Predilection of a nasopharyngeal carcinoma‐derived isolate of epstein‐barr virus for infection of specific subsets of B lymphocytes

Tarr, Kathleen L.; Glaser, Ronald

doi:10.1002/jmv.1890290109

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Studies have demonstrated that strain differences exist among isolates of EBV (34,35). However, the importance of these strain differences in virus replication and in the ability of EBV to cause disease is unknown.…”

Section: Discussionmentioning

confidence: 99%

A monoclonal antibody that neutralizes Epstein-Barr virus, human cytomegalovirus, human herpesvirus 6, and bacteriophage T4 DNA polymerases.

Tsai¹,

Williams²,

Glaser³

1990

Proc. Natl. Acad. Sci. U.S.A.

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A monoclonal antibody (mAb) designated 55H3 was produced by using chemically induced Epstein-Barr virus genome-positive B95-8 cells. mAb 55H3, which reacted with an 85-to 80-kDa polypeptide, neutralized Epstein-Barr virus-encoded DNA polymerase activity in crude extracts of chemically induced M-ABA, HR-1, and B95-8 cells, as well as the partially purified Epstein-Barr virus DNA polymerase in a dose-dependent manner. The mAb also neutralized the virusencoded DNA polymerase activity from cells infected with human cytomegalovirus, human herpesvirus 6, and the purified bacteriophage T4 DNA polymerases. However, mAb 55H3 did not neutralize the DNA polymerase activities encoded for by herpes simplex virus types 1 and 2, the reverse transcriptase of avian myeloblastosis virus, or Escherichia coli DNA polymerase 1 (Klenow fragment). These results suggest that mAb 55H3 recognizes an epitope common to some herpesviruses and T4 DNA polymerases and further supports the hypothesis that these organisms are evolutionarily related.Members of the poxviruses, adenoviruses, and herpesviruses encode specific DNA polymerases (1-6). These DNA polymerases are not only required for replication of these viruses, but they can also be used as a target site for development of antiviral agents. While there have been advances in our understanding of the conformation of various DNA polymerases and their interaction with various substrates and with template DNA, little is known about the interaction of viral DNA polymerase with other proteins involved in the DNA replication complex.Epstein-Barr virus (EBV), a human herpesvirus, is the etiological agent of infectious mononucleosis and is associated with African Burkitt lymphoma and nasopharyngeal carcinoma (7-11). EBV is similar to other herpesviruses in that it encodes a DNA polymerase essential for virus replication (12, 13). The EBV-encoded DNA polymerase activity has been purified from several EBV genome-positive cells, and at least four polypeptides with molecular masses of 110, 100, 55, and 49 kDa have been associated with this activity (14,15). Although the 110-kDa polypeptide may be the DNA polymerase (14), studies have demonstrated that the 55-and 49-kDa proteins (15), as well as a 45-kDa stimulatory protein (16), are required for EBV DNA polymerase activity in vitro. Elucidation of these protein interactions is important not only for understanding how EBV replicates but also in determining the role of these polypeptides in diseases caused by EBV.While sera from patients with nasopharyngeal carcinoma contain antibodies that neutralize EBV DNA polymerase, the sera also contain antibodies against the EBV DNA polymerase stimulatory protein (16)(17)(18)(19). Similarly, although a rabbit polyclonal antibody has been developed that can inhibit EBV DNA polymerase activity (20), it is not known whether this polyclonal antibody neutralizes the DNA polymerase activity by binding to the enzyme or due to its binding to one of the associated polypeptides. Thus, further studies on the intera...

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Section: Discussionmentioning

confidence: 99%

A monoclonal antibody that neutralizes Epstein-Barr virus, human cytomegalovirus, human herpesvirus 6, and bacteriophage T4 DNA polymerases.

Tsai¹,

Williams²,

Glaser³

1990

Proc. Natl. Acad. Sci. U.S.A.

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Spontaneous development of a chromosomal translocation 5;14 in an epstein‐barr‐virus‐associated b‐cell lymphoma in a SCID mouse

Glaser

Theil

Bonneau³

et al. 1993

Intl Journal of Cancer

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C.B-17 SCID mice were inoculated with human peripheral blood leukocytes (PBLs) from normal Epstein-Barr virus (EBV)-seropositive and -seronegative donors. Confirmation of a functioning human immune response was demonstrated by the detection of human antibody after inoculation with rotavirus, tetanus toxoid, or EBV. One group of animals inoculated with PBLs from an EBV-seropositive donor developed immunoblastic lymphomas approximately 9 weeks after transplantation. Confirmation of the species and sex of origin of the tumor cells was established using a spontaneous cell line prepared from the tumor. At passage I, the tumor-cell line (AGTI) showed 15% of the metaphases with a translocation involving chromosomes 5 and 14. A lymphoblastoid cell line (AGLCL) established from the same PBLs from the same donor at the time of inoculation of the mice had a normal female karyotype. The AGLCL and a clone of AGTI cells were analyzed for rearrangement of immunoglobulin heavy chain (IgH) genes; both cell lines showed rearrangement of both IgH alleles. The results outlined in this report suggest that a spontaneous chromosomal translocation involving chromosome 14 occurred in normal PBLs in the SCID mouse.

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The Epstein-Barr virus and nasopharyngeal carcinoma

Tarr

Glaser

1989

Microbial Pathogenesis

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Predilection of a nasopharyngeal carcinoma‐derived isolate of epstein‐barr virus for infection of specific subsets of B lymphocytes

Cited by 3 publications

References 23 publications

A monoclonal antibody that neutralizes Epstein-Barr virus, human cytomegalovirus, human herpesvirus 6, and bacteriophage T4 DNA polymerases.

A monoclonal antibody that neutralizes Epstein-Barr virus, human cytomegalovirus, human herpesvirus 6, and bacteriophage T4 DNA polymerases.

Spontaneous development of a chromosomal translocation 5;14 in an epstein‐barr‐virus‐associated b‐cell lymphoma in a SCID mouse

The Epstein-Barr virus and nasopharyngeal carcinoma

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