Predentin thickness analysis in developing and developed permanent teeth

Abstract

Background:Predentin, the unmineralized organic matrix is important in maintaining the integrity of dentin. It is usually thick where active dentinogenesis occurs. A wide variation in its thickness is reported. Hence, we determined the variation in predentin thickness at various sites of different age groups.Materials and Methods:60 freshly extracted teeth (maxillary and mandibular first premolars) were divided into three groups with 20 teeth in each as, Group 1 - teeth with incomplete root formation (age <16 years), Group 2 - teeth with complete root formation (aged between 16 and 30 years), Group 3 - teeth of patients aged above 30 years. The teeth were fixed, decalcified and sections of 6 μ thickness were obtained, and stained with hematoxylin and eosin. The distance between the odontoblastic cell layers of the pulp to the border line of the dentin was considered for the measurement of the predentin thickness. A total of nine sites were considered for each specimen.Results:The present study revealed varied mean predentin thickness at all nine sites in all three age groups. Maximum and minimum thickness was observed at the apex and pulp floor respectively in all three groups. There was a statistical significant difference in predentin thickness between groups 1 and 3 and 2 and 3.Conclusion:The predentin thickness in the first group gradually increased toward the growing end near the apex, while it was relatively constant in the second group and increased overall thickness at all the sites in the third group. A notable finding was a linear increase with age in width of the predentin and the thickness vary as a function of odontoblastic activity during different stages of tooth development.

Pulpal and periapical tissue response after direct pulp capping with endosequence root repair material and low-level laser application

“…Therefore, the null hypothesis was rejected. The predentin was most observed in the labial surface due to continuous teeth growth and its thickness varied at the pulpal level which was observed before by Basandi et al [ 40 ]. It was proven that the diode low-level laser produced more aggregated organized collagen fibrils on the pulp–dentine interface [ 19 ] and promoted the dentinogenesis process in pulp [ 41 ].…”

“…Regarding predentin thickness analysis, the captured images of predentin at 10× magnification for each group were used for measurements. Predentin thickness was measured in pixels by using ImageJ v1.53e software [ 31 , 34 ]. All values were calculated from three sites of predentin for each tooth in all groups and we calculated the means and standard deviation.…”

The emerging role of extracellular Ca2+ in osteo/odontogenic differentiation and the involvement of intracellular Ca2+ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

“…Finally, the high concentration of Ca 2+ in the distal secretory pole of the odontoblast appears to be transported to the ECM, where mineralization commences (Boskey, 1991). Here, the odontoblasts produce and modify a predentin layer of various thickness between the odontoblasts and the mineralized dentin, along with sustaining the active transport and accumulation of extracellular Ca 2+ and other ions into the mineralizing dentin, with monitoring of the entire process by odontoblasts (Basandi et al, 2015). Microelectrode measurements of Ca 2+ in predentin demonstrated a Ca 2+ activity that was three times higher than that in the dental pulp interstitial fluid.…”

Studying Effects of Calcium Oxide Nanoparticles on Dentinogenesis in Male Wistar Rats

“…Dentin is the second hardest tissue in the natural tooth after enamel and forms the overall bulk of the tooth and is not normally exposed to the oral environment [ 5 ]. The pattern and chemical composition of the dentin and bone matrix are somewhat similar.…”

“…Newly formed dentin called predentin forms at the expense of the dental pulp through a reparative, lifelong process called dentinogenesis [ 6 ]. With active dentinogenesis, the dentin layers calcify and mature throughout one's lifetime and maintain the teeth integrity [ 5 ]. Primary dentin is formed by odontoblasts which are estimated to form matrix molecules of approximately 10 µ m-thick layer and then deposited to about 4 µ m. In the root area, dentin was recognised by 15–30 µ m in thickness [ 2 ].…”