Pre-column derivatization combined with UHPLC–MS/MS for rapid and sensitive quantification of bakuchiol in rat plasma

Zhuang, Xiaomei; Zhong, Yu-Huan; Yuan, Mei; Li, Hua

doi:10.1016/j.jpba.2012.11.001

Cited by 24 publications

(10 citation statements)

References 18 publications

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“…The matrix effect results from coelution of a target analyte with some components present in biological samples, which may or may not give a signal in SRM of the analyte but can affect its response dramatically . The matrix effects for l ‐THP and metabolites were assessed at low, medium, and high concentration levels.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous quantification of l‐tetrahydropalmatine and its urine metabolites by ultra high performance liquid chromatography with tandem mass spectrometry

Xiao¹,

Zhuang²,

Shen³

et al. 2014

J of Separation Science

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l-tetrahydropalmatine (l-THP) is a tetrahydroprotoberberine isoquinoline alkaloid that has been used as an analgesic agent in China for more than 40 years. Recent studies indicated its potential application in the treatment of drug addiction. In this study, a sensitive and rapid method using ultra high performance liquid chromatography with MS/MS was developed and validated for simultaneous quantitation of l-THP and its desmethyl metabolites. Enzymatic hydrolysis was integrated into sample preparation to enable the quantitative determination of both free and conjugated metabolites. Chromatographic separation was achieved on an Agilent Poroshell 120 EC-C18 column. Detection was performed by MS in the positive ion ESI mode. The calibration curves of the analytes were linear (r(2) > 0.9936) over the concentration range of 1-1000 ng/mL with the lower limit of quantification at 1 ng/mL. The precision for both intra- and interday determinations was <8.97%, and the accuracy ranged from -8.74 to 8.65%. The recovery for all the analytes was >70% without significant matrix effect. The method has been successfully applied to the urinary excretion study of l-THP in rats. The conjugates were found to be the major urine metabolites of the drug.

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Section: Resultsmentioning

confidence: 99%

Simultaneous quantification of l‐tetrahydropalmatine and its urine metabolites by ultra high performance liquid chromatography with tandem mass spectrometry

Xiao¹,

Zhuang²,

Shen³

et al. 2014

J of Separation Science

Self Cite

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“…1). LC-ESI-MS/MS analysis based on this product ion is highly selective and sensitive and is commonly used for MS/MS detection of dansylderivatives of phenolic compounds [28,29]. The efficiency of electrospray ionization (ESI-MS/MS) of APs without pre-column derivatization is much lower than those of derivatized APs.…”

Section: Introductionmentioning

confidence: 99%

Determination of alkylphenols in water samples using liquid chromatography–tandem mass spectrometry after pre-column derivatization with dansyl chloride

Pernica

Poloucká

Seifertová

et al. 2015

Journal of Chromatography A

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“…A variety of analytical methods have been developed to determine the bioactive components of Fructus Psoraleae including uorometric methods, 13 high-performance liquid chromatography with ultraviolet detection (HPLC-UV), [14][15][16] mass spectrometry (HPLC-MS) 17,18 or electrochemical detection (HPLC-ECD), 19 (MEKC), 20 and capillary electrophoresis coupled with mass spectrometry (CE-MS). 21 Most of the current methods mainly focused on the determination of one or two bioactive components in plants or crude formulations, and the pharmacokinetics studies of PR and IPR by HPLC-MS/MS 22,23 and SPE-HPLC-UV, 24 BCI by SPE-HPLC-UV 25 and BKC by UHPLC-MS/ MS. 26 These proposed methods have their own advantages and provide extensive choices for analysts. However, in general, the methods mentioned above suffer from the limited number of analytes, insufficient detection limits for biological samples, or complex pretreatments (SPE or pre-column derivatization).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and highly sensitive quantification of five bioactive components in Fructus Psoraleae and in rat plasma by HPLC with fluorescence detection

Chen

2014

Anal. Methods

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Psoralea corylifolia L. is an important medicinal herb that produces biologically active components. A simple and sensitive high performance liquid chromatography with fluorescence (HPLC-FL) method has been developed for the simultaneous quantification of five bioactive compounds including psoralen, isopsoralen, bavachin, bavachinin and bakuchiol in Fructus Psoraleae. The chromatographic separation was carried out on a C 18 column with gradient elution. The fluorescence properties of these five compounds were investigated. At the optimum detection wavelength (l ex /l em ¼ 280/405 nm), the analytes provided good fluorescence signals, and simultaneous and highly sensitive determination of these five components was achieved. The limits of detection (signal to noise ratio at 3) of five analytes were 0.8 ng mL À1 for psoralen, 2.5 ng mL À1 for isopsoralen, 1.2 ng mL À1 for bavachin, 2.1 ng mL À1 for bavachinin and 2.4 ng mL À1 for bakuchiol, which were lower than those for most of the current methods. Recoveries for all studied compounds in Fructus Psoraleae were between 93.7% and 108.2%.The proposed method was further applied to the analysis of rat plasma samples after oral administration of Fructus Psoraleae extraction. The results show that the developed HPLC-FL method is a very useful tool for the quality control of herbal medicine Psoralea corylifolia L., and for pharmacokinetics studies.

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Pre-column derivatization combined with UHPLC–MS/MS for rapid and sensitive quantification of bakuchiol in rat plasma

Cited by 24 publications

References 18 publications

Simultaneous quantification of l‐tetrahydropalmatine and its urine metabolites by ultra high performance liquid chromatography with tandem mass spectrometry

Simultaneous quantification of l‐tetrahydropalmatine and its urine metabolites by ultra high performance liquid chromatography with tandem mass spectrometry

Determination of alkylphenols in water samples using liquid chromatography–tandem mass spectrometry after pre-column derivatization with dansyl chloride

Simultaneous and highly sensitive quantification of five bioactive components in Fructus Psoraleae and in rat plasma by HPLC with fluorescence detection

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