Prebiotic potential of natural gums and starch for bifidobacteria of variable origins

Modrackova, Nikol; Makovska, Marie; Mekadim, Chahrazed; Vlková, Eva; Tejnecký, Václav; Bolechová, Petra; Bunešová, Věra

doi:10.1016/j.bcdf.2019.100199

“…hallii DSM 3553 and Anaerostipes caccae DSM 14662 (all from human adult faeces) were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Germany) or from the strain collection of the Department of Microbiology, Nutrition and Dietetics at the Czech University of Life Sciences (CZU, Czechia). Isolates CZU 1000, CZU 1006 and CZU 1007 were identified using MALDI‐TOF MS as described (Modrackova et al ., 2019). Bacteria were reactivated from a glycerol stock stored at −80°C and routinely cultivated in Hungate tubes using anaerobically prepared, modified yeast extract‐casitone‐fatty acid medium (mYCFA, Duncan et al ., 2002) containing 35 mM acetate and 40 mM glucose as carbon source, and CO 2 in the gas phase.…”

Section: Methodsmentioning

confidence: 99%

Initial butyrate producers during infant gut microbiota development are endospore formers

Appert

¹

,

Garcia

²

,

Frei

³

et al. 2020

Environmental Microbiology

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The acquisition of the infant gut microbiota is key to establishing a host-microbiota symbiosis. Microbially produced metabolites tightly interact with the immune system, and the fermentation-derived short-chain fatty acid butyrate is considered an important mediator linked to chronic diseases later in life. The intestinal butyrate-forming bacterial population is taxonomically and functionally diverse and includes endospore formers with high transmission potential. Succession, and contribution of butyrate-producing taxa during infant gut microbiota development have been little investigated. We determined the abundance of major butyrate-forming groups and fermentation metabolites in faeces, isolated, cultivated and characterized the heat-resistant cell population, which included endospores, and compared butyrate formation efficiency of representative taxa in batch cultures. The endospore community contributed about 0.001% to total cells, and was mainly composed of the pioneer butyrateproducing Clostridium sensu stricto. We observed an increase in abundance of Faecalibacterium prausnitzii, butyrate-producing Lachnospiraceae and faecal butyrate levels with age that is likely explained by higher butyrate production capacity of contributing taxa compared with Clostridium sensu stricto. Our data suggest that a successional arrangement and an overall increase in abundance of butyrate forming populations occur during the first year of life, which is associated with an increase of intestinal butyrate formation capacity.

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“…Bifidobacterial strains (n = 115) of the species B. adolescentis , B. animalis , B. bifidum , B. breve , B. dentium , B. longum , and B. catenutalum , and B. pseudocatenulatum were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Germany) or the strain collection of the Department of Microbiology, Nutrition, and Dietetics (CZU, Czechia) ( Table 1 ). Strain identity was confirmed with MALDI-TOF MS (Bruker Daltonik GmbH, Germany) according to Modrackova et al (2019) [ 23 ]. MALDI-TOF MS failed to distinguish B. catenulatum and B. pseudocatenulatum , and to identify subspecies of B. longum .…”

Section: Methodsmentioning

confidence: 99%

Bifidobacterium β-Glucosidase Activity and Fermentation of Dietary Plant Glucosides Is Species and Strain Specific

Modrackova

¹

,

Vlková

²

,

Tejnecký

³

et al. 2020

Self Cite

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Dietary plant glucosides are phytochemicals whose bioactivity and bioavailability can be modified by glucoside hydrolase activity of intestinal microbiota through the release of acylglycones. Bifidobacteria are gut commensals whose genomic potential indicates host-adaption as they possess a diverse set of glycosyl hydrolases giving access to a variety of dietary glycans. We hypothesized bifidobacteria with β-glucosidase activity could use plant glucosides as fermentation substrate and tested 115 strains assigned to eight different species and from different hosts for their potential to express β-glucosidases and ability to grow in the presence of esculin, amygdalin, and arbutin. Concurrently, the antibacterial activity of arbutin and its acylglycone hydroquinone was investigated. Beta-glucosidase activity of bifidobacteria was species specific and most prevalent in species occurring in human adults and animal hosts. Utilization and fermentation profiles of plant glucosides differed between strains and might provide a competitive benefit enabling the intestinal use of dietary plant glucosides as energy sources. Bifidobacterial β-glucosidase activity can increase the bioactivity of plant glucosides through the release of acylglycone.

