Predictive Value of in Vitro Model Systems in Toxicology

Dávila, Julio C.; Rodriguez, Russell J.; Melchert, Russell B.; Acosta, Daniel

doi:10.1146/annurev.pharmtox.38.1.63

Cited by 120 publications

(87 citation statements)

References 92 publications

(161 reference statements)

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“…In many cases, based on their predictive results, in vivo testing does not occur. Another important advantage of in vitro system is the possibility to define strict experimental conditions with the elimination of external factor influence (Davila et al 1998). In addition, in vitro system provides more information about the mechanisms of action of tested compounds, which is difficult to obtain from animal model due to its complexity (Barber et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1

et al. 2010

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In the present study, human neonatal fibroblasts were isolated from a two-month-old human male. The purpose of the present investigation was the analysis of the morphology (light and transmission electron microscopy), karyotype and growth characteristics of the human neonatal fibroblast cell culture B-HNF-1. Moreover, STR typing and mitochondrial DNA amplification and sequencing was also performed. Analysis of chromosomes count showed that B-HNF-1 cell culture is diploid and has normal male karyotype 46, XY, which was stable during cultivation. The transmission electron microscopy demonstrated the ultra-structure of the B-HNF-1 cells; they have typical morphological features of proteosynthesis-active cells. Large number of fibroblasts bearing different shapes and surface characteristics adhered to the substrate with microvilli and filopodia. Our in vitro expanded fibroblasts have a large and irregular nucleus with prevalence of euchromatin. One to three nucleoli are present in each nucleus. The cytoplasm contains a richly developed rough endoplasmic reticulum and prominent Golgi apparatus cisterns. The result of the fragment analysis is a DNA profile defined as multiplex of STR markers and sex determining system. Sequencing analysis of hypervariable segments of control region of mitochondrial DNA showed haplotype that belongs to haplogroup W. In this study, we complexly characterized a new cell culture of human neonatal fibroblasts B-HNF-1 from different points of view. This culture should be used for further biomedical experiments.

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Section: Introductionmentioning

confidence: 99%

Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1

et al. 2010

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“…The alveolar epithelium cells are subjected to variety of chemical and mechanical stimuli because of infections, inhaled particles and complications from various diseases. 37 Researchers and scientist found out the in-vitro model by the use of Trans wells to mimic air-liquid interface of lung barrier tissues. Now-adays 2-layered PDMS micro-fluidic devices is used.…”

Section: Lungs-on-chipmentioning

confidence: 99%

Non-Animal Models for Research and Toxicity Testing of Drugs

Ajmera¹,

Shendge²

2017

IJPER

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All drug discovery methods, right from 'trial-and-error' approach to modern rational synthesis of drugs, make use of animals to check for efficacy and toxicity of compounds. This results in millions of animals being sacrificed every year. Following a global debate on "cruelty of animals", various acts were made and enforced in order to protect animal rights. As a work, around to these animal protection acts, drug discovery scientists started finding alternative approaches to animal studies for research. This review attempts to compile some of the more important non-animal models which are being used for testing safety and efficacy of drugs in preclinical stages.

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“…Draize testi tekrarlanabilir olmayışı, test süresinin uzunluğu, son noktanın değerlendirilmesindeki sübjektiflik, test maddesinin uygulanmasındaki zorluklar, yüksek doz ve türler arasındaki farklılıklar nedeniyle uzun yıllardır eleştirilmektedir (16,17). Bu testte sıvı ve katı maddelerin göz üzerine uygulanmasında iyi tanımlanamamış farklı temas süreleri vardır (18).…”

Section: Drai̇ze Göz İrri̇tasyon Testi̇unclassified

“…Patolojik değişiklikler iris, kornea ve konjonktiva ile karakterize edilir (19). LVET özellikle kozmetik, farmasötik ve ev ürünlerinde bulunan surfaktan bazlı karışımlar için uygulanmaktadır (16). Şiddetli irritan maddelerin analizlerinde LVET ve Draize testlerinden elde edilen sonuçlar arasında farklılıklar olduğu gözlenmiştir.…”

Section: Drai̇ze Göz İrri̇tasyon Testi̇unclassified

“…Epitel modeller ile ilgili sınırlandırmalardan biri hücresel izolasyonu engelleyen korneal dokuda başlangıçtaki enzimatik ve mekanistik disasosiasyonun da hücrede farklı birçok cevap oluşturabilecek travmatik uyarıların oluşmasına neden olabilmesidir (16). Bu durum, hücresel yapı bütünlüğünün bozulmasına, inflamatuvar mediatörlerin salgılanmasına ya da hücre farklılaşmasına neden olabilmektedir.…”

Section: In Vitro Alternatif Yöntemlerin Sınırlamalarıunclassified

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Kozmetik Ürünlerin Göz İrritasyon Potansiyellerinin Değerlendirilmesinde Geleneksel ve Alternatif Yöntemler

Köse¹,

Erkekoğlu²,

Sabuncuoğlu³

et al. 2017

Marmara Pharmaceutical Journal

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For several years, In vivo ocular toxicity tests were used to evaluate the hazard potential of drugs and chemicals that are applied to eye. In ethical terms, eye irritation tests can cause discomfort and pain in laboratory animals. Since 3R principle has been published, several alternative methods have been developed, validated and accepted by regulatory authorities to evaluate the potential toxicity of chemicals and cosmetics. On March 11 st 2013, the commercial sales of any types of cosmetics and personal care products tested on animals, were banned in the European Union. Since then, alternative in vitro toxicity tests are used for the safety evaluation of the cosmetics. For the evaluation of the ocular toxicity of chemicals and cosmetics, the In vitro restructured human ocular tissue models are used as an alternative methods. Some of these models are preferred more because of their morphological and physiological similarity to the human eye. In this review, both in vitro and in vivo ocular toxicity tests will be mentioned; newly developed and validated test methods will be evaluated with a comprehensive perspective.

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Predictive Value of in Vitro Model Systems in Toxicology

Cited by 120 publications

References 92 publications

Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1

Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1

Non-Animal Models for Research and Toxicity Testing of Drugs

Kozmetik Ürünlerin Göz İrritasyon Potansiyellerinin Değerlendirilmesinde Geleneksel ve Alternatif Yöntemler

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