Predominance of a macrolide-lincosamide-resistant Brachyspira hyodysenteriae of sequence type 196 in Swiss pig herds

García-Martín, Ana B.; Perreten, Vincent; Rossano, Alexandra; Schmitt, Sarah; Zeeh, Friederike

doi:10.1016/j.vetmic.2018.10.007

Cited by 12 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The major acquired extended-spectrum β-lactamase (Dallenne et al, 2010; Hussain et al, 2014; Ranjan et al, 2017), fosfomycin (Dantas Palmeira et al, 2018), chloramphenicol (White et al, 1999), lincosamide (Garcia-Martin et al, 2018), and carbapenemase genes (Chen et al, 2015; Ranjan et al, 2016, 2017) were detected by PCR, after which all the PCR amplicons were sequenced on the ABI 3730 platform (Applied Biosystems, United States). The sodium azide-resistant E. coli J53Azi R strain was used as the recipient and the P. mirabilis HFK418 isolate as the donor for the conjugative transfer of the plasmids.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a NDM-1- Encoding Plasmid pHFK418-NDM From a Clinical Proteus mirabilis Isolate Harboring Two Novel Transposons, Tn6624 and Tn6625

Dong

Liu

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

Acquisition of the blaNDM–1 gene by Proteus mirabilis is a concern because it already has intrinsic resistance to polymyxin E and tigecycline antibiotics. Here, we describe a P. mirabilis isolate that carries a pPrY2001-like plasmid (pHFK418-NDM) containing a blaNDM–1 gene. The pPrY2001-like plasmid, pHFK418-NDM, was first reported in China. The pHFK418-NDM plasmid was sequenced using a hybrid approach based on Illumina and MinION platforms. The sequence of pHFK418-NDM was compared with those of the six other pPrY2001-like plasmids deposited in GenBank. We found that the multidrug-resistance encoding region of pHFK418-NDM contains ΔTn10 and a novel transposon Tn6625. Tn6625 consists of ΔTn1696, Tn6260, In251, ΔTn125 (carrying blaNDM–1), ΔTn2670, and a novel mph(E)-harboring transposon Tn6624. In251 was first identified in a clinical isolate, suggesting that it has been transferred efficiently from environmental organisms to clinical isolates. Genomic comparisons of all these pPrY2001-like plasmids showed that their relatively conserved backbones could integrate the numerous and various accessory modules carrying multifarious antibiotic resistance genes. Our results provide a greater depth of insight into the horizontal transfer of resistance genes and add interpretive value to the genomic diversity and evolution of pPrY2001-like plasmids.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of a NDM-1- Encoding Plasmid pHFK418-NDM From a Clinical Proteus mirabilis Isolate Harboring Two Novel Transposons, Tn6624 and Tn6625

Dong

Liu

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…According to the Italian situation, in the majority of European studies that investigated the resistance to tylosin, lincomycin, doxycycline, and tiamulin in the last decade, it is evident that the reduction in sensitivity to both tylosin and lincomycin is common and frequently concomitant. On the other hand, resistance to tiamulin and/or doxycycline is not always observed among European strains, although, when present, it covers a high percentage of the tested strains [23,31,[33][34][35][36]. This can be explained by a selective environmental pressure that results in an increasing multidrug resistance, as happens in our field strain.…”

Section: Discussionmentioning

confidence: 61%

Dual Antimicrobial Effect of Medium-Chain Fatty Acids against an Italian Multidrug Resistant Brachyspira hyodysenteriae Strain

Giovagnoni

Tugnoli²,

Piva

et al. 2022

Microorganisms

View full text Add to dashboard Cite

The fastidious nature of Brachyspira hyodysenteriae limits an accurate in vitro pre-screening of conventionally used antibiotics and other candidate alternative antimicrobials. This results in a non-judicious use of antibiotics, leading to an exponential increase of the antibiotic resistance issue and a slowdown in the research for new molecules that might stop this serious phenomenon. In this study we tested four antibiotics (tylosin, lincomycin, doxycycline, and tiamulin) and medium-chain fatty acids (MCFA; hexanoic, octanoic, decanoic, and dodecanoic acid) against an Italian field strain of B. hyodysenteriae and the ATCC 27164 strain as reference. We determined the minimal inhibitory concentrations of these substances, underlining the multidrug resistance pattern of the field strain and, on the contrary, a consistent and stable inhibitory effect of the tested MCFA against both strains. Then, sub-inhibitory concentrations of antibiotics and MCFA were examined in modulating a panel of B. hyodysenteriae virulence genes (tlyA, tlyB, bhlp16, bhlp29.7, and bhmp39f). Results of gene expression analysis were variable, with up- and downregulations not properly correlated with particular substances or target genes. Decanoic and dodecanoic acid with their direct and indirect antimicrobial property were the most effective among MCFA, suggesting them as good candidates for subsequent in vivo trials.

show abstract

“… Brachyspira hyodysenteriae was confirmed to cause swine dysentery in Swiss pig herds in 2008 ( 1 ). Since then, isolates have been characterized by multilocus sequence typing and determination of their antimicrobial resistance mechanisms ( 2 , 3 ). To date, only four sequence types (STs) (ST6, ST66, ST196, and ST197) of B. hyodysenteriae have been identified, prompting us to sequence their complete genomes.…”

Section: Announcementmentioning

confidence: 99%

Complete Circular Genome Sequences of Brachyspira hyodysenteriae Isolates of the Four Different Sequence Types Causing Swine Dysentery in Switzerland

García-Martín

Schmitt

Zeeh

et al. 2021

Microbiol Resour Announc

Self Cite

View full text Add to dashboard Cite

The complete genomes of four Brachyspira hyodysenteriae isolates of the four different sequence types (STs) (ST6, ST66, ST196, and ST197) causing swine dysentery in Switzerland were generated by whole-genome sequencing and de novo hybrid assembly of reads obtained from second (Illumina) and third (Oxford Nanopore Technologies and Pacific Biosciences) high-throughput sequencing platforms.

show abstract

Predominance of a macrolide-lincosamide-resistant Brachyspira hyodysenteriae of sequence type 196 in Swiss pig herds

Cited by 12 publications

References 24 publications

Characterization of a NDM-1- Encoding Plasmid pHFK418-NDM From a Clinical Proteus mirabilis Isolate Harboring Two Novel Transposons, Tn6624 and Tn6625

Characterization of a NDM-1- Encoding Plasmid pHFK418-NDM From a Clinical Proteus mirabilis Isolate Harboring Two Novel Transposons, Tn6624 and Tn6625

Dual Antimicrobial Effect of Medium-Chain Fatty Acids against an Italian Multidrug Resistant Brachyspira hyodysenteriae Strain

Complete Circular Genome Sequences of Brachyspira hyodysenteriae Isolates of the Four Different Sequence Types Causing Swine Dysentery in Switzerland

Contact Info

Product

Resources

About