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“…angulatum and B. pseudocatenulatum were isolated from faecal samples of patas and Diana monkeys [16].…”

Section: Full-textmentioning

confidence: 99%

“…In addition, B. pseudolongum has been described in the faeces of captive chimpanzees [14]. Moreover, B. angulatum and B. pseudocatenulatum were isolated from faecal samples of patas and Diana monkeys [16].…”

mentioning

confidence: 99%

“…Despite efforts to isolate novel species from Old World monkeys, B. moukalabense is the only such newly identified bacterial species, isolated from the faeces of wild western lowland gorilla [13]. Multi-host species described in chimpanzees, the closest relatives of humans, include B. angulatum, B. catenulatum, B. pseudocatenulatum and B. dentium, which are also found in human stools [14][15][16]. In addition, B. pseudolongum has been described in the faeces of captive chimpanzees [14].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Five novel bifidobacterial species isolated from faeces of primates in two Czech zoos: Bifidobacterium erythrocebi sp. nov., Bifidobacterium moraviense sp. nov., Bifidobacterium oedipodis sp. nov., Bifidobacterium olomucense sp. nov. and Bifidobacterium panos sp. nov.

Bunešová

¹

,

Lugli

²

,

Modrackova

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Five Bifidobacterium strains, VB23T, VB24T, VB25T, VB26T and VB31T, were isolated from chimpanzee (Pan troglodytes), cotton-top tamarin (Saguinus oedipus), Goeldi’s marmoset (Callimico goeldii), moustached tamarin (Saguinus mystax) and patas monkey (Erythrocebus patas), respectively, which were kept in two Czech zoos. These strains were isolated from faecal samples and were Gram-positive, non-motile, non-sporulating, anaerobic and fructose-6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA revealed close relatedness between VB23T and Bifidobacterium angulatum LMG 11039T (96.0 %), VB24T and Bifidobacterium pullorum subsp. pullorum DSM 20433T (96.1 %), VB25T and Bifidobacterium goeldii LMG 30939T (96.5 %), VB26T and Bifidobacterium imperatoris LMG 30297T (98.1 %), and VB31T and B . angulatum LMG 11039T (99.40 %). Internal transcribed spacer profiling revealed that VB23T, VB24T, VB25T, VB26T and VB31T had highest similarity to Bifidobacterium breve LMG 13208T (77.2 %), Bifidobacterium longum subsp. infantis ATCC 15697T (85.8 %), Bifidobacterium biavatii DSM 23969T (76.9 %), B. breve LMG 13208T (81.2 %) and B. angulatum LMG 11039T (88.2 %), respectively. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) analyses with their closest neighbours supported the independent phylogenetic positions of the strains with values between 86.3 and 94.3 % for ANI and 25.8 and 54.9 % for dDDH. These genomic and phylogenetic analyses suggested that the evaluated strains were novel Bifidobacterium species named Bifidobacterium erythrocebi sp. nov. (VB31T=DSM 109960T=CCUG 73843T), Bifidobacterium moraviense sp. nov. (VB25T=DSM 109958T=CCUG 73842T), Bifidobacterium oedipodis sp. nov. (VB24T=DSM 109957T=CCUG 73932T), Bifidobacterium olomucense sp. nov. (VB26T=DSM 109959T=CCUG 73845T) and Bifidobacterium panos sp. nov. (VB23T=DSM 109963T=CCUG 73840T).

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Prebiotic potential of natural gums and starch for bifidobacteria of variable origins

Cited by 16 publications

References 61 publications

Initial butyrate producers during infant gut microbiota development are endospore formers

Initial butyrate producers during infant gut microbiota development are endospore formers

Bifidobacterium β-Glucosidase Activity and Fermentation of Dietary Plant Glucosides Is Species and Strain Specific

Five novel bifidobacterial species isolated from faeces of primates in two Czech zoos: Bifidobacterium erythrocebi sp. nov., Bifidobacterium moraviense sp. nov., Bifidobacterium oedipodis sp. nov., Bifidobacterium olomucense sp. nov. and Bifidobacterium panos sp. nov.

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