Prebiotic activities of dextran from <i>Leuconostoc mesenteroides</i> SPCL742 analyzed in the aspect of the human gut microbial ecosystem

Kim, Geonhee; Bae, Jae Hoon; Cheon, Seongwon; Lee, Dong Hoon; Kim, Da Hye; Lee, Deukbuhm; Park, Sunghoon; Shim, S.B.; Seo, Jin‐Ho; Han, Nam Soo

doi:10.1039/d1fo03287a

Cited by 17 publications

(3 citation statements)

References 50 publications

(56 reference statements)

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“…provide a cross-feeding of dextran to probiotics, such as Bifidobacterium spp. [ 53 ]. CMPP might also regulate composition of gut microbiota and metabolic in this way.…”

Section: Resultsmentioning

confidence: 99%

Immunological Activity and Gut Microbiota Modulation of Pectin from Kiwano (Cucumis metuliferus) Peels

Zhu

Song

Martínez-Cuesta

et al. 2022

Foods

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For developing the recycling of fruit by-products from kiwano, a polysaccharide was extracted from kiwano (Cucumis metuliferus) peels, namely Cucumis metuliferus peels polysaccharide (CMPP), with the aim of investigating the potential beneficial effects. The composition of polysaccharides was analyzed by chemical methods. RAW264.7 macrophages cells and the microbiota dynamics simulator (BFBL gut model) were used for in vitro study. The result showed that CMPP mainly consists of glucuronic acid, arabinose, galactose and rhamnose. By intervening with RAW264.7 cells, CMPP promoted cell proliferation and showed immune-enhancing activity, which significantly (p < 0.05) induced the release of nitric oxide (NO), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) at a concentration of 50 μg/mL. In addition, CMPP had an impact on the composition of the gut bacteria, increasing the growth of Akkermansia, Bacteroides, Bifidobacterium, Feacalibacterium, and Roseburia. During the intake period, acetic, butyric and propionic acids were all increased, especially (p < 0.05) in the descending colon. Moreover, a decrease in ammonia concentration (10.17 ± 0.50 mM in the ascending colon, 13.21 ± 1.54 mM in the transverse colon and 13.62 ± 0.45 mM in the descending colon, respectively) was observed. In summary, CMPP can be considered as a pectin, showed immunological activity and function of gut microbiota modulation. This study could be the scientific basis of developing kiwano peels as beneficial to human health.

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“…provide a cross-feeding of dextran to probiotics, such as Bifidobacterium spp. [ 53 ]. CMPP might also regulate composition of gut microbiota and metabolic in this way.…”

Section: Resultsmentioning

confidence: 99%

Immunological Activity and Gut Microbiota Modulation of Pectin from Kiwano (Cucumis metuliferus) Peels

Zhu

Song

Martínez-Cuesta

et al. 2022

Foods

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“…Dextrans are potential new hydrocolloids with interesting rheological properties, improving the structure/texture of different foods (e.g., in the formulation of gluten-free bakery or low-fat dairy products; Lynch et al, 2018 ; Werning et al, 2022 ). In addition, the high molecular weight dextran produced by LAB strains could have also multiple beneficial properties for human health, since they can act as antiviral ( Nácher-Vázquez et al, 2015 ), antioxidant, hypocholesterolemic ( Nadzir et al, 2021 ) and prebiotic ( Kim et al, 2022 ) agents. Moreover, they have shown immunomodulatory ( Zarour et al, 2017 ), and anti-inflammatory ( Soeiro et al, 2016 ; Zhou et al, 2022 ) properties.…”

Section: Introductionmentioning

confidence: 99%

A methodology for the selection and characterization of riboflavin-overproducing Weissella cibaria strains after treatment with roseoflavin

