Prebiotic effects of cassava bagasse in TNO's in vitro model of the colon in lean versus obese microbiota

Souza, Carlota Bussolo de; Roeselers, Guus; Troost, Freddy J.; Jonkers, Daisy; Koenen, M.; Venema, Koen

doi:10.1016/j.jff.2014.09.019

Cited by 36 publications

(18 citation statements)

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“…In this study, IN was found to increase the production of n -butyrate in the fermentations with the lean microbiota ( S2 Fig ) when compared to the obese. The butyrogenic effect of IN has been previously observed in vivo and in vitro [ 16 , 18 , 53 – 57 ]. n -Butyrate has been postulated as a molecule with health benefits for the human host since it has been found to be an important source of energy for colonocytes, with a potential protective role against colon cancer.…”

Section: Discussionmentioning

confidence: 99%

The Gut Microbiota from Lean and Obese Subjects Contribute Differently to the Fermentation of Arabinogalactan and Inulin

2016

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BackgroundAn aberrant metabolic activity or a compositional alteration of the gut microbiota has been proposed as a factor that makes us more prone to disease. Therefore, we explored the effect of two dietary fibers (arabinogalactan and inulin) on the microbiota from lean and obese subjects during 72 h in vitro fermentation experiments using the validated TNO dynamic in vitro model of the proximal colon: TIM-2. Metabolically, arabinogalactan fermentation showed a higher production of propionate when compared to n-butyrate in the obese microbiota fermentations. In general, lean microbiota produced more n-butyrate from the fermentation of both substrates when compared to the obese microbiota. Furthermore, the obese microbiota extracted more energy from the fermentation of both fibers.ResultsCompositionally, bacteria belonging to Gemmiger, Dorea, Roseburia, Alistipes, Lactobacillus and Bifidobacterium genera were found to be highly abundant or stimulated by the prebiotics in the lean microbiota suggesting a potential role in leanness. Furthermore, a significant correlation between known butyrogenic strains including B. adolescentis, an unclassified Bifidobacterium and F. prausnitzii with this metabolite in the fermentation of inulin in both microbiotas was found.ConclusionsAlthough supplementary in vivo studies are needed, the current study provides more evidence for the consumption of specific ingredients with the aim of modulating the gut microbiota in the context of obesity.

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Section: Discussionmentioning

confidence: 99%

The Gut Microbiota from Lean and Obese Subjects Contribute Differently to the Fermentation of Arabinogalactan and Inulin

2016

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“…Their results demonstrate a metabolic adaptation of the microbiota in response to the different nutrient loads together with a reorganization of the structure of the bacterial community. Moreover, our own recent studies add to knowledge by suggesting that not all substrates are fermented in an identical manner by the gut microbiota, as clearly shown by the different measurements of SCFA and branched-chain fatty acids (BCFA) observed in lean and obese microbiota pointing to the possible implications in energy extraction if similar effects happen in vivo [ 104 , 105 ]. On the other hand, Yang et al [ 106 ] observed non statistically significant differences in the microbiota activity from obese subjects when compared to lean after the in vitro fermentation with different dietary fibers, but small differences in propionate and butyrate production were found.…”

Section: Experimental Evidence On the Influence Of Gut Microbiota mentioning

confidence: 99%

“…Some other interesting studies such as the one from Bussolo de Souza et al [ 105 ] and Condezo-Hoyos et al [ 109 ] observed clear differences in lean and obese subjects at the compositional level when studying the effects of in vitro fermentation of fibers from cassava bagasse and apple cultivars, respectively. Cassava bagasse is a by-product from starch production and cassava flour.…”

Section: Experimental Evidence On the Influence Of Gut Microbiota mentioning

confidence: 99%

“…Cassava bagasse is a by-product from starch production and cassava flour. Bussolo de Souza et al [ 105 ] observed that cassava could modulate the microbiota composition from lean and obese individuals. The obese microbiota, in particular, became similar to the lean composition after the 72 h fermentation experiments which gave a nice indication of the improvement of the community’s health.…”

Section: Experimental Evidence On the Influence Of Gut Microbiota mentioning

confidence: 99%

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Does the Gut Microbiota Contribute to Obesity? Going beyond the Gut Feeling

Aguirre

Venema

2015

Microorganisms

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Increasing evidence suggests that gut microbiota is an environmental factor that plays a crucial role in obesity. However, the aetiology of obesity is rather complex and depends on different factors. Furthermore, there is a lack of consensus about the exact role that this microbial community plays in the host. The aim of this review is to present evidence about what has been characterized, compositionally and functionally, as obese gut microbiota. In addition, the different reasons explaining the so-far unclear role are discussed considering evidence from in vitro, animal and human studies.

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“…Various in vitro models of the gut microbiota have been used to examine the effects of prebiotics [9,10], probiotics [11], and dietary modulations [12] on the gut microbiota and its metabolites. In vitro models facilitate frequent sampling and increased reproducibility [13].…”

Section: Introductionmentioning

confidence: 99%

Selection of fast and slow growing bacteria from fecal microbiota using continuous culture with changing dilution rate

Adamberg

2018

Microbial Ecology in Health and Disease

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Background: Nutrient and energy metabolism in human colon depends on bacterial growth rate that is determined by the colonic transit rate.Objective: A novel approach, De-stat culture was used to distinguish the fast and slow growing sub-populations from fecal microbiota.Design: The enrichment and metabolism of bacteria from pooled fecal cultures of children was studied at dilution rates D = 0.2–0.0 1/h in mucin-supplemented media containing either arabinogalactan or apple pectin.Results: The study revealed clear differentiation of the fecal microbiota at higher (above 0.1 1/h) and lower (below 0.1 1/h) dilution rates, along with metabolic changes. Similarity of the fast and slow growing bacteria was observed in two different fecal pools and on both substrates, suggesting the dilution rate as the main triggering parameter for selection of bacteria. At high dilution rates, the species Collinsella aerofaciens, Dorea longicatena, Escherichia coli, Lachnoclostridium torques, and different Bacteroides (B. caccae, B. fragilis, B. ovatus, B. thetaiotaomicron, B. vulgatus) were dominant in both media variants. At low dilution rates, Akkermansia muciniphila, Eisenbergiella tayi, Negativicoccus succinivorans, and a group of Ruminococcaceae became dominant in both media and in both fecal pools. This change in bacterial population accompanied by the increased production of propionic and butyric acids as well as higher consumption of alanine and branched chain amino acids at low dilution rates.Conclusions: The study suggests that specific growth rate has important effect on the dynamics of colon microbiota. Manipulation of the proportions of fast and slow growing gut bacteria through modulation of the transit rate could be a target in human nutrition studies. The De-stat study would enable to predict changes in microbiota composition associated with the decrease or increase of the colonic transit rate.

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Prebiotic effects of cassava bagasse in TNO's in vitro model of the colon in lean versus obese microbiota

Cited by 36 publications

References 38 publications

The Gut Microbiota from Lean and Obese Subjects Contribute Differently to the Fermentation of Arabinogalactan and Inulin

The Gut Microbiota from Lean and Obese Subjects Contribute Differently to the Fermentation of Arabinogalactan and Inulin

Does the Gut Microbiota Contribute to Obesity? Going beyond the Gut Feeling

Selection of fast and slow growing bacteria from fecal microbiota using continuous culture with changing dilution rate

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