Preclinical assessment of safety and efficacy of intravenous delivery of autologous adipose-derived regenerative cells (ADRCs) in the treatment of severe thermal burns using a porcine model

Foubert, Philippe; Liu, Mike; Anderson, Samantha F.; Rajoria, Rohit; Gutierrez, Damian; Zafra, Diana; Tenenhaus, Mayer; Fraser, John K.

doi:10.1016/j.burns.2018.05.006

Cited by 21 publications

(9 citation statements)

References 43 publications

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“…Both Zhang and Foubert [ 27 , 28 ] continuously reported changes in the white blood cell count of 27 animals at 0, 1, 2, 3, and 5 days post treatment (Fig. 5 ).…”

Section: Resultsmentioning

confidence: 86%

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Efficacy assessment of mesenchymal stem cell transplantation for burn wounds in animals: a systematic review

Wang

Yang

et al. 2020

Stem Cell Res Ther

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Background: Clinically, severe burns remain one of the most challenging issues, but an ideal treatment is yet absent. Our purpose is to compare the efficacy of stem cell therapy in a preclinical model of burn wound healing. Methods: Research reports on mesenchymal stem cells (MSCs) for burn wound healing were retrieved from 5 databases: PubMed, Embase, MEDLINE, Web of Science, and the Cochrane Library. The primary outcomes reported in this article include the un-healing rate of the wound area, the closure rate, and the wound area. Secondary outcomes included CD-31, vascular density, interleukin (IL)-10, thickness of eschar tissue, vascular endothelial growth factor (VEGF), and white blood cell count. Finally, a subgroup analysis was conducted to explore heterogeneity that potentially impacted the primary outcomes. A fixed-effects model with a 95% confidence interval (CI) was performed when no significant heterogeneity existed. Otherwise, a random-effects model was used. All data analysis was conducted by using Engauge Digitizer 10.8 and R software. Results: Twenty eligible articles were finally included in the analysis. Stem cell therapy greatly improved the closure rate (2.00, 95% CI 0.52 to 3.48, p = 0.008) and compromised the wound area (− 2.36; 95% CI − 4.90 to 0.18; p = 0.069) rather than the un-healing rate of the wound area (− 11.10, 95% CI − 32.97 to 10.78, p = 0.320). Though p was 0.069, there was a trend toward shrinkage of the burn wound area after stem cell therapy. Vascular density (4.69; 95% CI 0.06 to 9.31; p = 0.047) and thickness of eschar tissue (6.56, 95% CI 1.15 to 11.98, p = 0.017) were also discovered to be significantly improved in the burn site of stem cell-treated animals. Moreover, we observed that animals in the stem cell group had an increased white blood cell count (0.84, 95% CI 0.01 to 1.66, p = 0.047) 5 days post treatment. Other indicators, such as VEGF (p = 0.381), CD-31 (p = 0.335) and IL-10 (p = 0.567), were not significantly impacted. Conclusions: Despite limited data from preclinical trials, this meta-analysis suggests that stem cell therapy is curative in decreasing the burn wound area and provides some insights into future clinical studies of stem cell therapy for burns.

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“…Both Zhang and Foubert [ 27 , 28 ] continuously reported changes in the white blood cell count of 27 animals at 0, 1, 2, 3, and 5 days post treatment (Fig. 5 ).…”

Section: Resultsmentioning

confidence: 86%

“…Interestingly, we observed a negative result in CD-31 staining (10.02, 95% CI − 10.33 to 30.37, p = 0.335), which was contradictory to the pooled analysis of vascular density. Both Zhang and Foubert [27,28] continuously reported changes in the white blood cell count of 27 Fig. 2 Primary outcome of closure rate.…”

Section: Secondary Resultsmentioning

confidence: 99%

Efficacy assessment of mesenchymal stem cell transplantation for burn wounds in animals: a systematic review

Wang

Yang

et al. 2020

Stem Cell Res Ther

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“…[119][120][121] Notably, big variations exist in these studies, such as cell source, animal model, and cell delivery method. Interestingly, in most studies, the outcomes are quite similar: MSCs promoted not only the healing of acute wounds and thermal burns [101][102][103][104][105][106][107] but also the healing of chronic wounds (Fig. 1), typically with an accelerated wound closure and improved healing quality (Table 2).…”

Section: Animal Studiesmentioning

confidence: 94%

“…A large number of animal studies have been performed to determine the therapeutic potential of MSCs for different skin wounds, including acute skin wounds, [101][102][103][104] thermal burns, [105][106][107] diabetic wounds, 49,[108][109][110][111][112] radiation burns, [113][114][115][116][117][118] and pressure sores. [119][120][121] Notably, big variations exist in these studies, such as cell source, animal model, and cell delivery method.…”

Section: Animal Studiesmentioning

confidence: 99%

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Mesenchymal Stem Cells for Chronic Wound Healing: Current Status of Preclinical and Clinical Studies

Huang

Gou

et al. 2020

Tissue Engineering Part B: Reviews

124

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Healing skin wounds with anatomic and functional integrity, especially under chronic pathological conditions, remain an enormous challenge. Due to their outstanding regenerative potential, mesenchymal stem cells (MSCs) have been explored in many studies to determine the healing ability for difficult-to-treat diseases. In this article, we review current animal studies and clinical trials of MSC-based therapy for chronic wounds, and discuss major challenges that confront future clinical applications. We found that a wealth of animal studies have revealed the versatile roles and the benefits of MSCs for chronic wound healing. MSC treatment results in enhanced angiogenesis, facilitated reepithelialization, improved granulation, and accelerated wound closure. There are some evidences of the transdifferentiation of MSCs into skin cells. However, the healing effect of MSCs depends primarily on their paracrine actions, which alleviate the harsh microenvironment of chronic wounds and regulate local cellular responses. Consistent with the findings of preclinical studies, some clinical trials have shown improved wound healing after transplantation of MSCs in chronic wounds, mainly lower extremity ulcers, pressure sores, and radiation burns. However, there are some limitations in these clinical trials, especially a small number of patients and imperfect methodology. Therefore, to better define the safety and efficiency of MSC-based wound therapy, large-scale controlled multicenter trials are needed in the future. In addition, to build a robust pool of clinical evidence, standardized protocols, especially the cultivation and quality control of MSCs, are recommended. Altogether, based on current data, MSC-based therapy represents a promising treatment option for chronic wounds.

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Fat and Stromal Cells for Acute Burn Treatment

Brosset,

Alkhotani,

Boucher

et al. 2022

Plastic and Aesthetic Regenerative Surgery and Fat Grafting

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Preclinical assessment of safety and efficacy of intravenous delivery of autologous adipose-derived regenerative cells (ADRCs) in the treatment of severe thermal burns using a porcine model

Cited by 21 publications

References 43 publications

Efficacy assessment of mesenchymal stem cell transplantation for burn wounds in animals: a systematic review

Efficacy assessment of mesenchymal stem cell transplantation for burn wounds in animals: a systematic review

Mesenchymal Stem Cells for Chronic Wound Healing: Current Status of Preclinical and Clinical Studies

Fat and Stromal Cells for Acute Burn Treatment

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