et al. 2023

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Fermentative processes by lactic acid bacteria can produce metabolites of interest to the health and food industries. Two examples are the production of B-group vitamins, and of prebiotic and immunomodulatory dextran-type exopolysaccharides. In this study, three riboflavin- and dextran-producing Weissella cibaria strains (BAL3C-5, BAL3C-7 and BAL3C-22) were used to develop a new method for selection and isolation of spontaneous riboflavin-overproducing W. cibaria mutants. This method was based on the selection of strains resistant to roseoflavin. The DNA sequencing of the FMN riboswitch of bacterial cell populations treated with various roseoflavin concentrations, revealed the existence of at least 10 spontaneous and random point mutations at this location. Folding and analysis of the mutated FMN riboswitches with the RNA fold program predicted that these mutations could result in a deregulation of the rib operon expression. When the roseoflavin-treated cultures were plated on medium supporting dextran synthesis, the most promising mutants were identified by the yellow color of their mucous colonies, exhibiting a ropy phenotype. After their isolation and recovery in liquid medium, the evaluation of their riboflavin production revealed that the mutant strains synthesized a wide range of riboflavin levels (from 0.80 to 6.50 mg/L) above the wild-type level (0.15 mg/L). Thus, this was a reliable method to select spontaneous riboflavin-overproducing and dextran-producing strains of W. cibaria. This species has not yet been used as a starter or adjunct culture, but this study reinforces the potential that it has for the food and health industry for the production of functional foods or as a probiotic. Furthermore, analysis of the influence of FMN present in the growth medium, on rib mRNA and riboflavin levels, revealed which mutant strains produce riboflavin without flavin regulation. Moreover, the BAL3C-5 C120T mutant was identified as the highest riboflavin-overproducer. Determination of its chromosomal DNA sequence and that of BAL3C-5, revealed a total identity between the 2 strains except for the C120T mutation at the FMN riboswitch. To our knowledge, this work is the first demonstration that only a single alteration in the genome of a lactic acid bacteria is required for a riboflavin-overproducing phenotype.

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“…Recent research by Smith et al ( 2019 ) defined cross-feeding as ‘an interaction between bacterial strains in which molecules resulting from the metabolism of one strain are further metabolized by another strain’. Cross-feeding interactions between Bacteroides and other microorganisms have been well established (Luis et al 2018 , Murakami et al 2021 , Kim et al 2022 ). In the present study (Fig.…”

Section: Resultsmentioning

confidence: 99%

Comparative analysis of prebiotic effects of four oligosaccharides usingin vitrogut model: digestibility, microbiome, and metabolome changes

Cheon

Kim

Bae

et al. 2023

FEMS Microbiology Ecology

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Fructooligosaccharides (FOS), Ad-fructooligosaccharides (Ad-FOS), resistant maltodextrin (RMD), and maltooligosaccharides (MOS) are commercially available prebiotic oligosaccharides. In this study, the effects of prebiotics on the human gut microbial ecosystem were evaluated using an in vitro gut model. FOS and Ad-FOS showed tolerance to digestion, whereas RMD and MOS showed moderate digestion by digestive enzymes. In in vitro fecal fermentation, Bifidobacterium spp. increased in the following order: FOS, Ad-FOS, MOS, and RMD, whereas Bacteroides spp. increased in RMD medium. Bacteroides xylanisolvens exhibited cross-feeding by enabling the growth of other beneficial bacteria during co-culture in RMD medium. In metabolome analysis, total short-chain fatty acids (SCFAs) were highly produced in the following order: RMD, FOS, MOS, and Ad-FOS; acetate in the order of FOS, MOS/RMD, and Ad-FOS; butyrate in the order of RMD, MOS, FOS, and Ad-FOS; and propionate only in RMD. In addition, the conversion of betaine to trimethylamine was rarely affected in the following order: MOS, RMD, FOS, and Ad-FOS. Lastly, the four oligosaccharides inhibited the adhesion of pathogenic Escherichia coli to human epithelial cells to a similar extent. The comparative analysis results obtained in this study will provide comprehensive information of these substances to manufacturers and customers.

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Prebiotic activities of dextran from Leuconostoc mesenteroides SPCL742 analyzed in the aspect of the human gut microbial ecosystem

Abstract: Investigation of the prebiotic activities of LM742 dextran produced by Leuconostoc mesenteroides SPCL742 in the aspect of the human gut microbial ecosystem focusing on microbiome and metabolome changes in in vitro colonic fermentation.

Cited by 17 publications

References 50 publications

Immunological Activity and Gut Microbiota Modulation of Pectin from Kiwano (Cucumis metuliferus) Peels

Immunological Activity and Gut Microbiota Modulation of Pectin from Kiwano (Cucumis metuliferus) Peels

A methodology for the selection and characterization of riboflavin-overproducing Weissella cibaria strains after treatment with roseoflavin

Comparative analysis of prebiotic effects of four oligosaccharides usingin vitrogut model: digestibility, microbiome, and metabolome changes

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