Sperm DNA damage has a negative association with live-birth rates after IVF

Lee, Simon; Proutski, Irina; Stevenson, Michael; Jennings, David; McManus, Joanne; Lutton, Deborah; Lewis, Sheena

doi:10.1016/j.rbmo.2012.09.019

Cited by 181 publications

(149 citation statements)

References 65 publications

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“…Recent papers show a low DNA fragmentation index associated with a higher pregnancy rate [44] and that sperm DNA damage assessed by the Comet assay has a close inverse relationship with livebirth rates after IVF [45]. Interestingly, in this study we show for the first time a significant inverse relationship between pregnancies and the DNA fragmentation detected only by the Halo test.…”

Section: Discussionsupporting

confidence: 62%

Sperm DNA Fragmentation Assays Correlate with Sperm Abnormal Morphology and the Pregnancy Outcome

Fortunato¹

2013

J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol

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Purpose: A retrospective study of 89 hypo-fertile male patients attending for in vitro fertilization was undertaken in order to evaluate possible correlations among sperm DNA damage, sperm analysis parameters and pregnancies.Methods: Sperm parameters (concentration, normal morphology and multiple anomalies) were evaluated according to the World Health Organization guidelines. DNA damages were simultaneously evaluated on each sperm sample by i) sperm chromatin decondensation test; ii) sperm DNA fragmentation evaluated by Sperm Chromatin Dispersion and Halo sperm Kit; iii) sperm DNA fragmentation evaluated by the Terminal Uridine Nick-End Labelling procedure.Results: Statistical analysis was performed by using analysis of variance and least squares regression. The sperm chromatin dispersion and fragmentation assays showed a statistically significant positive correlation (P=0.0017) in all the samples confirming the good efficacy of either of the two tests in detecting sperm DNA damage. Both the two test negatively correlated with normal sperm morphology (P=0.008), however only by using Halo sperm test we obtained a significant correlation with multiple sperm pathological morphologies (P=0.029) and an inverse correlation with pregnancies outcome (P=0.013). No correlation was detected among DNA damages, sperm concentration and chromatin decondensation. Conclusions:These data suggest a similar efficacy of sperm chromatin dispersion and Terminal Uridine NickEnd Labelling in detecting sperm DNA integrity. Due to the higher sensitivity of Halo test, its prognostic role in the diagnosis of fertilizing ability of a semen sample and possible pregnancy rate is discussed.

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Section: Discussionsupporting

confidence: 62%

Sperm DNA Fragmentation Assays Correlate with Sperm Abnormal Morphology and the Pregnancy Outcome

Fortunato¹

2013

J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol

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“…Endometriosis has been shown to be associated with higher lipid peroxidation and DNA damage [29] while bacterial vaginosis with high grade OxS levels and adverse pregnancy outcome [30]. OxSrelated DNA damage has been identified as a major contributor to poor ART outcomes, including impaired embryo development, miscarriage and birth defects in the offspring [6,12].…”

Section: -Epi (8-iso Prostaglandin F 2α ) 8-ohdg (8-hydroxy-2'-deoxmentioning

confidence: 99%

“…Despite many advances in ART, outcome is often unsuccessful: according to European data collected from 36 countries, the clinical pregnancy rates per embryo transfer were 33 % for IVF (in vitro fertilization) and 32 % for ICSI (intracytoplasmic sperm injection) procedure [3]. The frequent reasons for unsuccessful ART outcome are poor quality of germ cells or embryos, genital tract infections, uterine pathologies or endometrial receptivity or unsuitable ART method [4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Systemic oxidative stress could predict assisted reproductive technique outcome

Ahelik

Mändar

Korrovits

et al. 2015

J Assist Reprod Genet

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Purpose Previous studies have indicated that OxS (oxidative stress) may appear as a possible reason for poor ART outcome. Our aim was to study OxS levels in both partners of couples seeking Assisted reproduction Technology (ART). Methods Altogether 79 couples were recruited. Oxidative DNA damage (8-OHdG) and lipid peroxidation (8-EPI) were measured, and clinical background and ART outcomes were recorded. Results Both OxS markers accurately reflected clincal conditions with prominent negative effects attributable to genital tract infections, endometriosis, uterine myoma and smoking. Furthermore, the level of OxS was also affected by partner's state of health. The highest 8-EPI levels were detected in both partners when biochemically detectable pregnancies did not develop into clinically detectable pregnancies (in women, 97, 8±16,7 vs 72.9±22,9, p=0.007; in men, 89.6±20,4 vs 72,1± 22,6, p=0.049). Conclusions To conclude, high grade systemix OxS in both partners may negatively affect the maintenance and outcome of pregnancy. Applying the detection of OxS in ART patients may select patients with higher success rate and/or those who require antioxidant therapy. This would lead to improvement of ART outcome as well as natural fertility.

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“…Another reason for reduced expression of PLCζ could be related to oxidative stress. DNA and RNA are common targets of reactive oxygen species [37] which might have a critical effect on embryo quality and pregnancy rates in IVF/ICSI treatments [38][39][40] and increased upon heat stress or in varicocele condition [30,41].…”

Section: Discussionmentioning

confidence: 99%

An association between sperm PLCζ levels and varicocele?

Janghorban-Laricheh

Ghazavi-Khorasgani

Tavalaee

et al. 2016

J Assist Reprod Genet

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Purpose We aimed to compare the expression of phospholipase C ζ (PLCζ), as one of the main sperm factors involved in oocyte activation, at both RNA and protein levels in fertile men and those with varicocele. Methods This study included 35 individuals with male factor infertility presenting primary infertility with grade II and III unilateral varicocele and 20 fertile men without varicocele. Semen parameters were assessed according to WHO 2010. Sperm DNA fragmentation, relative expression of PLCζ at messenger RNA, and protein levels were evaluated by sperm chromatin structure assay (SCSA), real-time PCR, and Western blot analysis, respectively. Results The results of this study reveal that the mean relative expression of PLCζ was significantly lower in individuals with varicocele compared to fertile men at both transcription and translation levels. In addition, the percentage of DNA fragmentation was significantly higher in infertile men with varicocele compared to fertile men. Conclusions The findings of the present study illustrate that one of the etiologies of reduced fertility associated with varicocele is the low expression of PLCζ. This effect could subsequently reduce the sperm ability to induce oocyte activation. Therefore, these results hold promise to modify our understanding of reproductive physiology of varicocele state.

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Sperm DNA damage has a negative association with live-birth rates after IVF

Cited by 181 publications

References 65 publications

Sperm DNA Fragmentation Assays Correlate with Sperm Abnormal Morphology and the Pregnancy Outcome

Sperm DNA Fragmentation Assays Correlate with Sperm Abnormal Morphology and the Pregnancy Outcome

Systemic oxidative stress could predict assisted reproductive technique outcome

An association between sperm PLCζ levels and varicocele?

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