Prebiotic Effect of Lacto-N-biose I on Bifidobacterial Growth

Unique Sugar Metabolic Pathways of Bifidobacteria

“…22) Subsequently, Kiyohara et al demonstrated the prebiotic effect of LNB on many bifidobacteria, especially B. bifidum, B. breve, and B. longum. 36) On the other hand, LNB showed no prebiotic effect on other lactic acid and enteric bacteria (Bacteroides, Clostridium, Enterococcus, Eubacterium, Lactobacillus, Lactococcus, and Ruminococcus). Moreover, Xiao et al investigated the utilization of LNB by many strains of bifidobacteria (208 strains comprising 10 species and four subspecies).…”

“…106) D-Galactosyl-1,4-L-rhamnose was also produced using the corresponding phosphorylase. 107) Studies of the prebiotic effects of LNB on bifidobacteria in vitro 36,37) as well as its thermal decomposition mechanism 108) became possible only when the disaccharide became abundantly available due to this technology. LNB and GNB structures are found in biologically functional glycoconjugates, such as glycoproteins (O-glycans) and glycolipids (glycosphingolipids and blood type antigens).…”

Carbohydrate metabolism in Bifidobacteria

“…infantis, B. breve, and B. bifidum, strains, which are the predominant species in infant intestines [55,164]. Therefore, LNB is presumed to be a natural prebiotic for bifidobacteria [56]. The use of recombinant GLNBP from B. bifidum for large scale production of GNB, LNB and their derivatives, has recently been achieved [155,166] and it will be very interesting to determine their prebiotic potential.…”

Biological Analysis of the Microbial Metabolism of Hetero-Oligosaccharides in Application to Glycotechnology

“…Also, the remaining intestinal bacteria, Bacteroides, Clostridium, Enterococcus, Eubacterium, Propionibacterium, and Ruminococcus, were not significantly affected. 104) We have concluded that lacto-N-biose I is a highly specific natural prebiotic for bifidobacteria, which newborn infants receive for the first time from the colostrums of their mothers. We expect that lacto-Nbiose I supplementation will improve the quality of conventional synthetic milk.…”

“…We demonstrated the prebiotic effect of lacto-Nbiose I on bifidobacteria in vitro. 104) Pre-cultured cells were inoculated in GAM broth in the presence and the absence of 0.5% lacto-N-biose I. After 24 h of cultivation, cell growth was estimated.…”