Preclinical evaluation of collagen type I scaffolds, including gelatin-collagen microparticles and loaded with a hydroglycolic Calendula officinalis extract in a lagomorph model of full-thickness skin wound

Abstract

Previously, we have developed collagen type I scaffolds including microparticles of gelatin-collagen type I (SGC) that are able to control the release of a hydroglycolic extract of the Calendula officinalis flower. The main goal of the present work was to carry out the preclinical evaluation of SGC alone or loaded with the C. officinalis extract (SGC-E) in a lagomorph model of full-thickness skin wound. A total of 39 rabbits were distributed in three groups, of 13 animals each. The first group was used to compare wound healing by secondary intention (control) with wound healing observed when wounds were grafted with SGC alone. Comparison of control wounds with wounds grafted with SGC-E was performed in the second group, and comparison of wounds grafted with SGC with wounds grafted with SGC-E was performed in the third group. Clinical follow-ups were carried in all animals after surgery, and histological and histomorphometric analyses were performed on tissues taken from the healed area and healthy surrounding tissue. Histological and histomorphometric results indicate that grafting of SGC alone favors wound healing and brings a better clinical outcome than grafting SGC-E. In vitro collagenase digestion data suggested that the association of the C. officinalis extract to SGC increased the SGC-E cross-linking, making it difficult to degrade and affecting its biocompatibility.

The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

“…CLO preserved collagen confirming others studies which already showed that CLO extract reduced collagen breakdown (Millán et al, 2016), and increased collagen concentration (Aro et al, 2015). In gingival fibroblasts, CLO inhibited matrix metalloproteinases (MMP)-2 (Saini et al, 2012).…”

Two‐dimensional and three‐dimensional models for studying atherosclerosis pathogenesis induced by periodontopathogenic microorganisms

“…Previous studies in our tissue engineering group have tested the autologous artificial connecting tissue model in tissue regeneration medicine in animal models to investigate the healing of wounds in oral mucosae . Likewise, collagen I scaffolds have been developed as release systems for calendula extracts for skin wound healing . Currently, we are developing non‐crosslinked collagen I scaffolds using freeze‐drying methods on transwell inserts by seeding HCAECs and culturing them for 7 days until confluence is reached.…”

Treatment with type-I collagen scaffolds in patients with venous ulcers. Case report

“…(11,12) Type-I collagen, the most abundant extracellular protein in the dermis, plays a key role in skin lesions as it guides cell migration, promotes the proliferation and differentiation of fibroblasts and keratinocytes, and sequesters and releases controlled growth factors that modulate wound closure and accelerate angiogenesis. (13) The Tissue Engineering Working Group of the Universidad Nacional de Colombia developed some type-I membranes or acellular collagen scaffolds that have helped close connective tissue wounds and have favored the regeneration of skin and oral mucosa wounds in preclinical models. (13)(14)(15)(16) To document their performance in cruciate areas, these membranes were used to treat two venous ulcers of different sizes in one patient.…”

“…(13) The Tissue Engineering Working Group of the Universidad Nacional de Colombia developed some type-I membranes or acellular collagen scaffolds that have helped close connective tissue wounds and have favored the regeneration of skin and oral mucosa wounds in preclinical models. (13)(14)(15)(16) To document their performance in cruciate areas, these membranes were used to treat two venous ulcers of different sizes in one patient. The closure time of the ulcers depended on the initial area of each lesion: the complete epithelization of the largest wound occurred at 16 weeks, while the smallest ulcer closed at 4 weeks.…”