian; n = 34, 14.16%), lineage 1 (Indo-Oceanic; n = 30, 12.50%) and lineage 4 (Euro American; n = 17, 7.08%). Overall, CAS1_DEL (60.41%; SITs 2585, 26, 2694, 309, 381, 428, 1401, 141, 25, 1327) was found most pre-dominant spoligotype pattern followed by Beijing (14.16%; SITs255, 260, 1941, 269) and EAI3_IND (5.00%; SITs 298, 338, 11). The demographic and clinical characteristics were not found significantly associated with genotypic lineages of MDR-M.tuberculosis isolates recovered from pulmonary TB patients of North India. Conclusions Present study reveals high genetic diversity among the Mycobacterium tuberculosis isolates and highlights that SIT141/CAS1_Del followed by SIT26/ Beijing lineage is the most common spoligotype responsible for the development and transmission of MDR-TB in North India. The high presence of shared type and unique spoligotype patterns of MDR strains indicates epidemiological significance of locally evolved strains in ongoing transmission of MDR-TB within this community which needs to be further monitored using robust molecular tools with high discriminatory power.

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