Predictive factors that affect the mobilization of CD34+cells in healthy donors treated with recombinant granulocyte colony-stimulating factor (G-CSF)

Abstract

No specific characteristics have been identified as predictors of peripheral blood stem cells (PBSC) mobilization in healthy donors. In this study, clinical characteristics and laboratory data for 122 healthy donors who underwent apheresis on day 5 of treatment with recombinant granulocyte colony-stimulating factor (G-CSF) were retrospectively analyzed for correlations with CD34(+) cell mobilization. The variables that were analyzed included age, sex, body weight, basal complete blood count, and maximum white blood count (WBC) before apheresis, G-CSF type, and dosage. Median age and body weight were 42.5 years (range 16-65) and 72.5 kg (range 47-121), respectively. By univariate analysis, male sex (P = 0.007), body weight (< or = 70 vs. >70 kg, P = 0.04), and donor's age (< or = 50 vs. > 50 years; P = 0.015) were correlated with the number of CD34(+) cells mobilized. By multivariate analysis, donor's age and male sex were the only two variables that significantly predicted a high CD34(+) cell level. In conclusion, our data suggest that male sex and younger age are the only factors that significantly affect CD34(+) mobilization in healthy donors.

Efficacy and safety of peripheral blood stem cell mobilization and collection: a single‐center experience in 190 allogeneic donors

“…Also in our study, as previously published in other case series, 9,19‐21 80% of donors reached the target dose for transplantation (CD34+≥4 × 10 6 /kg recipient in HLA‐full‐matched transplants). We registered only 4% (7/190) failures of PBSC collection (CD34+ <2.0 × 10 6 /kg recipient), requiring marrow harvest or a second donor mobilization, but we could not identify predictive variables others than the female sex and the premobilization CD34+, already mentioned.…”

Prediction of hematopoietic stem cell yield after mobilization with granulocyte–colony‐stimulating factor in healthy unrelated donors

“…Vasu and colleagues reported a reduced post–G‐CSF CD34+ cell count in females in their analysis of 639 allogeneic donors. Others found the same correlation …”

A review of the haematopoietic stem cell donation experience: is there room for improvement?

“…Restricting the recruitment to 'lighter' donors would carry an inherent conflict as several studies have shown that stem cell yields are positively correlated with donor weight. 83,84 The management of donors' expectations can similarly be important, especially in donors at increased risk of ARs. High-risk groups ought to be made aware of this at the time of their medical assessment.…”