A Single Oral Sensitization to Peanut without Adjuvant Leads to Anaphylaxis in Mice

Abstract

Background: A model of peanut food allergy has been developed in mice using a simple sensitization protocol leading to a quantitatively measurable allergic response. Methods: C3H/HeJ mice received a single intragastric administration of whole peanut (80 mg) without adjuvant. Two weeks later, intraperitoneal challenge with peanut extract led to a severe anaphylaxis. Results: Anaphylactic reaction was evidenced by vascular leakage, severe clinical symptoms, a drop in body temperature, a decrease in breathing rate and also by increased concentrations of serum mouse mast cell protease-1. Sensitization to peanut was demonstrated by positive skin tests (ear swelling test and intradermal skin testing) and increased peanut-specific IgE levels. Conclusions: Thus, we obtained a model of severe peanut hypersensitivity within 2 weeks following single oral exposure without adjuvant. This model may be useful for further basic and applied studies on peanut allergy.

IgG1 + ovalbumin-specific B-cell transnuclear mice show class switch recombination in rare allelically included B cells

“…Given that the OBI mice already have a preexpanded pool of antigen-specific B cells and high serum titers of specific antibodies, we were curious as to whether OBI mice would phenocopy ovalbumin-sensitized mice with respect to food allergies. Administration of ovalbumin via the drinking water or by oral gavage of OBI mice did not induce an acute drop in body temperature characteristic of anaphylaxis (30). These results are consistent with the lack of detectable IgE.…”

Specific epicutaneous immunotherapy prevents sensitization to new allergens in a murine model

“…21,22,26,27 In brief, milk-sensitized mice were orally sensitized to milk proteins for 6 consecutive weeks by means of weekly gavage with 5 mg of cow's milk protein (CMP) from commercial whole cow's milk 26 Peanut-sensitized mice were orally sensitized to peanut for 2 consecutive weeks by means of weekly gavage with 80 mg of peanut protein extract (PPE) supplemented with 10 mg of CT, a protocol adapted from Adel-Patient et al 26 and Proust et al 27 Sensitization to aeroallergens (HDM or Phleum pratense pollen) was performed by using 2 subcutaneous injections of 100 mg of protein extract added with 1 mg of aluminum hydroxide, followed by an intranasal administration of 10 mg of protein at day 15. 21 Each treatment group was composed of 10 mice.…”

Effective induction of oral anaphylaxis to ovalbumin in mice sensitized by feeding of the antigen with aid of oil emulsion and salicylate

“…Oral sensitization models without an adjuvant have also been proposed. One is the sensitization model using oral immunization with protein alone but at unusually high dose (Proust et al, 2008). The other is the transdermal immunization of protein antigen alone (Hsieh et al, 2003;Birmingham et al, 2007).…”

“…Moreover, attempts have been made to create adjuvant-free sensitization mouse models. In a mouse model, antigen given orally, in excess and in isolation, was sufficient to induce antigen-specific IgE (Proust et al, 2008). Subsequently, anaphylaxis to the antigen was shown with intraperitoneal challenge, although oral challenge was not performed.…”