Preclinical safety of topically administered nanostructured lipid carriers (NLC) for wound healing application: biodistribution and toxicity studies

Vairo, Claudia; Collantes, María; Quincoces, Gemma; Villullas, Silvia; Peñuelas, Iván; Pastor, Marta; Gil, Ana Gloria; Gaínza, Eusebio; Hernández, Rosa María; Igartua, Manoli; Gainza, Garazi

doi:10.1016/j.ijpharm.2019.118484

Cited by 30 publications

(17 citation statements)

References 19 publications

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“…The hydrophobic monolayer has an occlusive effect that prevents water evaporation, allowing a potentiation of local antibacterial activity while supporting the skin's moisture level. Both SLN and NLC also display good biocompatibility and tolerability and are currently widely used in skincare products [108][109][110].…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Ozonated Oils as Antimicrobial Systems in Topical Applications. Their Characterization, Current Applications, and Advances in Improved Delivery Techniques

Ugazio

Tullio²,

Binello

et al. 2020

Molecules

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The search for a wide spectrum of antimicrobial agents that can avoid resistance while maintaining reasonable side effects has led to ozonated oils experiencing an increase in scientific interest and clinical applications. The treatment of vegetable oils with ozone leads to the creation of a reservoir of ozone that slowly releases into the skin thanks to the fact that ozone can be held as ozonides of unsaturated fatty acids. Interest in the use of ozonated oils has meant that several ozonated-vegetable-oil-containing products have been commercialized as cosmetic and pharmaceutical agents, and in innovative textile products with antibacterial activity. New approaches to the delivery of ozonated oils have very recently appeared in an attempt to improve their characteristics and reduce drawbacks, such as an unpleasant odor, high viscosity and undesired effects on skin, including irritation and rashes. The present review focuses on the current status of delivery agents that use ozonated oils as antimicrobial agents in topical (dermal, skin, and soft tissues) treatments. Challenges and future opportunities for these delivery systems will also be discussed.

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Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Ozonated Oils as Antimicrobial Systems in Topical Applications. Their Characterization, Current Applications, and Advances in Improved Delivery Techniques

Ugazio

Tullio²,

Binello

et al. 2020

Molecules

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“…Among them, nanostructured lipid carriers (NLCs) have gained attention as promising second-generation of lipid-based delivery systems, allowing e cient drug delivery to target tissues. In particular, NLCs are capable of exerting a local effect when topically administered on a wound, as demonstrated by Vairo et al (23). Moreover, antimicrobial activity can be increased when anti-Pseudomonas antibiotics are loaded in these nanoparticles by enhancing the interaction between the bio lm-bearing colony and the antibiotics.…”

Section: Introductionmentioning

confidence: 93%

Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering

Chato‐Astrain

Chato-Astrain

Sánchez-Porras

et al. 2020

Preprint

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Background: Treatment of patients affected by severe burns is challenging, especially due to the high risk of Pseudomonas infection. In the present work, we have generated a novel model of bioartificial human dermis substitute by tissue engineering to treat infected wounds using fibrin-agarose biomaterials functionalized with nanostructured lipid carriers (NLCs) loaded with two anti-Pseudomonas antibiotics: sodium colistimethate (SCM) and amikacin (AMK). Results: Results show that the novel tissue-like substitutes have strong antibacterial effect on Pseudomonas cultures, directly proportional to the NLC concentration. Free DNA quantification, WST-1 and Caspase 7 immunohistochemical assays in the functionalized dermis substitute demonstrated that neither cell viability nor cell proliferation were affected by functionalization in most study groups. Furthermore, immunohistochemistry for PCNA and KI67 and histochemistry for collagen and proteoglycans revealed that cells proliferated and were metabolically active in the functionalized tissue with no differences with controls. When functionalized tissues were biomechanically characterized, we found that NLCs were able to improve some of the major biomechanical properties of these artificial tissues, although this strongly depended on the type and concentration of NLCs. Conclusions: These results suggest that functionalization of fibrin-agarose human dermal substitutes with antibiotic-loaded NLCs is able to improve the antibacterial and biomechanical properties of these substitutes with no detectable side effects. This opens the door to future clinical use of functionalized tissues.

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“…The topical applications of lipid-based nanoparticles have been explored in depth due to the occlusive nature of formulations. In vivo and ex vivo biodistribution studies of NLCs radiolabeled with 99 mTc were performed by Vairo et al Their developed NLCs were safe, remained on the wounds and were not absorbed for at least 24 h [ 141 ].…”

Section: Lipid-based Delivery Systems In Regenerative Medicinementioning

confidence: 99%

Lipid-Based Drug Delivery Systems in Regenerative Medicine

Filipczak

Yalamarty

et al. 2021

Materials

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The most important goal of regenerative medicine is to repair, restore, and regenerate tissues and organs that have been damaged as a result of an injury, congenital defect or disease, as well as reversing the aging process of the body by utilizing its natural healing potential. Regenerative medicine utilizes products of cell therapy, as well as biomedical or tissue engineering, and is a huge field for development. In regenerative medicine, stem cells and growth factor are mainly used; thus, innovative drug delivery technologies are being studied for improved delivery. Drug delivery systems offer the protection of therapeutic proteins and peptides against proteolytic degradation where controlled delivery is achievable. Similarly, the delivery systems in combination with stem cells offer improvement of cell survival, differentiation, and engraftment. The present review summarizes the significance of biomaterials in tissue engineering and the importance of colloidal drug delivery systems in providing cells with a local environment that enables them to proliferate and differentiate efficiently, resulting in successful tissue regeneration.

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Preclinical safety of topically administered nanostructured lipid carriers (NLC) for wound healing application: biodistribution and toxicity studies

Cited by 30 publications

References 19 publications

Ozonated Oils as Antimicrobial Systems in Topical Applications. Their Characterization, Current Applications, and Advances in Improved Delivery Techniques

Ozonated Oils as Antimicrobial Systems in Topical Applications. Their Characterization, Current Applications, and Advances in Improved Delivery Techniques

Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering

Lipid-Based Drug Delivery Systems in Regenerative Medicine

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