Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Abstract

Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog's (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.

In vitro multiplication of woody bamboo in the Southwestern Amazon, Acre State, Brazil

“…Moreover, Raju and Roy (2016) studied Bambusa bambos and found high shoot induction in MS liquid medium supplemented with 2.0 mg L -1 BA and 1.0 mg L -1 TDZ, as well as a high average number of shoots (3.14) per explant, shoot multiplication rate and average shoot length using the same MS medium.…”

A Concise Review of Dendrocalamus asper and Related Bamboos: Germplasm Conservation, Propagation and Molecular Biology

“…According to reference [175], the juvenility of lateral shoots, the season of the cultures initiated and the position of axillary bud on the branch highly affect the bud break frequency in D. longispathus. Moreover, reference [213] reported that nodal segments from mature clumps of B. bambos with pre-existing axillary buds were primarily preferred as explants due to their sufficient availability all-yearround to initiate in vitro cultures, while reference [214] reported that explants from young lateral buds showed a bud break in B. tulda. Besides, explants from healthy mother stock were found to be good for the regeneration of new plants in D. hamiltonii [125], P. stocksii Munro [192], G. angustifolia and D. giganteus [119].…”

Regeneration Technique of Bamboo Species through Nodal Segments: A Review

“…Several researchers reported that the proliferation and shoot multiplication of the bamboo was successfully obtained under in vitro culture on semi-solid/solid MS media [34,35,36,39,[40][41][42][43][44][45][46][47][48]. Mostly, 0.8% agar was widely used as a gelling agent to solidified/semi-solidified the media which influenced the plant metabolism [32][33][34][35][36][37][38][39]76]. Some researchers also used phytagel (0.2%) or Gelrite (0.2-0.35%) to agar which influenced high bud breaking in B. wamin [72] and shoot proliferation in B. oldhamii [77].…”