Prediction of severe disseminated adenovirus infection by serum PCR

Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection

“…Besides the 15 viral isolates, other eight nasopharyngeal samples negative for viral isolation in cell culture were positive for AdV by PCR detection. This finding agrees with previous studies which have shown that even though viral culture has been considered as the "gold standard" for AdV diagnosis, the PCR-based assays are more sensitive for this purpose (Allard et al 1990, Pring-Åkerblom & Adrian 1994, Alistair et al 1996, Kajiwara et al 1999, Xu et al 2000, Avellón et al 2001, Echavarria et al 2001, Heim et al 2003.…”

“…Immunofluorescence and other immunodiagnostic methods using direct antigen detection are faster than culture, but they lack sensitivity (Minnich & Ray 1980, Mahafzah & Landry 1989, Portillo & Cruz 2000. Recently polymerase chain reaction (PCR)-based assays have proven to be useful for AdV detection, showing higher specificity and sensitivity in comparison to the classic methods (Allard et al 1990, Pring-Åkerblom & Adrian 1994, Alistair et al 1996, Kajiwara et al 1999, Xu et al 2000, Avellón et al 2001, Echavarria et al 2001, Heim et al 2003.…”

Evaluation of a surveillance strategy for early detection of adenovirus by PCR of peripheral blood in hematopoietic SCT recipients: incidence and outcome

“…Detection of AdV DNA in peripheral blood and especially rapid increase in the viral load is associated with progression to disseminated AdV disease. 9,11,26,31 It would therefore be logical to identify what group of patients to screen and in what period after HSCT. As adults seem to get their AdV infection later in the post transplantation period, and the risk for AdV disease seems to be low, 25 a sampling schedule parallel to CMV screening may not be adequate.…”

“…[1][2][3][4][5][6][7][8] The risk of disseminated disease has been shown to correlate positively with viral load as measured by quantitative PCR methods. [9][10][11] Cidofovir has emerged as the primary therapy for AdV infection. The drug was recently evaluated in a highrisk pediatric population and was found to be safe and mostly well tolerated.…”

Adenovirus Fulminant Hepatic Failure: Disseminated Adenovirus Disease after Unrelated Allogeneic Stem Cell Transplantation for Acute Lymphoblastic Leukemia

“…Therapeutic options for ADV disseminated disease are still very limited (1,7,10,36,38,39,41,43,(45)(46)(47). Intravenous or aerosolized ribavirin (1,7,19,38,45,47), vidarabine (7), and cidofovir (7,36,(45)(46)(47) are included in antiviral agents, and the intravenous administration of immunoglobulin (12), the discontinuation of antibiotics (36), donor lymphocyte infusion (7) (38).…”