Determination of alkylphenols in water samples using liquid chromatography–tandem mass spectrometry after pre-column derivatization with dansyl chloride

A method for the identification and quantification of bisphenol A and 12 bisphenol analogues in river water and sediment samples combining liquid-liquid extraction, precolumn derivatization, and ultra high-performance liquid chromatography coupled with tandem mass spectrometry was developed and validated. Analytes were extracted from the river water sample using a liquid-liquid extraction method. Dansyl chloride was selected as a derivatization reagent. Derivatization reaction conditions affecting production of the dansyl derivatives were tested and optimized. All the derivatized target compounds were well separated and eluted in 10 min. Dansyl chloride labeled compounds were analyzed using a high-resolution mass spectrometer with electrospray ionization in the positive mode, and the results were confirmed and quantified in the parallel reaction monitoring mode. The method validation results showed a satisfactory level of sensitivity. Linearity was assessed using matrix-matched standard calibration, and good correlation coefficients were obtained. The limits of quantification for the analytes ranged from 0.005 to 0.02 ng/mL in river water and from 0.15 to 0.80 ng/g in sediment. Good reproducibility of the method in terms of intra- and interday precision was achieved, yielding relative standard deviations of less than 10.1 and 11.6%, respectively. Finally, this method was successfully applied to the analysis of real samples.

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“…This finding is consistent with the derivatization temperatures reported by Pernica et al. and Vitku et al. .…”

Section: Resultssupporting

confidence: 93%

Simultaneous identification and quantification of bisphenol A and 12 bisphenol analogues in environmental samples using precolumn derivatization and ultra high performance liquid chromatography with tandem mass spectrometry

Wang

Yao

et al. 2018

J of Separation Science

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“…In the present work, in situ derivatization integrated with UADLLME in one step has been proposed to simplify the procedure and decrease the time of pretreatment. Compared with conventional SPE, LLE or centrifugation combined with precolumn derivatization methods, the derivatization time of this work was shortened by 2–60 times. The mean matrix effects were in the range 85.8–114.6% (Table ) and the recoveries were between 83.0% and 116.0% (see Supplementary Table S2, Supporting Information) in all cases, which indicated that the proposed method was accurate enough for the determination of EDCs in real samples.…”

Section: Resultsmentioning

confidence: 99%

“…The specific product ion of CCR had a larger value and hence better effect of matrix interference resistance than DNS‐Cl and benzoyl chloride. The derivatization reaction was performed at a temperature of 25°C within 1 min, while other derivatization agents commonly required 3–60 min, and the derivatization temperature had biological compatibility. It was proved that CCR was a very favorable derivatization reagent for researchers engaged in biological sample studies.…”

Section: Resultsmentioning

confidence: 99%

“…The most widely used derivatization reagents in GC/MS analysis of EDCs are N,O ‐bis(trimethylsilyl)trifluoroacetamide (BSTFA), BSTFA with trimethylchlorosilane (TMCS), N ‐methyl‐ N ‐(trimethylsilyl)trifluoroacetamide (MSTFA), pentafluorobenzyl bromide (PFBBr), acetic anhydride (AA), and MSTFA/ethanethiol/NH 4 I . Besides, several derivatization reagents have also been applied in the LC/MS/MS analysis of EDCs, such as dansyl chloride (DNS‐Cl), pyridine‐3‐sulfonyl chloride, and 10‐methylacridone‐2‐sulfonyl chloride (MASC) . However, these derivatization reagents have more or less limitations in their applications, such as poor stability, low detection sensitivity, operational inconvenience, difficult synthesis, or serious interferences in chromatograms.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Rapid and sensitive determination of multiple endocrine‐disrupting chemicals by ultrasound‐assisted in situ derivatization dispersive liquid–liquid microextraction coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Wei

Zheng

Wang

et al. 2017

Rapid Comm Mass Spectrometry

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“…Furthermore, the conversion of surfactants into volatile derivatives can be the source of errors due to the different kinetic properties of the oligomers and formation of by-products. To avoid the disadvantages, the high-performance liquid chromatography (HPLC) method has been employed with different detection systems [18]. Morales et al [19] reviewed the extraction, clean-up, separation and quantification systems for APEOs studying the LC with fluorescence detection (LC-FD), mass spectrometry (LC-MS) and especially LC with tandem MS (LC-MS2).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Non-Ionic Surfactant Triton X-100 Using Hydrophilic Interaction Liquid Chromatography and Mass Spectrometry

Farsang

Gaál²,

Horváth

et al. 2019

Molecules

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It is well known that surfactants increase the solubility of hydrophobic organic compounds and cause adverse environmental effects. The removal of these compounds from the contaminated soil or ground-water is particularly difficult due to their water soluble feature. In this work, an ultra-high performance hydrophilic interaction liquid chromatographic method was developed for the separation of oligomers of Triton X-100 octylphenol-polyethoxylate non-ionic surfactant. Liquid chromatography-mass spectrometry (LC-MS) was used to identify the Triton X-100 compounds. There was a 44 mass unit difference between two adjacent peaks that is the molar mass of one ethylene oxide group (–CH 2 CH 2 O–). A quadratic retention model was applied for the estimation of retention of the examined non-ionic surfactant and the optimization of gradient elution conditions. The optimized method was suitable for the baseline separation of 28 Triton X-100 oligomers in five minutes.

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Determination of alkylphenols in water samples using liquid chromatography–tandem mass spectrometry after pre-column derivatization with dansyl chloride

Cited by 39 publications

References 25 publications

Simultaneous identification and quantification of bisphenol A and 12 bisphenol analogues in environmental samples using precolumn derivatization and ultra high performance liquid chromatography with tandem mass spectrometry

Simultaneous identification and quantification of bisphenol A and 12 bisphenol analogues in environmental samples using precolumn derivatization and ultra high performance liquid chromatography with tandem mass spectrometry

Rapid and sensitive determination of multiple endocrine‐disrupting chemicals by ultrasound‐assisted in situ derivatization dispersive liquid–liquid microextraction coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Analysis of Non-Ionic Surfactant Triton X-100 Using Hydrophilic Interaction Liquid Chromatography and Mass Spectrometry

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