Preadaptation of Protein Synthesis in Wheat Seedlings to High Temperature

Weidner, Manfred; Ziemens, Corinna

doi:10.1104/pp.56.5.590

Cited by 26 publications

(22 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Much more rapid are those adaptive responses to temperature shifts which are based upon conformational changes in preexisting enzymes (4,6,(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)14). Should conformational alterations of protein 3 C and 4 C structure due to a direct influence of AT on (incubation temperature).…”

Section: Resultsmentioning

confidence: 99%

“…The present paper characterizes further the phenomenon of phenotypical temperature adaptation of protein synthesis (16), mainly by establishing detailed time curves for readaptation. These kinetic data serve a dual purpose: first, they constitute indispensable information as such; and second, they are a means to gain certain insights about the involvement or noninvolvement of different biophysical and biochemical strategies in the adaptation process.…”

mentioning

confidence: 99%

“…Wheat seedlings were cultivated for 10 days at 20 C under continuous irradiation ( 150 ,IE. m-2 s-', Sylvania cool-white tubes) as described previously (16) and were then subjected to various 'This work was supported by Deutsche Forschungsgemeinschaft. temperature acclimation treatments.…”

mentioning

confidence: 99%

“…The incubation procedure, the separation of the total protein fraction, and the determination of the specific activity (pmol leucine/mg protein) were outlined in detail in an earlier paper (16). In case of P. typhoides, an additional trichloroacetic acid precipitation step was included.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Phenotypical Temperature Adaptation of Protein Synthesis in Wheat Seedlings

Weidner¹,

Combrink²

1979

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Optimum temperature and temperature coefficient of protein synthesis in young wheat plants exhibit phenotypical temperature adaptation. In plants grown for 2 days at either chilling (4 C), medium (20 C). or high (36 C) temperature P. typhoides (Rich.) seedlings (Babala grass) were soil grown at 25 C for 10 days under the same light conditions as the wheat and then exposed to 40 and 10 C, respectively, during a 2-day acclimation period. Thirty shoots were used for each in vivo protein synthesis experiment.L. minor was cultivated under sterile conditions in glass jars (15-cm diameter, 8-cm height) in 400 ml medium after Stewart (15). Cultures were diluted and transferred to a fresh medium as soon as the surface was completely covered with plants (7-10 days). Temperature and light conditions were identical with those for wheat. Logarithmically growing cultures were used for temperature adaptation experiments and exposed to 4 C (2 days ), 20 C (2 days), and 36 C (12 h Unless stated otherwise, uniformly labeled L-['4CJleucine was used, the 1 mm solution had a specific activity of either 0.33 ,uCi/ ,imol or 0.166 ,uCi/,umol. The incubation procedure, the separation of the total protein fraction, and the determination of the specific activity (pmol leucine/mg protein) were outlined in detail in an earlier paper (16). In case of P. typhoides, an additional trichloroacetic acid precipitation step was included. The incubation solutions were used repeatedly after sterile filtration (Sartorius membrane filter, type SM 11407, 0.2,um) until they turned brownish.All experiments were run at least in triplicate. Regression statistics were computed, using the facilities of the Rechenzentrum of University. RESULTS AND DISCUSSIONThe validity of the data from incorporation rates of externally supplied, radioactively labeled amino acids is above all dependent upon the following conditions. First, the rate of amino acid uptake into the metabolic pool(s) is not itself the rate-limiting step for the incorporation of label into protein. Second, the swamping of the metabolic pool(s) by application of rather high exogenous concentrations-which are necessary to bring the precursor pool within the shortest possible time into equilibrium with the added labeldoes not per se affect the in vivo rate of protein synthesis. By the following double-labeling experiment, it could be confirmed that the temperature dependence of ['4C]leucine incorporation into the total protein fraction represents the ,uS of in vivo protein synthesis.['4C]NaHCO3 (5 mCi/mmol) and [4,5-3H]

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Phenotypical Temperature Adaptation of Protein Synthesis in Wheat Seedlings

Weidner¹,

Combrink²

1979

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In an earlier study of temperature responses of protein synthesis in wheat seedlings, Weidner and Zeimans (22) found that the experimental temperatures evoking peak rates of protein synthesis increased concurrently with increasing temperature pretreatments. In addition, plants which had been exposed to higher temperatures demonstrated higher activation energies for protein synthesis than did plants grown at low temperature.…”

mentioning

confidence: 99%

Phenotypical Temperature Adaptation of Protein Turnover in Desert Annuals

Smrcka¹,

Szarek²

1986

Plant Physiol.

View full text Add to dashboard Cite

Protein synthesis and protein degradation rates were measured in three desert annual species at four different experimental temperatures. The taxa chosen for this study were Desert annual plants germinate and grow during different temperature regimes (20) and, therefore, provide an ideal system for investigating the mechanistic basis for such phenotypic variability. Gas exchange studies have shown that annual species adapted for growth during the hotter parts of the year demonstrate peak photosynthetic capacities at much higher temperatures than the winter annuals (7,19). Biophysical and biochemical differences also exist; e.g. chloroplastic membranes show a seasonal acclimation (7), and the relative thermostability of a key metabolic enzyme generally parallels increasing plant thermotolerance (14). These are consistent with other studies (1, 21) which demonstrate that, in general, plants adapted to lower temperature regimes have lower biochemical activity at high temperature than plants adapted to growth at higher temperatures.Protein turnover, defined as the rate of flux of amino acids through protein (9)

show abstract