Precocious flowering and seeding behaviour in tissue-cultured bamboos

Nadgauda, R. S.; Parasharami, Varsha A.; Mascarenhas, A. F.

doi:10.1038/344335a0

Cited by 114 publications

(70 citation statements)

References 7 publications

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“…In many self-pollinating crops, seed setting followed by in vitro flowering has been previously reported (Asawaphan et al 2005;Bodhipadma and Leung 2003;Franklin et al 2000;Ignacimuthu et al 1997;Nadgauda et al 1990;Rao et al 2005;Saritha and Naidu 2007;Zhang 2007). Correspondingly, in the present study also mature lettuce seeds have developed in vitro ( Figure 1F).…”

Section: Transgenic Seed Setting In Vitrosupporting

confidence: 71%

“…Nevertheless, the type and concentration of PGRs critically affected the induction of in vitro flowering in many other plant species (Franklin et al 2000;Galoch et al 2002;Lin et al 2004;Taylor et al 2005;Vu et al 2006;Wang et al 2001). As the pattern of inflorescence development is differentially regulated by PGRs (Nadgauda et al 1990), which may result in the malformation of flower (Vu et al 2006), in vitro flowering in the absence of PGR is advantageous in breeding and to study the hormonal regulation of flowering and floral organ development.…”

Section: In Vitro Flowering Of Transgenic Shootsmentioning

confidence: 99%

“…Incidence of in vitro flowering has been reported in several plant species (Asawaphan et al 2005;Chang and Chang 2003;Franklin et al 2000;Galoch et al 2002;Hee et al 2007;Ignacimuthu et al 1997;Lin et al 2004;Ochatt and Sangwan 2008;Sivanesan and Jeong 2007;Vu et al 2006;Wang et al 2001). Commonly, flowering occurs more quickly under in vitro condition than the field condition in many species (Franklin et al 2000;Hee et al 2007;Ignacimuthu et al 1997;Nadgauda et al 1990;Ochatt and Sangwan 2008;Trinh et al 1987), which would be extremely useful for studies related to flowering of plants that have a long juvenile period under natural conditions (Hee et al 2007;Nadgauda et al 1990). In vitro flowering could also be used in breeding of plant species that show irregular pollen development or asynchronous flowering between and within varieties, which can greatly compromise the efficiency of breeding.…”

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confidence: 99%

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In vitro flowering and viable seed setting of transgenic lettuce cultures

Franklin

Oliveira

Dias

2011

Plant Biotechnology

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Production of transgenic lettuce seeds via in vitro flowering and fruit setting is reported here. Six days old cotyledons were co-cultivated with Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pCAMBIA2301 carrying the reporter gene a-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Transgenic calluses and shoot buds were induced on MS medium augmented with 0.1 mg l Ϫ1 benzyladenine (BA), 0.1 mg l Ϫ1 a-naphtaleneacetic acid (NAA), 100 mg l Ϫ1 kanamycin and 500 mg l Ϫ1 timentin (ticarcillin clavulanate). After transferring the cultures onto MS basal medium augmented or not with kanamycin, in vitro flower induction was observed within 90 days and eventually matured and produced seed pods. Pollen grains obtained from flowers induced on basal medium without kanamycin were heterogeneous containing both GUS positive and negative pollen grains. In the corresponding seed population, a Mendelian ratio (3 : 1) of gusA transgene segregation was observed. On the other hand, flowers that were induced on basal medium under kanamycin selection, all the pollen grains were GUS positives.

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Section: Transgenic Seed Setting In Vitrosupporting

confidence: 71%

Section: In Vitro Flowering Of Transgenic Shootsmentioning

confidence: 99%

mentioning

confidence: 99%

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In vitro flowering and viable seed setting of transgenic lettuce cultures

Franklin

Oliveira

Dias

2011

Plant Biotechnology

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“…The effect of coconut water on bamboo shoot multiplication has been reported by different authors. Nadgauda et al (1990) and Rajapakse, (1992) induced shoot proliferation in the medium supplemented with coconut water in seedling derived culture of B. bambos and D. brandisii and in D. giganteus respectively, for which in vitro flower were also induced. Saxena and Bhojwani, (1993) reported to use 10% coconut milk (CM) as additive for better shoot proliferation in D. giganteus.…”

Section: Resultsmentioning

confidence: 99%

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Raju

Roy

2017

Jahangirnagar Univ. J. Biol. Sci.

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Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog's (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.

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“…In vitro bamboo flowering was reported in Bambusa arundinacea, Dendrocalamus brandisii, and D. strictus as well as other species but in most of these reports the juvenile shoots that precociously flowered were derived from seedlings (Nadgauda et al, 1990;Ramanayake et al, 2001;Lin et al, 2003). However, we observed in vitro bamboo flowering that occurred in bamboo plants developed from bamboo tissue culture initiated in 1991 and thereafter subcultured continuously on culture medium.…”

Section: Discussionmentioning

confidence: 99%

Understanding bamboo flowering based on large-scale analysis of expressed sequence tags

Lin

Chow

Chen³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Unlike other plants, bamboo (Bambusoideae) flowering is an elusive physiological phenomena, because it is unpredictable, longperiodic, gregarious, and uncontrollable; also, bamboo plants usually die after flowering. The flowering mechanism in Arabidopsis thaliana, a eudicot model species, is well established, but it remains unknown in bamboo species. We found 4470 and 3878 expressed sequence tags in the flower bud and vegetative shoot cDNA libraries, respectively, of the bamboo species, Bambusa oldhamii. Different genes were found expressed in bamboo flower buds compared to vegetative shoots, based on the Munich Information Center for Protein Sequences functional categorization; flowering-related genes were also identified in this species. We also identified Arabidopsis flowering-specific homologs that are involved in its photoperiod in this bamboo species, along with autonomous, vernalization and gibberellin-dependent pathways, indicating that bamboos may have a similar mechanism to control floral transition. Some bamboo expressed sequence tags shared high similarity with those of rice, but others did not match any known sequences. Our data lead us to conclude that bamboo may have its own unique flowering genes. This information can help us understand bamboo flowering and provides useful experimental methods to study the mechanisms involved.

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Precocious flowering and seeding behaviour in tissue-cultured bamboos

Cited by 114 publications

References 7 publications

In vitro flowering and viable seed setting of transgenic lettuce cultures

In vitro flowering and viable seed setting of transgenic lettuce cultures

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Understanding bamboo flowering based on large-scale analysis of expressed sequence tags

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