Preconcentration of trace amounts of methadone in human urine, plasma, saliva and sweat samples using dispersive liquid–liquid microextraction followed by high performance liquid chromatography

Ranjbari, Elias; Azizi, Ali; Hadjmohammadi, Mohammad Reza

doi:10.1016/j.talanta.2012.03.004

Cited by 67 publications

(32 citation statements)

References 34 publications

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“…The recovery and extraction time of the present method is better than other methods (Table 4). LOD in the proposed method is similar to the previous studies (Dams et al 2002;Leong and Huang 2008;Shamsipur and Fattahi 2011;Ranjbari et al 2012;Kohler et al 2013;Fernández et al 2013;Meng et al 2015) (Table 4).…”

Section: Application Of Dllme-hplc-pda Proceduressupporting

confidence: 85%

“…Although, there are few studies about the analysis of opium alkaloids and opioids drugs in clinical biological samples (Wey and Thorman 2001;Whittington and Kharasch 2003;Saraji et al 2011;Shamsipur and Fattahi 2011;Ranjbari et al 2012;Ahmadi-Jouibari et al 2013), but there are scant data about the analysis of opioids by DLLME-HPLC-PDA in postmortem urine samples. Therefore, in this study, we optimized a DLLME-HPLC-PAD for the extraction and determination of morphine, codeine, and methadone in postmortem urine samples.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of opioids in postmortem urine samples by dispersive liquid-liquid microextraction and high performance liquid chromatography with photo diode array detection

Alahyari

Setareh²,

Shekari³

et al. 2018

Egypt J Forensic Sci

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Background: Opioids abuse and related deaths are increasing in the world. Therefore, the design of new analytical methods for detection of opioids in biological samples is necessary for clinical and forensic settings. Methods: In this study, dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography with photo diode array detector (HPLC-PDA), as a new and sensitive method were examined for the extraction and determination of morphine, codeine and methadone in postmortem urine samples. Effective factors on DLLME were optimized. The extracts were analyzed by HPLC-PDA using a Eurospher® C 18 column (250 mm × 4.6 mm, particle size: 5 μm). Results: The volumes of chloroform as the extraction solvent and acetone as the dispersive solvent were selected 300 μl and 500 μl, respectively. The optimum pH 9.8 and extraction time was 0.5 min were selected. Under optimum condition, the enrichment factor and the recovery of morphine, codeine, and methadone spiked into postmortem urine samples were in the range of 175-215.8 and 87.5-107.9%, respectively. Calibration curves for each analyte are linear in the range of 0.5-100 μg ml

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Section: Application Of Dllme-hplc-pda Proceduressupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Analysis of opioids in postmortem urine samples by dispersive liquid-liquid microextraction and high performance liquid chromatography with photo diode array detection

Alahyari

Setareh²,

Shekari³

et al. 2018

Egypt J Forensic Sci

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“…Upon centrifugation of this solution, sedimented phase of chloroform was taken out, evaporated and reconstituted in methanol which was injected into HPLC for analysis. The method was rapid as it consumed less than five minutes for extraction of methadone [20]. Microextraction with packed sorbent was also reported for methadone, EDDP, buprenorphine (BUP) and norbuprenorphine (NBUP) in addition to other drugs of abuse, as described in ref [13].…”

Section: Analysis Of Opioidsmentioning

confidence: 99%

Microextraction Techniques for Forensic Drug Analysis in Saliva

Jain¹

2017

FRCIJ

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Forensic drug analysis involves trace level analysis of drugs of abuse and their metabolites in different biological matrices. Saliva has proven to be an alternative matrix over blood, plasma and urine, since collection of saliva is non-invasive, simple, and rapid. Additionally, concentration of drugs in saliva can be correlated to free drug concentration in plasma. Recently, great attention has been paid to develop microextraction techniques for analysis of drugs of abuse in saliva in order to improve quality and sensitivity of analysis. Microextraction techniques are rapid, eco-friendly, inexpensive, simple and offer high extraction efficiencies and enrichment factors. This article focuses on the reviews of procedures and applications of modern microextraction techniques for analysis of drugs of abuse in saliva samples.

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“…Overdose of methadone causes respiratory depression, hypertension, coma and some other symptoms. Various biological samples have been analyzed to monitor methadone levels, including serum (17)(18)(19)(20), plasma (21)(22)(23)(24)(25), whole blood (26), urine (17,18,21,23,24,(26)(27)(28)31), saliva (18,25), EB (14,30) and sweat (25) samples. Due to depressed levels of methadone in biological fluids and especially in EB due to the further dilution of evaporating water which is entrapped in the cooling system of the EB sample collection device, solid phase extraction (14,17,20,22,23,26,27,29,30), liquid phase extraction (25,31), or more sensitive detection like mass spectrometry (MS) method (14,21,22,28,30) has been applied for monitoring of methadone in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Analysis of Methadone in Exhaled Breath Condensate Using a Validated LC-UV Method

et al. 2015

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-Purpose. A combined microextraction and separation method is presented for the determination of methadone in exhaled breath condensate (EBC) which is a promising non-invasive biological component for monitoring drug concentrations. Methods. In this work, dispersive liquidliquid microextraction (DLLME) and ultrasonic liquid-liquid microextraction (ULLME) procedure coupled with a validated liquid chromatography method were used for analysis of methadone in EBC collected using an in-house cold trap setup. The method has been validated according to the FDA guidelines using EBC-spiked samples and tested on a number of EBC samples collected from patients. Results. The best DLLME conditions involved the use of a disperser solvent of methanol (1 mL), extraction solvent of chloroform (200 L), EBC sample pH of 10.0 and centrifugation at 6000 rpm for 5 minutes. The conditions for ULLME were 150 L of chloroform and the samples were sonicated for 4 minutes. The method was validated over the concentration range of 0.5-10 g/L -1 in EBC. Inter-and intra-day precision and accuracy were less than 5 % where the acceptable levels are less than 20%. Furthermore, the validated method was successfully applied for the determination of methadone in patients' EBC samples. Conclusions. The outcomes indicate that the developed LC-UV combined with DLLME and/or ULLME extraction methods can be employed for the extraction and separation of methadone in EBC samples.

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Preconcentration of trace amounts of methadone in human urine, plasma, saliva and sweat samples using dispersive liquid–liquid microextraction followed by high performance liquid chromatography

Cited by 67 publications

References 34 publications

Analysis of opioids in postmortem urine samples by dispersive liquid-liquid microextraction and high performance liquid chromatography with photo diode array detection

Analysis of opioids in postmortem urine samples by dispersive liquid-liquid microextraction and high performance liquid chromatography with photo diode array detection

Microextraction Techniques for Forensic Drug Analysis in Saliva

Extraction and Analysis of Methadone in Exhaled Breath Condensate Using a Validated LC-UV Method

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