Prediction of hematopoietic stem cell yield after mobilization with granulocyte–colony‐stimulating factor in healthy unrelated donors

Teipel, Raphael; Schetelig, Johannes; Kramer, Michael; Schmidt, Helmuth; Schmidt, Alexander H.; Thiede, Christian; Oelschlägel, Uta; Kroschinsky, Frank; Bornhäuser, Martin; Ehninger, Gerhard; Hölig, Kristina

doi:10.1111/trf.13239

Cited by 35 publications

(44 citation statements)

References 32 publications

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“…In the European set, only BMI correlated with circulating CD34+ cell counts. The mechanism behind association between higher BMI and better mobilization potential has been attributed to the effect of adipose tissue-containing HSCs, or a simple dose effect of G-CSF [8]. …”

Section: Discussionmentioning

confidence: 99%

“…There is wide inter-individual variability in response to mobilization, and the outcome is hardly predictable despite several known demographic or clinical risk factors such as the following: age, sex, body mass index (BMI), ethnicity, diagnosis, and extent and duration of prior chemotherapy [4–8]. Inter-individual variation of HSC mobilization yield can be explained by a multifactorial model consisting of environmental and multiple genetic factors.…”

Section: Introductionmentioning

confidence: 99%

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A novel association between relaxin receptor polymorphism and hematopoietic stem cell yield after mobilization

et al. 2017

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Mobilization of hematopoietic stem cells (HSCs) from the bone marrow to the peripheral blood is a complex mechanism that involves adhesive and chemotactic interactions of HSCs as well as their bone marrow microenvironment. In addition to a number of non-genetic factors, genetic susceptibilities also contribute to the mobilization outcome. Identification of genetic factors associated with HSC yield is important to better understand the mechanism behind HSC mobilization. In the present study, we enrolled 148 Korean participants (56 healthy donors and 92 patients) undergoing HSC mobilization for allogeneic or autologous HSC transplantation. Among a total of 53 polymorphisms in 33 candidate genes, one polymorphism (rs11264422) in relaxin/insulin-like family peptide receptor 4 (RXFP4) gene was significantly associated with a higher HSC yield after mobilization in Koreans. However, in a set of 101 Europeans, no association was found between circulating CD34+ cell counts and rs11264422 genotype. Therefore, we suggest that the ethnic differences in subjects’ genetic background may be related to HSC mobilization. In conclusion, the relaxin—relaxin receptor axis may play an important role in HSC mobilization. We believe that the results of the current study could provide new insights for therapies that use relaxin and HSC populations, as well as a better understanding of HSC regulation and mobilization at the molecular level.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel association between relaxin receptor polymorphism and hematopoietic stem cell yield after mobilization

et al. 2017

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“…Other factors such as iron status have been recently evaluated . However, factors identified in these cohorts have not explained more than a 20%‐30% of the variability observed …”

Section: Introductionmentioning

confidence: 99%

“…Previous studies had indicated that there is an inter‐individual variation in progenitor cell mobilization among healthy subjects. It has also been reported that donor's sex, age, weight, white blood cell (WBC) and platelet count, among others, correlate with the CD34+ cell yield . Other factors such as iron status have been recently evaluated .…”

Section: Introductionmentioning

confidence: 99%

Factors predicting peripheral blood progenitor cell mobilization in healthy donors in the era of related alternative donors: Experience from a single center

Bailén

Pérez‐Corral

Pascual

et al. 2019

J of Clinical Apheresis

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Background Poor mobilization results are unexpected after G‐CSF‐induced peripheral blood stem cell collection in healthy donors. However, 2%‐5% of the donors are poor mobilizers. Factors predicting CD34+‐cell yield after mobilization in related alternative donors are still poorly known. Patients and methods Baseline characteristics and efficacy results of G‐CSF induced mobilization of 159 adult healthy donors in our institution from 2008 to 2016 were retrospectively analyzed. All donors received 10 μg/kg of G‐CSF once a day subcutaneously for 4 days. Leukapheresis started on the 5th day of G‐CSF treatment. Donors were classified as poor mobilizers if they had less than 20 000 CD34 + cell/mL peripheral blood count in the 5th day of G‐CSF treatment or if they needed three or more leukapheresis for graft collection. Results Age, weight, and platelet count before and after mobilization were significantly different between poor and good mobilizers. Poor mobilizers (n = 16) were older (50.6 vs 41.7 years, P = 0.002), weight lower (64 vs 75 kg, P = 0.00) and showed a lower platelet count before (199.5 vs 219.0 × 109/L, P = 0.03) and after (192.5 vs 206 × 109/L, P = 0.019) mobilization. In the multivariate analysis only the 30% of the variability of mobilization was explained by the model (sensitivity 80%, specificity 70%). Conclusion In this cohort of healthy donors in a single institution, older age, less weight, and lower platelet count was associated with poorer mobilization. With clinical and analytic factors it is not possible to predict more than 30% of the variability. Further studies are needed to investigate new variables.

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“…Therefore, it is desirable to have a reliable prediction method in order to avoid unnecessarily prolonged collection for the safety of donors. Some prediction methods have been proposed and have proven useful [14,15]. In the present study, we introduced a simple method to predict PBSC yield based on pre-CD34 count.…”

Section: Introductionmentioning

confidence: 99%

A Method to Predict the Yield of Peripheral Blood Stem Cells Collected by Large Volume Leukapheresis

J¹,

Burns²,

Kennedy³

et al. 2017

J Blood Lymph

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Background: Collection of peripheral blood stem cells (PBSC) by leukapheresis has become the dominant method in allogeneic hematopoietic stem cell donation and also overpassed bone marrow donation in autologous transplantations. The circulating CD34 positive cells immediately prior to PBSC collection strongly correlate with the PBSC yield and have used to monitor donor's response to bone marrow mobilization. Methods:In this prospective study, a prediction method for PBSC collection that has been used in our facility was provided. Prediction accuracy is evaluated by comparison of predicted CD34 values to real PBSC yield. Results:The prediction method was used to determine first if a standard procedure with 12 L of blood processing could achieve the collection goal. Thirty seven donors were considered to have adequate PBSCs. Thirty four of them had a CD34 count above the target and only one donor had a CD34 count significantly below the target. For the donors whose PBSC were considered inadequate, an extended collection was performed based on the predicted blood volume that needed to be processed. The number of overestimated predictions was essentially the same as the number of underestimated predictions. Conclusion:The proposed prediction method was successful in identifying donors who needed extended collection without causing unnecessary prolongation of the leukapheresis procedure.

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Prediction of hematopoietic stem cell yield after mobilization with granulocyte–colony‐stimulating factor in healthy unrelated donors

Abstract: The model allows the identification of female donors who eventually have a significant risk of harvest failure if requested to donate for recipients with a high body weight.

Cited by 35 publications

References 32 publications

A novel association between relaxin receptor polymorphism and hematopoietic stem cell yield after mobilization

A novel association between relaxin receptor polymorphism and hematopoietic stem cell yield after mobilization

Factors predicting peripheral blood progenitor cell mobilization in healthy donors in the era of related alternative donors: Experience from a single center

A Method to Predict the Yield of Peripheral Blood Stem Cells Collected by Large Volume Leukapheresis

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