Predictive modeling and cryo-EM: A synergistic approach to modeling macromolecular structure

Corum, Michael R.; Venkannagari, Harikanth; Hryc, Corey F.; Baker, Matthew L.

doi:10.1016/j.bpj.2024.01.021

Biophysical Journal

2024

DOI: 10.1016/j.bpj.2024.01.021

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Predictive modeling and cryo-EM: A synergistic approach to modeling macromolecular structure

Michael R. Corum,

Harikanth Venkannagari,

Corey F. Hryc

et al.

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2024

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Unveiling the stochastic nature of human heteropolymer ferritin self‐assembly mechanism

Bou‐Abdallah,

Fish,

Terashi

et al. 2024

Protein Science

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Despite ferritin's critical role in regulating cellular and systemic iron levels, our understanding of the structure and assembly mechanism of isoferritins, discovered over eight decades ago, remains limited. Unveiling how the composition and molecular architecture of hetero‐oligomeric ferritins confer distinct functionality to isoferritins is essential to understanding how the structural intricacies of H and L subunits influence their interactions with cellular machinery. In this study, ferritin heteropolymers with specific H to L subunit ratios were synthesized using a uniquely engineered plasmid design, followed by high‐resolution cryo‐electron microscopy analysis and deep learning‐based amino acid modeling. Our structural examination revealed unique architectural features during the self‐assembly mechanism of heteropolymer ferritins and demonstrated a significant preference for H‐L heterodimer formation over H‐H or L‐L homodimers. Unexpectedly, while dimers seem essential building blocks in the protein self‐assembly process, the overall mechanism of ferritin self‐assembly is observed to proceed randomly through diverse pathways. The physiological significance of these findings is discussed including how ferritin microheterogeneity could represent a tissue‐specific adaptation process that imparts distinctive tissue‐specific functions to isoferritins.

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Unveiling the stochastic nature of human heteropolymer ferritin self‐assembly mechanism

Bou‐Abdallah,

Fish,

Terashi

et al. 2024

Protein Science

View full text Add to dashboard Cite

show abstract

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Predictive modeling and cryo-EM: A synergistic approach to modeling macromolecular structure

Cited by 1 publication

References 102 publications

Unveiling the stochastic nature of human heteropolymer ferritin self‐assembly mechanism

Unveiling the stochastic nature of human heteropolymer ferritin self‐assembly mechanism

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