Precolumn derivatization technique for high-performance liquid chromatographic determination of penicillins with fluorescence detection

On-line Vapor-Phase Generation Followed by Fourier-Transform Infrared Spectrometry for the Quantitative Analysis of Water-Soluble Penicillin G in Pharmaceutical Formulations

“…Samples were also analyzed by a reported HPLC method. 9 The results obtained by using a simple calibration line, as well as those found by the reference procedure agreed well (Table 4). These results demonstrate the validity of the proposed procedure for the determination of PENG in real samples.…”

“…9 Penicillin was derivatized by a two-step reaction to give a fluorescent derivative. The resulting mixture was injected directly onto a reversed-phase column and analyzed by HPLC.…”

“…Previous methods for measuring PENG in different real samples, such as pharmaceutical formulations, ovine milk, human plasma and wastewater, include methods based on spectrometry, [3][4][5] electrophoresis, 6,7 high-performance liquid chromatography (HPLC), 8,9 gas chromatography, 10 colorimetry, 11 and electrochemistry. 12 However, some of these methods are insensitive, tedious, time consuming or expensive.…”

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

“…The samples were deproteinized by ACN. For sample clean up, the supernatant was applied to a cation-exchange resin column (55 mm65.0 mm) packed with AG 50W-X8 (H + ) [41].…”

Fluorogenic and fluorescent labeling reagents with a benzofurazan skeleton

“…The suppression of the derivatization reaction by the metal ions could be avoided by the addition of ethylenediaminetetraacetic acid (EDTA) to the reaction medium (Miyano et al, 1985a). The derivatization using NBD-F as a pre-column fluorogenic reagent was applied to the determination of amino acids Imai et al, 1982;Imai, 1983, 1984;Kotaniguchi et al, 1987), amines, amino acids and imino acids , amines and proteins (Miyano et al, 1985b), hexosamines and hexosaminols (Imanari et al, 1985), neutral sugars as their glycamines (Shinomiya et al, 1987), aminophospholipids (Kaneko et al, 1987), Ssulfocysteine and S-sulfoglutathione (Togawa et al, 1988), phosphatidylethanolamine and its lysoform (Saris and Somerharju, 1989), amphetamine-related compounds (Hayakawa et al, 1989), selenium (Nakagawa et al, 1989), cyclamate (Hauck and Köbler, 1990), penicillins (Iwaki et al, 1990), ammonia (Kobayashi et al, 1992), peptides Arg-Gly-NH 2 and Arg-Pro-Lys-Pro (Yoshinaga et al, 1994), cholinergic channel activator A82695 (Hui and Marsh, 1995), dipeptides (Beijersten and Westerlund, 1995), hydrolyzed fumonisin B 1 (Scott and Lawrence, 1996), anatoxin-a (James and Sherlock, 1996;James et al, 1997), proteines (Wise et al, 1996), peptides (Kanazawa et al, 1997) (Jiménez and Mateo, 1997), anatoxin-a, homoanatoxin-a and their degradation products (James et al, 1998), methamphetamine and related compounds , imine-linked opines (Zhang et al, 1998), L-arginine (Prados et al, 1999), D-penicillamine (Al-Majed, 2000) and neurotoxin domic acid (James et al, 2000). Furthermore, after the derivatization with NBD-F, the enantioselective separation of amino acids were achieved on the chiral stationary phases ...…”