Prediction of embryo developmental potential and pregnancy based on early stage morphological characteristics

Sjöblom, Peter; Menezes, Judith; Cummins, Lisa; Mathiyalagan, Bagyalakshmi; Costello, Michael

doi:10.1016/j.fertnstert.2006.03.040

Cited by 58 publications

(35 citation statements)

References 55 publications

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“…This is consistent with previous reports from this study group and others (Hu et al, 1998;Lee et al, 2006;Loi et al, 2008;Qian et al, 2008;Sjöblom et al, 2006). Both the total embryo cumulative score and transferred embryo cumulative score were considerably reduced in the nonpregnant group compared with patients having live births after IVF.…”

Section: Discussionsupporting

confidence: 93%

“…There is a growing body of research showing a link between high sperm DNA damage and poor outcomes, including fertilization rate, pre-and post-implantation embryo development and implantation rate (Anendano and Oehninger, 2011;Hu et al, 1998;Qian et al, 2008;Sjöblom et al, 2006). This has also been reported for the multiparous pig where the number of piglets per litter is reduced with higher sperm DNA damage (Ballachey et al, 1988).…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Sperm DNA damage has a negative association with live-birth rates after IVF

Lee

Proutski

Stevenson

et al. 2013

Reproductive BioMedicine Online

181

137

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Sperm DNA damage has a negative association with live-birth rates after IVF, Reproductive BioMedicine Online (2012), doi: http://dx.doi.org/10. 1016/j.rbmo.2012.09.019 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Sperm DNA damage negatively affects live-birth rates in IVF patientsSperm DNA damage has a negative association with live-birth rates after IVF AbstractSperm DNA damage has a negative impact on pregnancy rates following assisted reproduction treatment. The aim of the present study was to examine the relationship between sperm DNA fragmentation and live-birth rates after IVF and intracytoplasmic sperm injection (ICSI). The alkaline Comet assay was employed to measure sperm DNA fragmentation in native semen and in spermatozoa following density-gradient centrifugation in semen samples from 203 couples undergoing IVF and 136 couples undergoing ICSI. Men were divided into groups according to sperm DNA damage. Following IVF, couples with <25% sperm DNA fragmentation had a livebirth rate of 33%; in contrast, couples with <50% sperm DNA fragmentation had a much lower live-birth rate of 13%. Following ICSI, no significant differences in sperm DNA damage were found between any groups of patients. Sperm DNA damage was also associated with low livebirth rates following IVF in both men and couples with idiopathic infertility: 39% of couples and 41% of men with idiopathic infertility have high sperm DNA damage. Sperm DNA damage assessed by the Comet assay has a close inverse relationship with live-birth rates after IVF.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Sperm DNA damage has a negative association with live-birth rates after IVF

Lee

Proutski

Stevenson

et al. 2013

Reproductive BioMedicine Online

181

137

View full text Add to dashboard Cite

show abstract

“…Sjoblom et al [17] illustrated that when they cultured embryos that were classified as unsuitable for freezing or transfer on day 3, they still obtained a number of good quality blastocysts. Hardarson et al [18] also reported that good quality blastocysts can develop from embryos classified as suboptimal.…”

Section: Discussionmentioning

confidence: 99%

The clinical outcomes of day 3 4-cell embryos after extended in vitro culture

Zhao

Lian

et al. 2014

J Assist Reprod Genet

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Objective To evaluate the development potential and clinical significance of day 3 4-cell embryos after extended in vitro culture. Methods This study was a retrospective cohort study for patients with infertility treatment between January 2011 and July 2013. Patients undergoing blastocyst culture in controlled ovarian hyperstimulation cycles using surplus embryos were analyzed in the study. A total of 764 women undergoing blastocyst culture with 1,522 surplus 4-cell embryos on day 3 were analyzed. An additional 2,391 patients with embryos undergoing blastocyst culture during the same period with embryos having more blastomeres were chosen as control. Results After extended culture, 253 embryos from 183 cycles in the study group which developed to blastocysts were frozen, and 118 embryos were warmed in subsequent frozen embryo transfer cycles. Implantation rates, clinical pregnancy rates (PRs) and ongoing PRs were 33.3 %, 38.4 % and 31.4 %, respectively, which were similar to those of the control group. The singleton birth weights of newborns using these blastocysts showed no significant difference to that seen in the control group. Conclusion Surplus 4-cell embryos on day 3 displayed lower blastulation rates. However, once a blastocyst is obtained, it has equivalent clinical outcomes. Embryos that are developmentally lagging on day 3 can be observed in extended culture to increase the cumulative chances of a successful pregnancy.

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“…3). Many attempts have been made to identify embryos that will develop to the blastocyst stage (Rijnders and Jansen, 1998;Scott et al, 2000;Lan et al, 2003;Neuber et al, 2003;Rienzi et al, 2005;Sjö blom et al, 2006;Guerif et al, 2007). Currently, the selection of embryos for transfer is based on morphological indices (Ebner et al, 2003;Borini et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

A cycle-based model to predict blastocyst transfer cancellation

et al. 2009

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background: Extended culture for blastocyst transfer is thought to result in embryos with high implantation potential, enabling the transfer of fewer embryos and the reduction of multiple pregnancies after in vitro fertilization (IVF). However, one major drawback of extended culture is the risk of transfer cancellation if no blastocyst develops by Day 5, despite the observation of adequate early embryo development. We set out to develop a model to predict blastocyst transfer cancellation. methods:The model was built from 562 consecutive first IVF cycles and tested on an independent validation cohort. Multivariable logistic regression analysis was used to test the association of patient and cycle characteristics with the presence of cultured blastocysts on Day 5 and to create a nomogram. The model's performance was quantified by discrimination and calibration and clinical utility was evaluated for various thresholds.results: Fertilization technique, number and quality ratio of the Day 3 embryos and female age were independently associated with blastocyst development. The final prediction model showed an area under the curve (AUC) of 0.75 in the training set (95% confidence interval (CI): 0.73 -0.77) and was well calibrated. The AUC for the validation data set was 0.80 (95% CI: 0.78 -0.83) and calibration was acceptable. Using a decision threshold of 0.55, the model showed a negative predictive value of 80.5% and a false positive rate of 17%.conclusion: A cycle-based model could prove clinically-relevant for reducing the incidence of cancelled Day 5 transfers.

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Prediction of embryo developmental potential and pregnancy based on early stage morphological characteristics

Cited by 58 publications

References 55 publications

Sperm DNA damage has a negative association with live-birth rates after IVF

Sperm DNA damage has a negative association with live-birth rates after IVF

The clinical outcomes of day 3 4-cell embryos after extended in vitro culture

A cycle-based model to predict blastocyst transfer cancellation

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