Predictive value of skin prick tests using recombinant allergens for diagnosis of peanut allergy

Astier, Catherine; Morisset, M.; Roitel, Olivier; Codréanu, F.; Jacquenet, S.; Franck, P.; Ogier, Virginie; Petit, Nicolas; Proust, B.; Moneret-Vautrin, D. A.; Burks, A. Wesley; Bihain, Bernard E.; Sampson, Hugh A.; Kanny, G.

doi:10.1016/j.jaci.2006.04.053

Cited by 197 publications

(185 citation statements)

References 39 publications

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“…Compound 48/80 was obtained from Sigma (St. Louis, Mo., USA). Major recombinant peanut allergens rAra h 1, rAra h 2 and rAra h 3 were produced and purified in our laboratory [19]. …”

Section: Methodsmentioning

confidence: 99%

A Single Oral Sensitization to Peanut without Adjuvant Leads to Anaphylaxis in Mice

Proust¹,

Astier²,

Jacquenet³

et al. 2008

Int Arch Allergy Immunol

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Background: A model of peanut food allergy has been developed in mice using a simple sensitization protocol leading to a quantitatively measurable allergic response. Methods: C3H/HeJ mice received a single intragastric administration of whole peanut (80 mg) without adjuvant. Two weeks later, intraperitoneal challenge with peanut extract led to a severe anaphylaxis. Results: Anaphylactic reaction was evidenced by vascular leakage, severe clinical symptoms, a drop in body temperature, a decrease in breathing rate and also by increased concentrations of serum mouse mast cell protease-1. Sensitization to peanut was demonstrated by positive skin tests (ear swelling test and intradermal skin testing) and increased peanut-specific IgE levels. Conclusions: Thus, we obtained a model of severe peanut hypersensitivity within 2 weeks following single oral exposure without adjuvant. This model may be useful for further basic and applied studies on peanut allergy.

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“…Compound 48/80 was obtained from Sigma (St. Louis, Mo., USA). Major recombinant peanut allergens rAra h 1, rAra h 2 and rAra h 3 were produced and purified in our laboratory [19]. …”

Section: Methodsmentioning

confidence: 99%

A Single Oral Sensitization to Peanut without Adjuvant Leads to Anaphylaxis in Mice

Proust¹,

Astier²,

Jacquenet³

et al. 2008

Int Arch Allergy Immunol

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“…Recombinant allergens are useful for the diagnosis of food allergy such as apple (525,1216), celery (553), peanut (1217) or cherry (1218). Skin tests with recombinant food allergens can be an alternative to prick-prick tests with foods (1219).…”

Section: Skin Testsmentioning

confidence: 99%

“…However, the diagnosis of food allergy is compounded because currently-available allergen vaccines and test reagents are not standardized and their stability is poorly determined (195,1381,1382). Recombinant allergens improve the diagnosis of food allergy (1217). The presence of food-specific IgE in serum or a positive skin test to a foodstuff does not always correlate with food allergic symptoms because many patients outgrow their allergy with age (1383,1384) and not all patients with food-specific IgE have a clinical sensitivity (1385).…”

Section: Diagnosis Of Inhalant Allergymentioning

confidence: 99%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

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1,761

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“…However, only a few comparative SPT studies with purified food allergens have been conducted, [28], [72], [73] and [74] which is mainly due to the high demands of approval in clinical trials as requested by the institutional review boards and regulatory authorities to ensure patient safety (test allergens have to be licensed as biotechnologic products). These few studies verified a superior diagnostic potential of recombinant allergens.…”

Section: Skin Tests With Purified Allergenic Moleculesmentioning

confidence: 99%

“…Hence, skin tests with recombinant molecules need to include panels of recombinant allergens covering all immunodominant structures present in a given food. [72] and [74] …”

Section: Skin Tests With Purified Allergenic Moleculesmentioning

confidence: 99%

Potential, pitfalls, and prospects of food allergy diagnostics with recombinant allergens or synthetic sequential epitopes

Steckelbroeck¹,

Ballmer‐Weber²,

Vieths³

2008

Journal of Allergy and Clinical Immunology

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This article aims to critically review developments in food allergy diagnostics with regard to the verification of specific IgE antibodies and the identification of the responsible allergens. Results of IgE-binding tests with food extracts are hampered by cross-reactive proteins, low-quality test agents, or both. Specificity can be increased by defining adequate cutoff values, whereas sensitivity can be improved by using high-quality test agents. IgE-binding tests with purified allergens enabled reliable quantification of allergen-specific IgE titers, with higher levels found in individuals with food allergy compared with individuals without food allergy. However, the overlap in individual test reactivity between allergic and nonallergic subjects complicates interpretation. Recombinant allergens and synthetic sequential epitopes enabled detection of sensitization profiles, with IgE specific to several allergens and substructures now being suggested as markers of severity, persistence, or both. However, high-power quantitative studies with larger numbers of patients are required to confirm these markers. This article aims to critically review developments in food allergy diagnostics with regard to the verification of specific IgE antibodies and the identification of the responsible allergens. Results of IgE-binding tests with food extracts are hampered by cross-reactive proteins, low-quality test agents, or both. Specificity can be increased by defining adequate cutoff values, whereas sensitivity can be improved by using high-quality test agents. IgE-binding tests with purified allergens enabled reliable quantification of allergen-specific IgE titers, with higher levels found in individuals with food allergy compared with individuals without food allergy. However, the overlap in individual test reactivity between allergic and nonallergic subjects complicates interpretation. Recombinant allergens and synthetic sequential epitopes enabled detection of sensitization profiles, with IgE specific to several allergens and substructures now being suggested as markers of severity, persistence, or both.However, high-power quantitative studies with larger numbers of patients are required to confirm these markers. IgE-mediated and non-IgE-mediated syndromes, where IgE-mediated manifestations are responsible for the majority of food-induced, immediate-type, immune-mediated hypersensitivity reactions. 3 The development of an IgE-mediated response to food requires a series of molecular and cellular interactions, which involve antigenpresenting cells, T lymphocytes, and B lymphocytes. [4] and [5] Depending on the route of sensitization, food allergy is the result of either genuine reactivity to comestibles through the gastrointestinal tract (class I food allergens) or secondary sensitization to cross-reactive food allergens as a consequence of the initial reactivity to homologous pollen-related allergens (class II food allergens). [6] and [7] The majority of class I food allergens are heat stable and resistan...

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Predictive value of skin prick tests using recombinant allergens for diagnosis of peanut allergy

Cited by 197 publications

References 39 publications

A Single Oral Sensitization to Peanut without Adjuvant Leads to Anaphylaxis in Mice

A Single Oral Sensitization to Peanut without Adjuvant Leads to Anaphylaxis in Mice

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Potential, pitfalls, and prospects of food allergy diagnostics with recombinant allergens or synthetic sequential epitopes

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