Efficacy assessment of mesenchymal stem cell transplantation for burn wounds in animals: a systematic review

Abstract

Background: Clinically, severe burns remain one of the most challenging issues, but an ideal treatment is yet absent. Our purpose is to compare the efficacy of stem cell therapy in a preclinical model of burn wound healing. Methods: Research reports on mesenchymal stem cells (MSCs) for burn wound healing were retrieved from 5 databases: PubMed, Embase, MEDLINE, Web of Science, and the Cochrane Library. The primary outcomes reported in this article include the un-healing rate of the wound area, the closure rate, and the wound area. Secondary outcomes included CD-31, vascular density, interleukin (IL)-10, thickness of eschar tissue, vascular endothelial growth factor (VEGF), and white blood cell count. Finally, a subgroup analysis was conducted to explore heterogeneity that potentially impacted the primary outcomes. A fixed-effects model with a 95% confidence interval (CI) was performed when no significant heterogeneity existed. Otherwise, a random-effects model was used. All data analysis was conducted by using Engauge Digitizer 10.8 and R software. Results: Twenty eligible articles were finally included in the analysis. Stem cell therapy greatly improved the closure rate (2.00, 95% CI 0.52 to 3.48, p = 0.008) and compromised the wound area (− 2.36; 95% CI − 4.90 to 0.18; p = 0.069) rather than the un-healing rate of the wound area (− 11.10, 95% CI − 32.97 to 10.78, p = 0.320). Though p was 0.069, there was a trend toward shrinkage of the burn wound area after stem cell therapy. Vascular density (4.69; 95% CI 0.06 to 9.31; p = 0.047) and thickness of eschar tissue (6.56, 95% CI 1.15 to 11.98, p = 0.017) were also discovered to be significantly improved in the burn site of stem cell-treated animals. Moreover, we observed that animals in the stem cell group had an increased white blood cell count (0.84, 95% CI 0.01 to 1.66, p = 0.047) 5 days post treatment. Other indicators, such as VEGF (p = 0.381), CD-31 (p = 0.335) and IL-10 (p = 0.567), were not significantly impacted. Conclusions: Despite limited data from preclinical trials, this meta-analysis suggests that stem cell therapy is curative in decreasing the burn wound area and provides some insights into future clinical studies of stem cell therapy for burns.

Bone marrow mesenchymal stem cells enhance angiogenesis and promote fat retention in fat grafting via polarized macrophages

“…Stem cells act through multiple mechanisms and improve the efficacy of stem cell therapy [ 18 – 20 ]. In addition to the direct transformative and regenerative abilities of transplanted cells, the regulation of immune cells by stem cells plays a key role in tissue regeneration [ 21 ].…”

Peran terapi mesenchymal stem cell (MSC) dalam penatalaksanaan luka bakar: sebuah tinjauan sistematis

“…1,2 Berdasarkan data WHO, kasus mortalitas akibat luka bakar diperkirakan sekitar 265.000 jiwa setiap tahunnya. 2 Dari studi epidemiologi di Rumah Sakit Cipto Mangunkusumo (RSCM) tahun 2011-2012 data pasien yang dirawat selama periode 2 tahun adalah 303 pasien dengan sebagian besar pasien dengan luka bakar berat sebanyak 45,87%. Sedangkan data dari RSUP Sanglah Denpasar tahun 2012 dari total 154 pasien yang dirawat, sebanyak 13 orang meninggal (8,42%) akibat ledakan api dengan luka bakar luas dan dalam.…”

“…Selain itu timbulnya jaringan parut dan kontraktur juga akan mempengaruhi fungsi pasien seperti terbatasnya ruang gerak sendi. 2,4 Oleh karena itu dibutuhkan suatu inovasi dalam penatalaksanaan kasus luka bakar untuk menghasilkan waktu penyembuhan yang lebih cepat dan luaran klinis yang lebih memuaskan. Metode yang sedang diteliti saat ini adalah penggunaan sel punca atau stem cell.…”

Mesenchymal stem cells against intestinal ischemia–reperfusion injury: a systematic review and meta-analysis of preclinical studies

“…MSCs are pluripotent cells with extensive self-renewal potential and can differentiate into various non-hematopoietic cells (osteoblast, chondrocyte, myocyte, adipocyte, hepatocyte, fibroblasts, enterocyte, neurocyte, endothelium, tendon, and ligament) under certain conditions [5]. In recent years, a growing body of research has reported MSCs can contribute to healing of injured tissues and curing many diseases [6][7][8][9][10] by inhibition of apoptosis, inflammation, and fibrosis, promotion of angiogenesis, release of repair factors, and immunomodulation [11,12].…”