Precise method for identification and study of individual cells by light and scanning electron microscopy

Rickberg, A. B.; Katsen, A. D.; Chumak, S. M.

doi:10.1002/sca.4950060406

Cited by 6 publications

(2 citation statements)

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“…The encircling methods described can be improved when a smaller area is selected or even better when whole individual cells or pollen grains are marked (van Ewijk & Mulder, 1976;Rickberg et al, 1984;Peat, 1981).…”

Section: Whole Cells a N D Pollen Grainsmentioning

confidence: 99%

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Techniques for combining light microscopy and scanning electron microscopy: a survey of the literature

Wouters

1987

Journal of Microscopy

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SUMMARY A survey of methods combining light microscopy and scanning electron microscopy is presented. A simple correlation is made when two preparations from adjacent parts of one specimen are investigated in two different microscopes. A more sophisticated method is the consecutive investigation of one specimen with two microscopes. A major problem in this method is the relocation of the area of interest. Several authors have presented solutions for this problem. It is preferable when one preparation is investigated in only one instrument, combining the two microscopical (LM and SEM) techniques, thus making relocation redundant.

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Section: Whole Cells a N D Pollen Grainsmentioning

confidence: 99%

“…Another method (Rickberg et al, 1984) marks individual cells grown on glass. Here a diamond indentor, attached to a gauge of microhardness, is pressed down beside a chosen cell.…”

Section: Whole Cells a N D Pollen Grainsmentioning

confidence: 99%

Techniques for combining light microscopy and scanning electron microscopy: a survey of the literature

Wouters

1987

Journal of Microscopy

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Identification and study of the same cell in monolayer culture by different methods of light microscopy, scanning, and transmission electron microscopy. Application for cryodamaged cells examination

Katsen¹,

Tregubova²,

Andrushkevich³

et al. 1992

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Cells were cultivated on transparent conductive substrates. glass slides coated with indium oxide: individual cells were marked with a diamond indentor. Cell cultures were frozen ( -15"C), thawed, and then stained with fluorescent dyes to determine cell damage. The marked cells were examined by phase contrast. fluorescence, and Nomarski DIC microscopy. After aldehyde and osmium tetroxide fixation, the cell preparations were sequentially treated with tannic acid, uranyl acetate, and lead citrate. The same marked cell could be sequentially studied by light microscopy (LM; in water immersion conditions), scanning electron microscopy (SEM; after dehydration and critical point drying), and transmission electron microscopy (TEM: after embedding of cell samples in epoxy resin and laser marking of the cell previously marked with a diamond indentor). The method used ensures good preservation of cell morphology, cell surface relief, and intracellular structures. The treatment used renders the cells conductive and permitted SEM of uncoated culture cells on conductive substrates.

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Cytotoxicity of the Diethyldithiocarbamate Anion, of its S-Methylester and of Respective Platinum (II) Complexes

Carrara¹,

Cima²,

Zampiron³

et al. 1988

Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy

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Precise method for identification and study of individual cells by light and scanning electron microscopy

Cited by 6 publications

References 2 publications

Techniques for combining light microscopy and scanning electron microscopy: a survey of the literature

Techniques for combining light microscopy and scanning electron microscopy: a survey of the literature

Identification and study of the same cell in monolayer culture by different methods of light microscopy, scanning, and transmission electron microscopy. Application for cryodamaged cells examination

Cytotoxicity of the Diethyldithiocarbamate Anion, of its S-Methylester and of Respective Platinum (II) Complexes

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