Precision medicine in the allergy clinic: the application of component resolved diagnosis

Panaitescu, Carmen; Haidar, Laura; Buzan, Maria Roxana; Grijincu, Manuela; Spanu, Daniela; Cojanu, Cătălina; Laculiceanu, Alexandru; Bumbăcea, Roxana Silvia; Agache, Ioana

doi:10.1080/1744666x.2022.2034501

Cited by 5 publications

(2 citation statements)

References 214 publications

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“…Such allergen molecules are important tools for improving ragweed allergy management, and in particular, for diagnosis. Furthermore, they can serve as benchmark molecules for the development of innovative molecular allergy vaccines [36][37][38]44]. Currently, 11 allergens have been described for ragweed pollen [16] and Amb a 1 is considered the major allergen, with more than 90% of ragweed allergic patients sensitized to it [2,17,45].…”

Section: Discussionmentioning

confidence: 99%

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Buzan,

Grijincu,

Zbîrcea

et al. 2024

IJMS

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Common ragweed pollen allergy has become a health burden worldwide. One of the major allergens in ragweed allergy is Amb a 1, which is responsible for over 90% of the IgE response in ragweed-allergic patients. The major allergen isoform Amb a 1.01 is the most allergenic isoform in ragweed pollen. So far, no recombinant Amb a 1.01 with similar allergenic properties to its natural counterpart (nAmb a 1.01) has been produced. Hence, this study aimed to produce a recombinant Amb a 1.01 with similar properties to the natural isoform for improved ragweed allergy management. Amb a 1.01 was expressed in insect cells using a codon-optimized DNA construct with a removable N-terminal His-Tag (rAmb a 1.01). The recombinant protein was purified by affinity chromatography and physicochemically characterized. The rAmb a 1.01 was compared to nAmb a 1.01 in terms of the IgE binding (enzyme-linked immunosorbent assay (ELISA), immunoblot) and allergenic activity (mediator release assay) in well-characterized ragweed-allergic patients. The rAmb a 1.01 exhibited similar IgE reactivity to nAmb a 1.01 in different IgE-binding assays (i.e., IgE immunoblot, ELISA, quantitative ImmunoCAP inhibition measurements). Furthermore, the rAmb a 1.01 showed comparable dose-dependent allergenic activity to nAmb a 1.01 regarding basophil activation. Overall, the results showed the successful expression of an rAmb a 1.01 with comparable characteristics to the corresponding natural isoform. Our findings provide the basis for an improvement in ragweed allergy research, diagnosis, and immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Buzan,

Grijincu,

Zbîrcea

et al. 2024

IJMS

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“…Sensitivity to these as well as to other molecular mite components makes it possible to perform the component-resolved molecular diagnostics of allergy [ 14 ]. This allows the clinician to understand the individual sensitization details of a particular patient and, thus, may lead to prescription of a better treatment regimen to patients with an HDM allergy [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular sensitization pattern to house dust mites is formed from the first years of life and includes group 1, 2, Der p 23, Der p 5, Der p 7 and Der p 21 allergens

et al. 2023

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Background As the process and nature of developing sensitivity to house dust mites (HDMs) remain not fully studied, our goal was to establish the pattern, nature and timeframe of house dust mite (HDM) sensitization development in patients in Ukraine as well as the period when treatment of such patients would be most effective. Methods The data of the multiplex allergy test Alex2 was collected from 20,033 patients. To determine age specifics of sensitization, descriptive statistics were used. Bayesian Network analysis was used to build probabilistic patterns of individual sensitization. Results Patients from Ukraine were most often sensitized to HDM allergens of group 1 (Der p 1, Der f 1) and group 2 (Der p 2, Der f 2) as well as to Der p 23 (55%). A considerable sensitivity to Der p 5, Der p 7 and Der p 21 allergens was also observed. The overall nature of sensitization to HDM allergens among the population of Ukraine is formed within the first year of life. By this time, there is a pronounced sensitization to HDM allergens of groups 1 and 2 as well as to Der p 23. Significance of sensitization to Der p 5, Der p 7 and Der p 21 allergens grows starting from the age of 3–6. Bayesian Network data analysis indicated the leading role of sensitization to Der p 1 and Der f 2. While developing the sensitivity to group 5 allergens, the leading role may belong to Der p 21 allergen. Conclusion The results obtained indicate the importance of determining the sensitization profile using the multi-component approach. A more detailed study of the optimal age for AIT prescription is required as the pattern of sensitization to HDMs is formed during the first year of life.

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Ockham’s Razor: The Application of Parsimonious Medicine in Allergy/Immunology

Warner

2024

The Journal of Allergy and Clinical Immunology: In Practice

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Precision medicine in the allergy clinic: the application of component resolved diagnosis

Cited by 5 publications

References 214 publications

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Molecular sensitization pattern to house dust mites is formed from the first years of life and includes group 1, 2, Der p 23, Der p 5, Der p 7 and Der p 21 allergens

Ockham’s Razor: The Application of Parsimonious Medicine in Allergy/Immunology

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